A5012228892- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- RNA regulation and disease
- Signaling Pathways in Disease
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- SARS-CoV-2 and COVID-19 Research
- Heat shock proteins research
- Fuel Cells and Related Materials
- Phagocytosis and Immune Regulation
- Cell death mechanisms and regulation
University of California, Santa Barbara
2021-2025
University of California, Davis
2016-2017
The double-stranded RNA sensor kinase PKR is one of four integrated stress response (ISR) kinases that phosphorylate the α subunit eukaryotic initiation factor 2 (eIF2α) in to stress. current model activation considers formation back-to-back dimers as a prerequisite for signal propagation. Here we show signaling involves assembly dynamic clusters. clustering driven by ligand binding PKR’s domain and front-to-front interfaces between domains. clusters are discrete, heterogeneous, autonomous...
Abstract The integrated stress response (ISR) is a fundamental signaling network that leverages the cell’s biosynthetic capacity against different stresses to restore homeostasis. However, when homeostasis unattainable, ISR switches drive cell death and eliminate irreparably damaged cells. Previous work has shown persistent activity of kinase PERK during unyielding endoplasmic reticulum (ER) induces apoptosis downstream receptor 5 (DR5) [1]. ER provides activating signals engage ectodomain...
Macroautophagy/autophagy is a starvation and stress-induced catabolic process critical for cellular homeostasis adaptation. Several Atg proteins are involved in the formation of autophagosome subsequent degradation cytoplasmic components, termed autophagy flux. Additionally, expression several proteins, particular Atg8, modulated transcriptionally, yet regulatory mechanisms remain poorly understood. Here we demonstrate that AGC kinase Ypk1, target rapamycin-insensitive TORC2 signaling...
Autophagy is a catabolic process that recycles cytoplasmic contents and crucial for cell survival during stress. The target of rapamycin (TOR) kinase regulates autophagy as part two distinct protein complexes, TORC1 TORC2. negatively according to nitrogen availability. In contrast, TORC2 functions positive regulator amino acid starvation, via its Ypk1, by repressing the activity calcium-dependent phosphatase calcineurin promoting general control (GAAC) response. Precisely how TORC2-Ypk1...
The target of rapamycin (TOR) kinase is a conserved regulator cell growth and functions within 2 different protein complexes, TORC1 TORC2, where TORC2 positively controls macroautophagy/autophagy during amino acid starvation. Under these conditions, signaling inhibits the activity calcium-regulated phosphatase calcineurin promotes general control (GAAC) response autophagy. Here we demonstrate that regulates by controlling respiratory mitochondria. In particular, find mitochondrial oxidative...
<title>Abstract</title> The integrated stress response (ISR) is a central signaling network that leverages the cell’s biosynthetic capacity against different stresses to restore homeostasis. However, when homeostasis unattainable, ISR switches drive cell death and eliminate irreparably damaged cells. During persistent endoplasmic reticulum (ER) stress, prolonged activity of kinase PERK induces apoptosis downstream receptor 5 (DR5). ER provides activating signals engage ectodomain (ED) DR5...
Abstract The double-stranded RNA sensor kinase PKR is one of four integrated stress response (ISR) kinases that phosphorylate the alpha subunit eukaryotic initiation factor 2 (eIF2α) in to stress. current model activation considers formation back-to-back dimers as a prerequisite for signal propagation. Here we show signaling involves assembly dynamic clusters. clustering driven by ligand binding PKR’s domain and front-to-front interfaces between domains. clusters are discrete, heterogeneous,...
Abstract The integrated stress response (ISR) is a fundamental signaling network that reprograms the transcriptome and proteome to leverage cell’s biosynthetic capacity against different stresses. Signaling plasticity enabled by distinct ISR sensor kinases detect specific perturbations. dichotomous, with tailored homeostatic outputs terminal one engaged upon overwhelming stress. Through chemical-genetics approach uncouples natural inputs from actuation, we show engages an input-agnostic,...