A5090949072- Growth Hormone and Insulin-like Growth Factors
- Neuroendocrine regulation and behavior
- Rabbits: Nutrition, Reproduction, Health
- Animal Nutrition and Physiology
- Hypothalamic control of reproductive hormones
- Amyotrophic Lateral Sclerosis Research
- Meat and Animal Product Quality
- Stress Responses and Cortisol
- Neuroscience of respiration and sleep
- Birth, Development, and Health
- Infant Health and Development
- Regulation of Appetite and Obesity
- Diet and metabolism studies
- Pituitary Gland Disorders and Treatments
- Nerve injury and regeneration
- Pluripotent Stem Cells Research
- Effects of Environmental Stressors on Livestock
- Neurogenetic and Muscular Disorders Research
- Circadian rhythm and melatonin
- Mitochondrial Function and Pathology
- Neonatal Respiratory Health Research
- Ion channel regulation and function
- Mesenchymal stem cell research
- Veterinary Medicine and Surgery
- biodegradable polymer synthesis and properties
Universidad de Zaragoza
2014-2025
Instituto de Investigación Sanitaria Aragón
2019-2023
Centro de Investigación Biomédica en Red
2021-2023
Biomedical Research Networking Center on Neurodegenerative Diseases
2021-2023
Centro de Investigación y Tecnología Agroalimentaria de Aragón
2021-2022
Universidad Nacional Autónoma de México
1981-2013
Institut Cochin
2005
Inserm
1976-2005
Institut Pasteur
2002
Centre National de la Recherche Scientifique
2002
We have compared the effectiveness of TRH and a rat hypothalamic PRL-releasing factor (PRF; previously incubated with serum to destroy TRH) in stimulating release PRL into plasma conscious lactating rats when injected before after pituitary had been depleted transformed releasable by 10 min suckling. (1.25 μg) PRF [equivalent 2.5 stalk median eminence (SME) fragments] each caused small increase (38 30 ng, respectively) concentration within nondepleted mothers. The levels then fell quickly....
AbstractIn the present study, we used SOD1 (G93A) mutant transgenic mice as a model of amyotrophic lateral sclerosis (ALS). This is widely laboratory tool to study experimental treatments in vivo for ALS investigate new therapeutic strategies this neurodegenerative disease. Such studies require objective quantification different parameters while develop We have applied battery and specific tests: scoring motor deficits by trained observer, weighing, survival measure, hanging wire test,...
In lactating rats that were separated from their pups for 8 hours, suckling induced a rapid rise in plasma prolactin levels. Correlatively, it depletion dopamine (DA) and serotonin (5-HT) concentrations within the hypothalamus as early 5 min after onset of suckling. This lasted long stimulus was maintained. A parallel increase level metabolite 5-HT, 5-hydroxyindolacetic acid (5-HIAA), observed. Under these conditions, ratio 5-HIAA/5-HT is augmented. good index turnover transmitter. Suckling...
Since NLRP3 inflammasome plays a pivotal role in several neurodegenerative disorders, we hypothesized that levels of components could help diagnosis or prognosis amyotrophic lateral sclerosis (ALS). Gene and protein expression was assayed by RT-PCR Western blot. Spearman's correlation coefficient used to determine the linear transcriptional with longevity throughout disease progression mice models. Kaplan-Meier analysis performed evaluate MCC950 effects (NLRP3 inhibitor) on lifespan SOD1G93A...
ABSTRACT A trial was conducted to research the impact of reducing dietary crude protein (CP) levels from 14.8% 12.6% in finishing diet (90–120 kg body weight) lean pigs, with an essential AA supply (8.8 g Lys, 2.1 Met, 6.7 Thr, 5.5 Ile, and Val, per kilogram feed) intact males females under commercial setting. The productive performances nitrogen (N) balance, blood markers related metabolism, apparent digestibility nutrients, carcass, meat quality were evaluated. This CP restriction did not...
The amount of pituitary PRL depleted by suckling is known to increase in direct relationship the length previous nonsuckling interval. Therefore, we examined extent which interval influenced plasma concentration attained during prolonged suckling, metabolic clearance rate continuously infused rat PRL, and secretion into circulation. Neither plateau level nor were altered increasing from 2 4 8 h. rates also similar (554, 530, 537 ng/min for 2-, 4-, 8-h nonsuckled groups, respectively)....
We have compared the effectiveness of stalk-median eminence (SME) extracts, dopamine, and bromocriptine (CB-154) in inhibiting release PRL into plasma during suckling when injected before depletion with that injections were given after pituitary stores had been depleted transformed by 10 min suckling. found a single injection either neutralized acidic extracts SME at dose one or two equivalents per rat 625 ng dopamine effectively prevented both within subsequent rise 1-3 onset Likewise, (0.5...
During postnatal growth and after muscle injury, satellite cells proliferate differentiate into myotubes to form repair musculature. Comparison of studies on cell proliferation differentiation characteristics is confounded by the heterogeneity experimental conditions used. To examine influence sex, age, fiber-type origin in vitro properties derived from muscles, fast extensor digitorum longus (EDL) slow soleus (SOL) muscles were extracted male female mice 1 week 3 months age. Myoblast...
Amyotrophic Lateral Sclerosis (ALS) is a paralyzing disorder that kills individuals within three to five years of onset without any possibility for effective treatment. One proposed therapy has been the use neurotrophic factors inhibit apoptosis motorneurones. At present, one way deliver after intramuscular injection motor neurones through adenoviral vectors. An alternative strategy atoxic C fragment tetanus toxin (TTC) as factor carrier motorneurones.We have produced recombinant protein...
Two and 30 min of suckling by 6 pups following 8 hr nonsuckling on postpartum day 7 induced equivalent falls in pituitary prolactin concentration the lactating rat. Neither 2 nor a fall if mothers were previously nonsuckled for 16 hr. Increasing number to 9 at time likewise was ineffective. Milk ejection, however, normal all rats after nonnursing. Normal reductions response occurred providing either 1 or short periods stimulation (5 min) provided mammary glands during first 16-hr isolation...
During development and also in adulthood, synaptic connections are modulated by neuronal activity. To follow such modifications vivo, new genetic tools designed. The nontoxic C-terminal fragment of tetanus toxin (TTC) fused to a reporter gene as LacZ retains the retrograde transsynaptic transport abilities holotoxin itself. In this work, hybrid protein is injected intramuscularly analyze vivo mechanisms intracellular transneuronal traffics at neuromuscular junction (NMJ). Traffic on both...
The effect of ether inhalation upon plasma PRL levels was assessed in lactating rats whose pituitary stores the hormone first had been depleted by a short term (10-min) period suckling comparison with those not prior suckling. Ether, unlike or handling-decapitation stress, did deplete and caused only small increase (36 to 52 ng/ml) concentration hormone. However, when given 10, 60, 120 min after subsided from 10-min suckling, each instance rose within 5--10 200--250 ng/ml sustained at that...
Since biological membranes are composed of lipids and proteins we tested the in vitro antioxidant properties several indoleamines from tryptophan metabolic pathway pineal gland against oxidative damage to synaptosomes isolated rat brain. Free radicals were generated by incubation with 0.1 mM FeCl3, ascorbic acid. Levels malondialdehyde (MDA) plus 4-hydroxyalkenal (4-HDA), carbonyl content measured as indices proteins, respectively. Pinoline was most powerful evaluated, melatonin,...
SUMMARY The results of experiments in which the prolactin primiparous rat pituitary was bioassayed suggested that failure suckling to release after 8 h non-suckling on day 21 post-partum due fact had been discharged from during 8-h period, presumably by exteroceptive signals emanating general environment animal room. This substantiated other assessed indirectly through its stimulatory effects upon milk secretion. In these experiments, mammary glands rats maintained continuously room filled...
A 50–70% depletion in the anterior pituitary concentration of PRL occurred within 5 min electrical stimulation a single abdominal mammary nerve urethane-anesthetized lactating rats previously nonsuckled for 6–8 h. The stimulus parameters were: 1-msec pulses, 10–20/sec at 5–30 V applied sec on and 10 off. remained low another 15–30 after depletion, then repleted to prestimulus levels by 90th min. same pattern depletion-repletion when was as it 180 In other experiments, plasma rose swiftly...