A5028601680- Neuroscience and Neural Engineering
- Neural dynamics and brain function
- SARS-CoV-2 and COVID-19 Research
- Photoreceptor and optogenetics research
- SARS-CoV-2 detection and testing
- Williams Syndrome Research
- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- Neuroinflammation and Neurodegeneration Mechanisms
- Biosensors and Analytical Detection
- Neuroscience and Neuropharmacology Research
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- Prion Diseases and Protein Misfolding
- COVID-19 epidemiological studies
- Viral Infections and Immunology Research
- Alzheimer's disease research and treatments
- RNA regulation and disease
- Pluripotent Stem Cells Research
- Viral gastroenteritis research and epidemiology
- Congenital heart defects research
- RNA Research and Splicing
- Parkinson's Disease Mechanisms and Treatments
- Ferroelectric and Negative Capacitance Devices
University of California, Santa Barbara
2017-2024
Neuroscience Institute
2016
Human brain organoids replicate much of the cellular diversity and developmental anatomy human brain. However, physiology neuronal circuits within remains under-explored. With high-density CMOS microelectrode arrays shank electrodes, we captured spontaneous extracellular activity from derived induced pluripotent stem cells. We inferred functional connectivity spike timing, revealing a large number weak connections skeleton significantly fewer strong connections. A benzodiazepine increased...
The COVID-19 pandemic has created massive demand for widespread, distributed tools detecting SARS-CoV-2 genetic material. hurdles to scalable testing include reagent and instrument accessibility, availability of highly trained personnel, large upfront investment.
Williams syndrome (WS) is a neurodevelopmental disorder caused by genomic deletion of ∼28 genes that results in cognitive and behavioral profile marked overall intellectual impairment with relative strength expressive language hypersocial behavior. Advancements protocols for neuron differentiation from induced pluripotent stem cells allowed us to elucidate the molecular circuitry underpinning ontogeny WS. In patient-derived neurons, we determined expression Williams–Beuren critical...
Abstract The COVID-19 pandemic has created massive demand for widespread, distributed tools detecting SARS-CoV-2 genetic material. hurdles to scalable testing include reagent and instrument accessibility, availability of highly-trained personnel, large upfront investment. Here we showcase an orthogonal pipeline call CREST (Cas13-based, Rugged, Equitable, Scalable Testing) that addresses some these hurdles. Specifically, pairs commonplace reliable biochemical methods (PCR) with low-cost...
Mutations in the MAPT gene that encodes tau lead to frontotemporal dementia (FTD) with pathology evident both cerebral neurons and glia. Human organoids (hCOs) from individuals harboring pathogenic mutations can reveal earliest downstream effects on molecular pathways within a developmental context, generating interacting We found hCOs carrying V337M R406W mutations, cholesterol biosynthesis pathway astrocytes was top upregulated set compared isogenic controls by single-cell RNA sequencing...
We report the presence of co-occurring extracellular action potentials (eAPs) from cultured mouse hippocampal neurons among groups planar electrodes on multielectrode arrays (MEAs). The invariant sequences eAPs coactive electrode groups, repeated co-occurrences, and short interelectrode latencies are consistent with potential propagation in unmyelinated axons. Repeated eAP codetection by multiple was widespread all our data records. Codetection confirms they result same neuron allows these...
The reopening of colleges and universities in the US during coronavirus disease 2019 (COVID-19) pandemic is a significant public health challenge. development accessible practical approaches for severe acute respiratory syndrome 2 (SARS-CoV-2) detection college population paramount deploying recurrent surveillance testing as an essential strategy virus detection, containment, mitigation.To determine prevalence SARS-CoV-2 asymptomatic participants university community by using CREST...
The link between single-cell variation and population-level fate choices lacks a mechanistic explanation despite extensive observations of gene expression epigenetic among individual cells. Here, we found that single human embryonic stem cells (hESCs) have different biased differentiation potentials toward either neuroectoderm or mesendoderm depending on their G1 lengths before the onset differentiation. Single-cell in length operates dynamic equilibrium establishes probability distribution...
Human brain organoids replicate much of the cellular diversity and developmental anatomy human brain. However, neurophysiology within remains relatively under-explored. With high-density CMOS microelectrode arrays shank electrodes, we probed broadband three-dimensional activity organoids. From spiking activity, estimated a directed functional connectivity graph neural network which showed large number weak connections enmeshed skeleton significantly fewer strong connections. Increasing...
Abstract The progress of the COVID-19 pandemic profoundly impacts health communities around world, with unique effects on colleges and universities. Here, we examined prevalence SARS-CoV-2 in 1808 asymptomatic individuals a university campus California, compared for first time performance CRISPR- PCR-based assays large-scale virus surveillance. Our study revealed that there were no cases our population May/June 2020. Using same methods, demonstrated substantial shift approximately one month...
Abstract The small caliber of central nervous system (CNS) axons makes routine study axonal physiology relatively difficult. However, while recording extracellular action potentials from neurons cultured on planer multi-electrode arrays (MEAs) we found activity among groups electrodes consistent with potential propagation in single neurons. Action was evident as widespread, repetitive cooccurrence (eAPs) electrodes. These eAPs occurred invariant sequences and inter-electrode latencies that...
The COVID-19 pandemic has taken a devastating human toll worldwide. development of impactful guidelines and measures for controlling the requires continuous widespread testing suspected cases their contacts through accurate, accessible, reliable methods SARS-CoV-2 detection. Here we describe CRISPR-Cas13-based method detection SARS-CoV-2. assay is called CREST (Cas13-based, rugged, equitable, scalable testing), specific, sensitive, highly accessible. As such, may provide low-cost dependable...
Abstract Human brain organoids replicate much of the cellular diversity and developmental anatomy human brain. However, physiological behavior neuronal circuits within remains relatively under-explored. With high-density CMOS microelectrode arrays (26,400 electrodes) shank electrodes (960 electrodes), we probed broadband three-dimensional extracellular field recordings generated by spontaneous activity organoids. These simultaneously captured local potentials (LFPs) single-unit extracted...
Multi-Electrode Arrays (MEAs) have been widely used to record neuronal activities, which could be in the diagnosis of gene defects and drug effects. In this paper, we address problem classifying vitro MEA recordings mouse human cultures from different genotypes, where there is no easy way directly utilize raw sequences as inputs train an end-to-end classification model. While carefully extracting some features by hand partially solve problem, approach suffers obvious drawbacks such...