A5049851677- Ion channel regulation and function
- Aquaculture Nutrition and Growth
- RNA modifications and cancer
- Amino Acid Enzymes and Metabolism
- RNA Research and Splicing
- MicroRNA in disease regulation
- Neuroscience and Neuropharmacology Research
- Cancer-related molecular mechanisms research
- Congenital Heart Disease Studies
- Biochemical Analysis and Sensing Techniques
- Neurobiology and Insect Physiology Research
- Insect Resistance and Genetics
- Peptidase Inhibition and Analysis
- Cancer, Hypoxia, and Metabolism
- Neurological disorders and treatments
- DNA and Nucleic Acid Chemistry
- Ubiquitin and proteasome pathways
- Ion Transport and Channel Regulation
- Lipid Membrane Structure and Behavior
- Parkinson's Disease Mechanisms and Treatments
- Olfactory and Sensory Function Studies
- Physiological and biochemical adaptations
- Congenital heart defects research
- Epigenetics and DNA Methylation
- Trace Elements in Health
University of Insubria
2013-2024
Institute of Molecular Science and Technologies
2010
A recent body of evidence indicates an active role for stromal (mis)-regulation in the progression neoplasias. Within this conceptual framework, genes belonging to growing but still poorly characterized class tumor antagonizing/malignancy suppressor (TAG/MSG) seem play a crucial regulation cross-talk between and epithelial cells by controlling malignant growth vivo without affecting any cancer-related phenotype vitro. Here, we have functionally human RNASET2 gene, which encodes first member...
Total anomalous pulmonary venous return (TAPVR) is a congenital heart defect in which the veins fail to enter left atrium and drain instead into right or one of its tributaries. Although genetic basis for TAPVR has long been recognized, no single gene involved pathogenesis this disease identified date. We previously reported patient bearing de novo 10;21 balanced translocation. In work, we cloned both translocation breakpoints from mapped ANKRD1 gene, encoding cardiac transcriptional...
The region 6q27 from human chromosome 6 has been reported to contain one or more tumor suppressor genes on the basis of cytogenetic, molecular and functional studies. We have recently carried out a detailed analysis candidate gene evaluate its putative role as involved in ovarian cancer pathogenesis. RNASET2 was shown behave class II abolish tumorigenic potential an cancer-derived cell line. In this study, we started cellular biochemical characterization showed that it is secreted...
Chronic rhinosinusitis (CRS) is a frequent disease with high social impact and multifactorial pathogenesis. Recently, single nucleotide polymorphisms within the TAS2R38 gene have been implicated as possible contributors to complex gene-environment interactions in CRS. The purpose of this study was confirm proposed correlation between genotype, CRS related comorbidities. Fifty-three patients 39 healthy individuals were genotyped at locus. treated by endoscopic sinus surgery medical therapies...
The Nramp (Slc11) protein family is widespread in bacteria and eucaryotes, mediates transport of divalent metals across cellular membranes. social amoeba Dictyostelium discoideum harbours two proteins. Nramp1, like its mammalian ortholog, recruited to phagosomal macropinosomal membranes, confers resistance pathogenic bacteria. Nramp2 located exclusively the contractile vacuole membrane controls, synergistically with iron homeostasis. It has long been debated whether Nramp1 import or export...
Diaphragmatic lymphatic function is mainly sustained by pressure changes in the tissue and serosal cavities during cardiorespiratory cycles. The most peripheral diaphragmatic lymphatics are equipped with muscle cells (LMCs), which exhibit spontaneous contraction, whose molecular machinery still undetermined. Hypothesizing that contraction might involve hyperpolarization-activated cyclic nucleotide-gated (HCN) channels LMCs, specimens, including spontaneously contracting lymphatics, were...
The published circulating miRNA signatures proposed for early-stage non-small cell lung cancer (NSCLC) detection are inconsistent and difficult to replicate. Reproducibility validation of an simple signature NSCLC prerequisites translation clinical application.The serum level miR-223 miR-29c, emerging from studies, respectively, as a highly sensitive specific biomarker NSCLC, was measured with droplet digital PCR (ddPCR) technique in Italian cohort 75 patients stage I-II 111 tumor-free...
Peptide transporter 1 (PepT1, alias Slc15a1) mediates the uptake of dietary di/tripeptides in all vertebrates. However, teleost fish, more than one PepT1-type might function, due to specific whole genome duplication event(s) that occurred during their evolution leading a complex paralogue gene repertoire higher vertebrates (tetrapods).Here, we describe novel di/tripeptide zebrafish (Danio rerio), i.e., peptide 1a (PepT1a; also known as Solute carrier family 15 member a1, Slc15a1a), which is...
The identification of markers for early diagnosis, prognosis, and improvement therapeutic options represents an unmet clinical need to increase survival in Non-Small Cell Lung Cancer (NSCLC), a neoplasm still characterized by very high incidence mortality. Here, we investigated whether proline dehydrogenase (PRODH), mitochondrial flavoenzyme catalyzing the key step degradation, played role NSCLC tumorigenesis. PRODH expression was immunohistochemistry; digital PCR, quantitative...
Increased Ankyrin Repeat Domain 1 (ANKRD1) levels linked to gain of function mutations have been associated total anomalous pulmonary venous return and adult cardiomyopathy occurrence in humans. The link between increased ANKRD1 level cardiac structural functional disease is not understood. To get insight into this problem, we generated a mouse model by overexpressing the myocardium.Ankrd1 expressed non-homogeneously embryonic myocardium, with dynamic nucleo-sarcomeric localization...
The ANKRD1/CARP gene encodes a muscle‐specific protein which has been implicated in transcriptional regulation and myofibrillar assembly. Several features at both the mRNA levels define ANKRD1 as whose expression is tightly regulated, deregulated of this recently associated to human congenital heart disease. It therefore crucial intracellular pathways that regulate protein's steady‐state levels. Here, we show short‐lived are regulated by 26S proteasome. In addition, critical role for...
Peptide transporter 1 (PepT1) mediates the uptake of dietary di-/tripeptides in vertebrates. However, teleost fish gut, more than one PepT1-type might operate, because teleost-specific whole gen(om)e duplication event(s) that occurred during evolution. Here, we describe a novel di-/tripeptide transporter, i.e., Atlantic salmon ( Salmo salar) peptide 1a [PepT1a; or solute carrier family 15 member (Slc15a1a)], which is paralog (77% similarity and 64% identity at amino acid level)...
Abstract We report the expression of recombinant RNASET2, only human member Rh/T2/S family acid ribonucleases, in yeast Pichia pastoris and baculovirus‐insect cell heterologous systems. In both models, yield protein was comparable ranged between 5 mg/L (for a catalytically impaired mutant version RNASET2) 30 for wild‐type protein. Thus, produced rather than system used appears to influence after optimization culture conditions. The found undergo heterogeneous glycosylation systems,...
Abstract The purpose of this study is to investigate the presence nervous fibers and expression TRP channels in samples harvested during decompressive/fusion spine surgeries from patients affected by chronic low back pain (CLBP). aim was understand if members family receptors played a role detection processing painful stimuli, eventually define them as potential targets for CLBP alleviation. Expression transient receptor (TRP) (A1, V1, V2, V4, M8) evaluated different periarticular sites 6...
The ability of the SLC6 family members, insect neutral amino acid cotransporter KAAT1(K+-coupled transporter 1) and its homologous CAATCH1(cation anion activated transporter/channel), to transport D-amino acids has been investigated through heterologous expression in Xenopus laevis oocytes electrophysiological techniques. In presence D-isomers leucine, serine, proline, msKAAT1 generates inward, transport-associated, currents with variable relative potencies, depending on driving ion Na+ or...
To the SLC6 family belong 20 human transporters that utilize sodium electrochemical gradient to move biogenic amines, osmolytes, amino acids and related compounds into cells. They are classified two functional groups, Neurotransmitter (NTT) Nutrient acid (NAT). Here we summarize how since their first cloning in 1998, insect (Lepidopteran) Orthologs of have represented very important tools for investigating functional-structural relationships, mechanism transport, ion pH dependence substate...
Membrane proteins are involved in different physiological functions and the target of pharmaceutical abuse drugs. Xenopus laevis oocytes provide a powerful heterologous expression system for functional studies these proteins. Typical experiments investigate transport using electrophysiology radiolabeled uptake. A two-electrode voltage clamp is suitable only electrogenic proteins, uptake measurements require existence substrates adequate laboratory facilities.Recently, Dictyostelium...