A5046068646- Adipokines, Inflammation, and Metabolic Diseases
- Adipose Tissue and Metabolism
- Natural Antidiabetic Agents Studies
- Coenzyme Q10 studies and effects
- Antioxidant Activity and Oxidative Stress
- Metabolism, Diabetes, and Cancer
- Phytochemicals and Antioxidant Activities
- Atherosclerosis and Cardiovascular Diseases
- HIV-related health complications and treatments
- Immune Cell Function and Interaction
- Vitamin C and Antioxidants Research
- Diet and metabolism studies
- Genomics, phytochemicals, and oxidative stress
- HIV/AIDS Research and Interventions
- Diabetes Treatment and Management
- Tea Polyphenols and Effects
- Advanced Glycation End Products research
- Advanced battery technologies research
- Cardiac Ischemia and Reperfusion
- Pharmacological Effects of Natural Compounds
- Cardiovascular Disease and Adiposity
- Biochemical Acid Research Studies
- Tryptophan and brain disorders
- Inflammatory Biomarkers in Disease Prognosis
- Liver Disease Diagnosis and Treatment
South African Medical Research Council
2016-2025
University of Zululand
2011-2025
University of Limpopo
2024
Marche Polytechnic University
2018-2022
National Research Foundation
2020
University of KwaZulu-Natal
2020
Weatherford College
2020
Stellenbosch University
2016-2018
Lipid overload or metabolic stress has gained popularity in research that explore pathological mechanisms may drive enhanced oxidative myocardial damage. Here, H9c2 cardiomyoblasts were exposed to various doses of palmitic acid (0.06 1 mM) for either 4 24 hours study its potential physiological response cardiac cells. Briefly, assays performed included activity, cholesterol content, mitochondrial respiration, and prominent markers stress, as well determining changes potential, production...
Dyslipidemia is a prominent pathological feature responsible for oxidative stress-induced cardiac damage. Due to their high antioxidant content, dietary compounds, such as aspalathin and sulforaphane, are increasingly explored cardioprotective effects against lipid-induced toxicity. Cultured H9c2 cardiomyoblasts, an in vitro model routinely used assess the pharmacological effect of drugs, were pretreated with (1 μM) sulforaphane (10 before exposure palmitic acid (0.25 mM) induce...
Dimethyl sulfoxide (DMSO) is an effective solvent and cytoprotectant agent that can induce diverse actions in experimental settings, ranging from metabolic stress to cytotoxic effects depending on the concentration used. Therefore, for quality of experiments reproducibility results it essential establish a precise non-toxic dose DMSO within specific cell system. 3T3-L1 adipocytes, represent well-established vitro model used assess anti-obesity potential extracts compounds. Although commonly...
Aspalathin (ASP) can protect H9c2 cardiomyocytes against high glucose (HG)-induced shifts in myocardial substrate preference, oxidative stress, and apoptosis. The protective mechanism of ASP remains unknown. However, as one possible, it is well known that phytochemical flavonoids reduce stress via nuclear factor (erythroid-derived 2)-like 2 (Nrf2) activation resulting up-regulation antioxidant genes enzymes. Therefore, we hypothesized protects the myocardium HG- hyperglycemia-induced damage...
Energy deprivation in the myocardium is associated with impaired heart function. This study aims to investigate if aspalathin (ASP) can ameliorate hyperglycemic-induced shift substrate preference and protect against cell apoptosis.H9c2 cells were exposed to, either normal (5.5 mM) or high (33 glucose concentrations for 48 h. Thereafter, 33 mM treated metformin (1 μM) ASP μM), as well a combination of 6 In vitro studies revealed that improved metabolism by decreasing fatty acid uptake...
We previously demonstrated that an aspalathin-enriched green rooibos extract (GRE) reversed palmitate-induced insulin resistance in C2C12 skeletal muscle and 3T3-L1 fat cells by modulating key effectors of signalling such as phosphatidylinositol-4,5-bisphosphate 3-kinase/protein kinase B (PI3K/AKT) AMP-activated protein (AMPK). However, the effect GRE on hepatic is unknown. The effects lipid-induced using palmitate-exposed C3A liver obese resistant (OBIR) rats were explored. attenuated...