A5013559817- Microbial infections and disease research
- Salmonella and Campylobacter epidemiology
- Cancer Immunotherapy and Biomarkers
- Aquaculture disease management and microbiota
- Escherichia coli research studies
- Gut microbiota and health
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Identification and Quantification in Food
- Listeria monocytogenes in Food Safety
- Oral health in cancer treatment
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Yersinia bacterium, plague, ectoparasites research
- Colorectal and Anal Carcinomas
- Neuroendocrine Tumor Research Advances
- Gastrointestinal Tumor Research and Treatment
- Bacterial Identification and Susceptibility Testing
- Misinformation and Its Impacts
- Vector-Borne Animal Diseases
- Mathematical and Theoretical Epidemiology and Ecology Models
- Rabies epidemiology and control
- T-cell and Retrovirus Studies
National Veterinary Research Institute
2020-2025
In late 2022 and early 2023, SARS-CoV-2 infections were detected on three mink farms in Poland situated within a few km from each other. Whole-genome sequencing of the viruses two showed that they related to virus identified humans same region 2 years before (B.1.1.307 lineage). Many mutations found, including S protein typical adaptations host. The origin remains be determined.
Antimicrobial resistance (AMR) is one of the most important global health concerns; therefore, identification AMR reservoirs and vectors essential. Attention should be paid to recognition potential hazards associated with wildlife as this field still seems incompletely explored. In context, role free-living birds carriers noteworthy. Therefore, we applied methods used in monitoring, supplemented by colistin screening, investigate status Escherichia coli from coming natural habitats rescue...
The significance of Akkermansia bacteria presence in gut micobiome, mainly mucinifila, is currently being investigated the context supporting therapy and marker for response to immunotherapy cancer patients. It indicated that patients with non-small cell lung (NSCLC) treated immune checkpoint inhibitors (ICIs) respond better treatment if this bacterium present intestine.We performed next-generation sequencing microbiome from first or second line anti-PD-1 (anti-programmed death 1) anti-PD-L1...
Since April 2020, when the first SARS-CoV-2 infection was reported in mink and subsequently farm workers Netherlands, it has been confirmed that human-to-mink mink-to-human transmission can occur. Later, infections were many European North American countries.Samples from 590 a total of 28 farms tested by real-time RT-PCR. Whole genome sequences one positive generated genetic relatedness established.SARS-CoV-2 RNA detected on breeder with stock 5,850 mink. Active viraemia individually samples...
Enterococci as opportunistic bacteria are important for human health. Due to the prevalence and ease of acquisition transfer their genes, they an excellent indicator environmental contamination spread antimicrobial resistance. The aim study was assess Enterococcus spp. in wild birds Poland, determination susceptibility WGS analysis (E.) faecium E. faecalis. For this purpose, 138 samples from various species free-living were tested, with 66.7% positive results. Fourteen detected, faecalis...
The composition of the gut microbiome patients with advanced non-small cell lung cancer is currently considered a factor influencing effectiveness treatment immune checkpoint inhibitors. We aimed to evaluate baseline in before receiving first-line immunotherapy alone or combined chemoimmunotherapy. performed 16S rRNA sequencing based on hypervariable regions. Stool samples were collected from 52 NSCLC treated chemoimmunotherapy treatment. found that Ruminococcaceae family, species Alistipes...
Introduction: Factors other than PD-L1 (Programmed Death Ligand 1) are being sought as predictors for cancer immuno- or chemoimmunotherapy in ongoing studies and long-term observations. Despite high expression on tumor cells, some patients do not benefit from immunotherapy, while others, without the of this molecule, respond to immunotherapy. Attention has been paid composition gut microbiome a potential predictive factor immunotherapy effectiveness. Materials Methods: Our study enrolled 47...
Listeria monocytogenes, an important foodborne pathogen, may be present in different kinds of food and processing environments where it can persist for a long time. In this study, 28 L. monocytogenes isolates from fish manufactures were characterized by whole genome sequencing (WGS). Core multilocus sequence typing (cgMLST) analysis was applied to compare the with publicly available genomes strains recovered worldwide humans listeriosis. All but one (96.4%) examined belonged molecular...
Different species of Shewanella spp. widely inhabit freshwater and marine environments. Some them are opportunistic fish pathogens. The application high-throughput sequencing enabled the characterization taxonomic reclassification many species. Still, some strains collected from need to be better recognized. aim present study was classify determine phylogenetic relationships fish. complete genomes 94 different were sequenced using Illumina platform (MiSeq). 16S rRNA gene, genomic features...
Transmission of pathogenic and resistant bacteria from wildlife to the bacterial gene pool in nature affects ecosystem. Hence, we studied intestine content five wild rodent species: yellow-necked wood mouse (Apodemus flavicollis, n = 121), striped field agrarius, 75), common vole (Microtus arvalis, 37), bank (Myodes glareolus, 3), house (Mus musculus, 1) assess their potential role as an antimicrobial resistance (AMR) Salmonella vector. The methods adopted official AMR monitoring slaughtered...
The newest method of treatment for patients with NSCLC (non-small cell lung cancer) is immunotherapy directed at the immune checkpoints PD-1 (Programmed Cell Death 1) and PD-L1 Ligand 1). only validated predictor factor efficacy, but it imperfect. Some do not benefit from may develop primary or secondary resistance. This study aimed to assess intestinal resistome composition non-small cancer (NSCLC) treated checkpoint inhibitors in context clinical features potentially new prediction factors...
Characterization of the global genetic diversity bovine leukemia virus (BLV) is an ongoing international research effort. Up to now BLV sequences have been classified into eleven distinct genotypes. Although genotyping and molecular analysis field isolates were reported in many countries, there no report describing genotypes present cattle from Pakistan. In this study we examined 27 env gene BLV-infected coming four farms located Khyber Pakhtunkwa, Gilgit Baltisan Punjab provinces....
Many countries have reported severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) infections in mink, and transmission back to humans has raised the concern of novel variants emerging these animals. The monitoring system on Polish mink farms detected SARS-CoV-2 infection first January 2021 been kept place since then.
Background: Astroviruses (AstVs) are common pathogens of a wide range animal hosts, including mammals and avians, causing gastrointestinal diseases, mainly gastroenteritis diarrhea. They prompt significant health problem in newborns young children economic losses the poultry sector mink farms. Recent studies revealed growing number bat species carrying astroviruses with noticeable prevalence diversity. Here, we demonstrate first detection (BtAstVs) circulating population insectivorous bats...
Aeromonas sobria strain K928 was isolated from a common carp during Motile Infection/Motile Septicaemia disease outbreak on Polish fish farm and classified into the new provisional PGO1 serogroup. The lipopolysaccharide of A. subjected to mild acid hydrolysis, O-specific polysaccharide, which by gel-permeation chromatography, studied using sugar methylation analyses 1H 13C NMR spectroscopy. following structure branched polysaccharide repeating unit established....
Chlamydia gallinacea is an intracellular bacterium belonging to the Chlamydiaceae family. Poultry considered be major reservoir of this agent, which has worldwide distribution and a particularly consistent occurrence in chicken flocks. The been linked respiratory disease humans but without definitive confirmation; nevertheless, while it not proved cause human disease, recent report from Italy verified its bird-to-human transmission. This aspect being significant for public health, more...
The present study included three Aeromonas sp. strains isolated from fish tissues during Motile Infection/Motile Septicaemia disease outbreaks on commercial farms, i.e.: hydrophila Pt679 obtained rainbow trout as well popoffii A4 (formerly encheleia) and sobria K928 both carp, which were classified into the new provisional PGO1 serogroup prevailing among aeromonads in Polish aquaculture. structure of O-specific polysaccharides has been previously established. Here, immunochemical studies...
To study the patterns of development body's immune defenses against viruses and pathogenic bacteria, a very diverse range mathematical models has been developed. The well-known antiviral response model by Marchuk Petrov, which describes mechanisms protection cellular humoral types, is based on assumption that environment «organism» homogeneous all process components are instantly mixed.The article summarizes Petrov in order to take into account small spatially distributed diffusion effects...
Bacteria of the Bacillus cereus group are Gram-positive rods and widespread in nature, but little information is currently available about their presence reptiles. Here, we report draft genome sequences six isolates belonging to three species, namely, cereus, paranthracis, toyonensis, isolated from pet reptiles Poland.
Food and waterborne disease (FWD) surveillance requires Whole-Genome Sequencing (WGS)-based systems following a One Health approach. However, different laboratories employ WGS pipelines in their routine activities, casting doubt on the comparability of results hindering optimal communication at intersectoral international levels. Through collaborative effort involving eleven European institutes across seven countries spanning food, animal human health sectors, we aimed to assess...
Contaminations with cephalosporin-resistant Escherichia coli across the food chain may pose a significant threat to public health because those antimicrobials are critically important in human medicine. The impact of presented data is especially concerning Poland’s role as one leading producers EU. This work aimed characterize genomic contents (n = 36) isolated from retail meat expand official AMR monitoring reported by EFSA. ESBL mechanism was predominant (via blaCTX-M-1 and blaSHV-12),...
Abstract Introduction In Europe, veterinary vaccines are strictly controlled by the Official Medicines Control Laboratories (OMCLs) of General European OMCL Network, coordinated Directorate for Quality & HealthCare. Despite a meticulous verification programme immunological medicinal products (IVMPs), products’ genomic composition has not yet been subject to evaluation in pharmacy. Material and Methods A study was carried out on Poland’s poultry containing infectious bronchitis virus...
Small ruminant lentiviruses (SRLVs) exist as populations of closely related genetic variants, known quasispecies, within an individual host. The privileged way SRLVs transmission in goats is through the ingestion colostrum and milk infected does. Thus, characterization SRLV variants transmitted milk, including epithelial cells (MEC), may provide useful information about evolution SRLVs. Therefore, aim this study was to detect peripheral blood leukocytes (PBLs) naturally with perform single...