A5079315667- Bone health and osteoporosis research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Bone Metabolism and Diseases
- Bone health and treatments
- Molecular Biology Techniques and Applications
- Alkaline Phosphatase Research Studies
- Parathyroid Disorders and Treatments
- Heterotopic Ossification and Related Conditions
- Spectroscopy and Chemometric Analyses
- Infrared Thermography in Medicine
- Optical Imaging and Spectroscopy Techniques
- Bone fractures and treatments
- Bone Tissue Engineering Materials
- Digital Imaging in Medicine
- Laser Applications in Dentistry and Medicine
- Veterinary Orthopedics and Neurology
- Dental Implant Techniques and Outcomes
- Spine and Intervertebral Disc Pathology
- Forensic and Genetic Research
- Essential Oils and Antimicrobial Activity
- Bone and Joint Diseases
- Multiple Myeloma Research and Treatments
- Hip and Femur Fractures
- Medical Imaging and Analysis
- Bone and Dental Protein Studies
VA Tennessee Valley Healthcare System
2011-2017
Vanderbilt University
2012-2017
Vanderbilt University Medical Center
2014-2017
United States Department of Veterans Affairs
2013
ABSTRACT Fracture risk does not solely depend on strength but also fracture toughness; ie, the ability of bone material to resist crack initiation and propagation. Because resistance growth largely depends properties at tissue level, including collagen characteristics, current X-ray based assessment tools may be suitable identify age-related, disease-related, or treatment-related changes in toughness. To useful clinical surrogates that could improve resistance, we investigated potential 1H...
There is potential for Raman spectroscopy (RS) to complement tools bone diagnosis due its ability assess compositional and organizational characteristics of both collagen mineral. To aid this potential, the present study assessed specificity RS peaks composition bone, a birefringent material, different degrees instrument polarization. Specifically, relative changes in were quantified as incident light rotated orientation osteonal interstitial tissue, acquired from cadaveric femurs. In highly...
A decline in the inherent quality of bone tissue is a † Equal contributors contributor to age-related increase fracture risk. Although this well-known, important biochemical factors have yet be identified using Raman spectroscopy (RS), nondestructive, inelastic light-scattering technique. To identify potential RS predictors risk, we applied principal component analysis (PCA) 558 spectra (370–1720 cm –1 ) human cortical acquired from 62 female and male donors (nine each) spanning adulthood...
Raman spectroscopy (RS) has been extensively used to characterize bone composition. However, the link between biomechanics and RS measures is not well established. Here, we leveraged sensitivity of polarization organization, thereby assessing whether can explain differences in toughness genetic mouse models for which traditional peak ratios are informative. In selected mutant mice - activating transcription factor 4 (ATF4) or matrix metalloproteinase 9 (MMP9) knock-outs reduced but strength...
Abstract Background and Objective Laser preconditioning augments incisional wound healing by reducing scar tissue increasing maximum tensile load of the healed [Wilmink et al. (2009) J Invest Dermatol 129(1): 205–216]. Recent studies have optimized treatments or confirmed results using HSP70 as a biomarker. Under hypothesis that plays role in reported to better understand downstream effects laser preconditioning, this study utilized probe‐based Raman spectroscopy (RS) system achieve an vivo...
Polarization Raman Spectroscopy has been used to demonstrate microstructural features and collagen fiber orientation in human mouse bone, concurrently measuring both organization composition; however, it is unclear as what extent these measurements explain the mechanical quality of bone. In a cohort age gender matched cadaveric cortical bone samples (23-101 yr.), we show homogeneity composition structure are associated with related decrease fracture toughness. 64 were machined into uniform...
Activation Transcription Factor 4 (Atf-4) is essential for osteoblast maturation and proper collagen synthesis. We recently found that these bones demonstrate a rare brittleness phenotype, which independent of bone strength. utilized confocal Renishaw Raman microscope (50x objective; NA=.75) to evaluate embedded, polished cross-sections mouse tibia from both wild-type knockout mice at 8 weeks age (24 mice, n<=8 per group). Analysis peak ratios indicated statistically significant changes in...