A5024964268- Immunodeficiency and Autoimmune Disorders
- Erythrocyte Function and Pathophysiology
- Blood groups and transfusion
- Chronic Lymphocytic Leukemia Research
- Blood disorders and treatments
- Acute Myeloid Leukemia Research
- Platelet Disorders and Treatments
- Immune Cell Function and Interaction
- Complement system in diseases
- Eosinophilic Disorders and Syndromes
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Lymphoma Diagnosis and Treatment
- Blood properties and coagulation
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Lipid Membrane Structure and Behavior
- Acute Lymphoblastic Leukemia research
- Pancreatic function and diabetes
- Ion Transport and Channel Regulation
- Cellular Mechanics and Interactions
- thermodynamics and calorimetric analyses
- Physiological and biochemical adaptations
- Iron Metabolism and Disorders
- Pneumocystis jirovecii pneumonia detection and treatment
- Systemic Sclerosis and Related Diseases
- Ion channel regulation and function
University of Insubria
2015-2021
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2003-2016
Ospedale Maggiore
2002-2012
Istituti di Ricovero e Cura a Carattere Scientifico
2002-2012
To evaluate the sustained response to low-dose (LD) rituximab in autoimmune hemolytic anemia (AIHA), ex vivo effect on anti-RBC antibody production by mitogen-stimulated direct antiglobulin test (MS-DAT), and vitro dose of drug antibodies.Thirty two patients, 18 warm (W) AIHA 14 cold hemagglutinin disease (CHD), were treated with LD (100 mg fixed ×4 weekly infusions) along a short course oral prednisone. Complete clinical examination, blood counts, markers performed at enrollment month 6,...
The Nramp (Slc11) protein family is widespread in bacteria and eucaryotes, mediates transport of divalent metals across cellular membranes. social amoeba Dictyostelium discoideum harbours two proteins. Nramp1, like its mammalian ortholog, recruited to phagosomal macropinosomal membranes, confers resistance pathogenic bacteria. Nramp2 located exclusively the contractile vacuole membrane controls, synergistically with iron homeostasis. It has long been debated whether Nramp1 import or export...
Summary Synergism between Mycobacterium tuberculosis (M. tuberculosis) and HIV-1 infections was demonstrated in several vitro models clinical studies. Here, we investigated their reciprocal effects on growth chronically HIV-1-infected promonocytic U1 cells acutely infected monocyte-derived macrophages (MDM). Phagocytosis of M. induced expression cells, together with increased TNF-α production. growth, evaluated by competitive PCR, greater MDM compared to uninfected cells. phagocytosis IL-10...
B-chronic lymphocytic leukemia (B-CLL) patients have a high prevalence of autoimmune phenomena, mainly hemolytic anemia (AIHA). Immunoregulatory cytokines play role in the regulation both autoimmunity and leukemic B-cell growth. Mitogen-stimulated direct antiglobulin test (MS-DAT) is recently described able to disclose latent anti-RBC AIHA. We investigated by MS-DAT pattern cytokine production PHA-stimulated whole blood cultures from 69 B-CLL 53 controls. Results showed that IgG values...
Autoimmune phenomena, mainly directed against red blood cells are described in myelodysplastic syndromes (MDS), particularly early MDS, i.e. refractory anemia (RA) and RA with ringed sideroblasts (RARS). Dysregulation of apoptosis immunoregulatory cytokines thought to play a role the pathogenesis MDS.This work was aimed investigate anti-erythroid autoimmunity unstimulated mitogen-stimulated peripheral bone marrow cultures 26 patients MDS (RA RARS), relate its presence apoptotic markers...
Toll-like receptors (TLRs) represent major agents of innate immunity and initiators adaptive immunity. TLR4 TLR9 gene expression was related to the occurrence infections, autoimmunity disease progression in 95 patients with B-chronic lymphocytic leukemia (B-CLL), grouped according stage, therapy known prognostic markers, followed prospectively (median 33.6 months, range 25–50). A retrospective analysis 6.8 years, 6–26) also performed. decreased increased versus controls, former being more...
BACKGROUND Hereditary spherocytosis (HS) is a congenital hemolytic anemia caused by defects in red blood cell (RBC) membrane proteins leading to premature RBC clearance the spleen. The presence of autoantibodies has never been extensively investigated HS. STUDY DESIGN AND METHODS antibody–bound immunoglobulin (Ig)G was 91 consecutive HS patients mitogen‐stimulated direct antiglobulin test (MS‐DAT), sensitive method able magnify latent antibody autoimmunity and related with variables,...
BACKGROUND Low‐risk myelodysplastic syndromes (MDS) show several immunologic abnormalities, including increased frequency of autoimmune manifestations and/or overt diseases, whose prognostic significance still remains controversial. STUDY DESIGN AND METHODS We studied the presence erythroblast antibodies in mitogen‐stimulated bone marrow (BM) cultures 70 patients with early‐stage MDS (refractory anemia and refractory ringed sideroblasts). RESULTS Sixty‐six percent showed positive antibodies,...
The ability of the SLC6 family members, insect neutral amino acid cotransporter KAAT1(K+-coupled transporter 1) and its homologous CAATCH1(cation anion activated transporter/channel), to transport D-amino acids has been investigated through heterologous expression in Xenopus laevis oocytes electrophysiological techniques. In presence D-isomers leucine, serine, proline, msKAAT1 generates inward, transport-associated, currents with variable relative potencies, depending on driving ion Na+ or...
Membrane proteins are involved in different physiological functions and the target of pharmaceutical abuse drugs. Xenopus laevis oocytes provide a powerful heterologous expression system for functional studies these proteins. Typical experiments investigate transport using electrophysiology radiolabeled uptake. A two-electrode voltage clamp is suitable only electrogenic proteins, uptake measurements require existence substrates adequate laboratory facilities.Recently, Dictyostelium...