Diaphragmatic lymphatic function is mainly sustained by pressure changes in the tissue and serosal cavities during cardiorespiratory cycles. The most peripheral diaphragmatic lymphatics are equipped with muscle cells (LMCs), which exhibit spontaneous contraction, whose molecular machinery still undetermined. Hypothesizing that contraction might involve hyperpolarization-activated cyclic nucleotide-gated (HCN) channels LMCs, specimens, including spontaneously contracting lymphatics, were...

The mechanism through which the stresses developed in diaphragmatic tissue during skeletal muscle contraction sustain local lymphatic function was studied 10 deeply anesthetized, tracheotomized adult Wistar rats whose diaphragm exposed after thoracotomy. To evaluate direct effect of on hydraulic intraluminal pressures (Plymph) and vessel geometry, maximal fibers adjacent to a elicited by injection 9.2 nl 1 M KCl solution among while Plymph recorded micropuncture geometry via stereomicroscopy...

The spontaneous contractility of FITC-dextran-filled lymphatics at the periphery pleural diaphragm was documented for first time “in vivo” in anesthetized Wistar rats. We found that lymphatic segments could be divided into four phenotypes: 1) active, displaying rhythmic contractions (51.8% 197 analyzed sites); 2) stretch-activated, whose contraction triggered by passive distension vessel lumen (4.1%); 3) passive, which displayed a completely (4.5%); and 4) inert, diameter never changed over...

Peripheral rat diaphragmatic lymphatic vessels, endowed with intrinsic spontaneous contractility, were in vivo filled fluorescent dextrans and microspheres subsequently studied ex excised samples. Changes diameter lymph velocity detected, a vessel segment, during smooth muscle contraction upon activation, through electrical whole-field stimulation, of skeletal fibers. During flowed both forward backward, net propulsion 14.1 ± 2.9 μm at an average speed 18.0 3.6 μm/s. Each sustained...

Lymph drainage and propulsion are sustained by an extrinsic mechanism, based on mechanical forces acting from the surrounding tissues against wall of lymphatic vessels, intrinsic mechanism attributable to active spontaneous contractions vessel muscle. Despite being heterogeneous, mechanisms underlying generation share a common biochemical nature thus modulated temperature. In this study, we challenged excised rat diaphragm hindpaw, endowed with spontaneously contracting temperatures 24°C...

Lymphatic vessels are capable of sustaining lymph formation and propulsion via an intrinsic mechanism based on the spontaneous contraction lymphatic muscle in wall collectors. Exposure to a hyper- or hypo-osmolar environment can deeply affect rate therefore alter flow. In this work, we aimed at defining putative receptors underlying such response. Functional experiments were conducted ex vivo rat diaphragmatic specimens containing spontaneously contracting that exposed either solutions....

Lymph formation and propulsion rely on an extrinsic mechanism based the forces that surrounding tissues exert upon vessel wall lumen intrinsic spontaneous, rhythmic contractions of lymphatic muscle layer collecting vessels. The two spontaneous pacemakers described in literature involve chloride-dependent depolarizations (STDs) If-like currents, both giving rise to a variable contraction frequency (fc) vessels functional units (lymphangions). Several stimuli have been shown modulate fc, such...

The lymphatic system drains and propels lymph by extrinsic intrinsic mechanisms. Intrinsic propulsion depends upon spontaneous rhythmic contractions of muscles in the vessel walls is critically affected changes surrounding tissue like osmolarity temperature. Lymphatics diaphragm display a steep change contraction frequency response to temperature, this, turn, affects flow. In present work, we demonstrated an ex vivo diaphragmatic rat model that lymphatics express transient receptor potential...

Aim: The proposal of this study was to evaluate, in vitro, the potential paracrine effect human adipose-derived stem cells (hASCs) promote lymphangiogenesis lymphatic endothelial isolated from rat diaphragmatic vessels. Materials & methods: ELISA on VEGFA, VEGFC and IL6 hASC-conditioned medium; LYVE1 immunostaining; gene expression PROX1, VEGFR3, VEGFC, VEGFA were methods used. Results: In 2D culture, medium able cell survival, maintenance cobblestone morphology induction form a vessel-like...

Background: Lymphatic vessels drain fluids and solutes from interstitial spaces serosal cavities. Among the solutes, low-density lipoproteins (LDL) are drained can be detected in peripheral lymph, where they have been reported to exert a modulatory action on lymphatic intrinsic contraction rate. In present work, we investigated vessel mechanical properties (contraction frequency amplitude) that may modulated by LDL application consequence lymph flow. Methods Results: Human-derived were...

Purpose: To investigate the regional gravity-dependent impact of mechanical ventilation and fluid overload on lung extracellular matrix (ECM) in healthy lungs. Materials Methods: The glycosaminoglycans (GAGs) composition ventral dorsal parenchyma was determined anesthetized supine rats mechanically ventilated for 4 hours air: (a) at low (∼7.5 mL/kg) or high (∼ 23 mL /kg) tidal volume (VT) 0 cmH2O positive end-expiratory pressure (PEEP); (b) VT 5 PEEP (c) with without 7 /(kg·h) intravenous...

The present study reports the on-field screening of a population young soccer players in pursuit alterations gait using portable and low-cost analysis system composed Wii Balance Board webcam. Recordings motion lower extremities along with vertical ground reaction force (GRF) were used to quantify coefficients symmetry for overall GRF forces exerted by quadriceps femori acting on anterior cruciate ligament (ACL). Data show that, face quite homogeneous during left right stance phases gait,...

Pacemaking in spontaneously contracting lymphatics is thought to depend upon: a) small transient depolarisations sustained by an inward current through calcium‐dependent chloride channels, seen mesenteric lymphatic vessels of guinea pigs, and, b) a current, observed only bovine and sheep lymphatics, resembling cardiac I f . This study aimed at performing, molecular, functional, immuno‐hystological verify the potential involvement hyperpolarization cyclic nucleotide gated (HCN) channels...

Mechanical ventilation with or without positive pressure in the airways (PEEP) and mild fluid load can cause some modification on morphology of lung matrix ventral dorsal regions supine healthy rats. To evaluate differences between areas lungs after different strategies we subdivided rats two sets animals, one any intravenous infusion, other infusion phosphate buffered saline (PBS) maintained during all mechanical ventilation. Each set was further groups which underwent strategies, vary- ing...

Lymph formation and progression in initial lymphatics is a complex, not completely unveiled process. To clarify it, we studied “in situ” the kinetics of lymph flow diaphragmatic submesothelial network anesthetized, mechanical ventilated rats. In single lymphatic vessel, intraluminar hydraulic pressure (P ) were measured by means micropuncture technique combined with injection fluorescent dextrans to visualise flow. Measurements front vessels diameter performed on acquired images calculate (F...