A5039211560- Endoplasmic Reticulum Stress and Disease
- RNA regulation and disease
- Head and Neck Surgical Oncology
- Autophagy in Disease and Therapy
- Cellular transport and secretion
- Soft Robotics and Applications
- Genetics, Aging, and Longevity in Model Organisms
- Calcium signaling and nucleotide metabolism
- Connective tissue disorders research
- SARS-CoV-2 and COVID-19 Research
- Bone Metabolism and Diseases
- SARS-CoV-2 detection and testing
- Heat shock proteins research
- Vitamin K Research Studies
- Meningioma and schwannoma management
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Pancreatic function and diabetes
- Photoreceptor and optogenetics research
- Sinusitis and nasal conditions
- Genetic and Kidney Cyst Diseases
- Lysosomal Storage Disorders Research
- Lipid metabolism and biosynthesis
- Neural dynamics and brain function
- Cell death mechanisms and regulation
University of California, Santa Barbara
2019-2025
Altos Labs
2022-2024
Telethon Institute Of Genetics And Medicine
2017-2023
Bay Institute
2022-2023
University of Brescia
2019-2021
Azienda Socio Sanitaria Territoriale degli Spedali Civili di Brescia
2016
The double-stranded RNA sensor kinase PKR is one of four integrated stress response (ISR) kinases that phosphorylate the α subunit eukaryotic initiation factor 2 (eIF2α) in to stress. current model activation considers formation back-to-back dimers as a prerequisite for signal propagation. Here we show signaling involves assembly dynamic clusters. clustering driven by ligand binding PKR’s domain and front-to-front interfaces between domains. clusters are discrete, heterogeneous, autonomous...
Abstract The integrated stress response (ISR) is a fundamental signaling network that leverages the cell’s biosynthetic capacity against different stresses to restore homeostasis. However, when homeostasis unattainable, ISR switches drive cell death and eliminate irreparably damaged cells. Previous work has shown persistent activity of kinase PERK during unyielding endoplasmic reticulum (ER) induces apoptosis downstream receptor 5 (DR5) [1]. ER provides activating signals engage ectodomain...
The endoplasmic reticulum (ER) is a single-copy organelle that cannot be generated de novo, suggesting coordination between the mechanisms overseeing ER integrity and those controlling cell cycle to maintain inheritance. Unfolded Protein Response (UPR) conserved signaling network regulates homeostasis. Here, we show pharmacological genetic inhibition of UPR sensors IRE1, ATF6, PERK in unstressed cells delays cycle, with showing most penetrant effect, which was associated slowdown G
The reopening of colleges and universities in the US during coronavirus disease 2019 (COVID-19) pandemic is a significant public health challenge. development accessible practical approaches for severe acute respiratory syndrome 2 (SARS-CoV-2) detection college population paramount deploying recurrent surveillance testing as an essential strategy virus detection, containment, mitigation.To determine prevalence SARS-CoV-2 asymptomatic participants university community by using CREST...
Abstract The progress of the COVID-19 pandemic profoundly impacts health communities around world, with unique effects on colleges and universities. Here, we examined prevalence SARS-CoV-2 in 1808 asymptomatic individuals a university campus California, compared for first time performance CRISPR- PCR-based assays large-scale virus surveillance. Our study revealed that there were no cases our population May/June 2020. Using same methods, demonstrated substantial shift approximately one month...
Only preclinical studies and case reports have described robotic surgery for endoscopic transnasal skull base surgery.To evaluate the role of a novel endoscope holder, developed transsphenoidal surgery.Patients were prospectively enrolled 3 mo at Neurosurgery Unit Brescia. Endoscope Robot® was used to assist during sphenoidal phase approach, tumor removal, reconstruction. A Likert scale questionnaire given all surgeons after each procedure. Patients who underwent robotic-assisted matched...
Abstract The Integrated Stress Response (ISR) is a conserved signaling network that detects cellular damage and computes adaptive or terminal outcomes. Understanding the mechanisms underly these computations has been difficult because natural stress inputs activate multiple parallel pathways classical ISR inducers have pleiotropic effects. To overcome this challenge, we engineered photo-switchable control over sensor kinase PKR (opto-PKR), which allows virtual of ISR. Using controlled light...
Abstract The skeletal dysplasia spondyloepiphyseal tarda (SEDT) is caused by mutations in the TRAPPC2 gene, which encodes Sedlin, a component of trafficking protein particle (TRAPP) complex that we have shown previously to be required for export type II collagen (Col2) from endoplasmic reticulum. No vertebrate model SEDT has been generated thus far. To address this gap, Sedlin knockout animal mutating orthologous gene ( olSedl ) Oryzias latipes (medaka) fish. OlSedl deficiency leads...
<title>Abstract</title> The integrated stress response (ISR) is a central signaling network that leverages the cell’s biosynthetic capacity against different stresses to restore homeostasis. However, when homeostasis unattainable, ISR switches drive cell death and eliminate irreparably damaged cells. During persistent endoplasmic reticulum (ER) stress, prolonged activity of kinase PERK induces apoptosis downstream receptor 5 (DR5). ER provides activating signals engage ectodomain (ED) DR5...
The TRAnsport-Protein-Particle (TRAPP) complex controls multiple membrane trafficking steps and is thus strategically positioned to mediate cell adaptation diverse environmental conditions, including acute stress. We have identified TRAPP as a key component of branch the integrated stress response that impinges on early secretory pathway. associates with drives recruitment COPII coat granules (SGs) leading vesiculation Golgi an arrest ER export. Interestingly, relocation SGs only occurs in...
Abstract The double-stranded RNA sensor kinase PKR is one of four integrated stress response (ISR) kinases that phosphorylate the alpha subunit eukaryotic initiation factor 2 (eIF2α) in to stress. current model activation considers formation back-to-back dimers as a prerequisite for signal propagation. Here we show signaling involves assembly dynamic clusters. clustering driven by ligand binding PKR’s domain and front-to-front interfaces between domains. clusters are discrete, heterogeneous,...
Symmetric cell division requires partitioning of organelles to daughter cells after mitosis. Upon mitosis, found in multiple copies the are equally distributed cells. Single organelles, such as endoplasmic reticulum (ER) and Golgi apparatus, fragmented prior their re‐distribution We hypothesize that mammalian monitor health ER commitment division. Our goal is define roles unfolded protein response (UPR), a main mechanism maintaining health, during cycle progression. data suggest most...
Abstract The integrated stress response (ISR) is a fundamental signaling network that reprograms the transcriptome and proteome to leverage cell’s biosynthetic capacity against different stresses. Signaling plasticity enabled by distinct ISR sensor kinases detect specific perturbations. dichotomous, with tailored homeostatic outputs terminal one engaged upon overwhelming stress. Through chemical-genetics approach uncouples natural inputs from actuation, we show engages an input-agnostic,...
Abstract The skeletal dysplasia spondyloepiphyseal tarda (SEDT) is caused by mutations in the TRAPPC2 gene, which encodes Sedlin, a component of trafficking protein particle (TRAPP) complex that we have shown previously to be required for export type II collagen (Col2) from endoplasmic reticulum. No vertebrate model SEDT has been generated thus far. To address this gap, Sedlin knockout animal mutating orthologous gene ( olSedl ) Oryzias latipes (medaka) fish. OlSedl deficiency leads...