Cell therapy utilizing mesenchymal stromal cells (MSCs) through paracrine mechanisms holds promise for regenerative purposes. Peritoneal fibrosis (PF) is a significant complication of peritoneal dialysis. Various strategies have been proposed to protect the membrane (PM). This study explores effectiveness adipose-tissue-derived stem (ASCs) and extracellular vesicles (EVs) at mitigating PF using rat model induced by chlorhexidine gluconate. ASC EV treatments effectively prevented an increase...

Immune escape and metastasis are the hallmarks of several types cancer including bladder cancer. One mechanisms involved in these processes has been linked to indoleamine 2,3-dioxygenase (IDO). Although IDO is classically recognized for its immunomodulatory property, it presented nonimmunological effects some tumors. TGF-β1 believed contribute carcinoma development by modulating immunossupressive molecules, IDO. In addition, induces epithelial-mesenchymal transition (EMT), which a critical...

Indoleamine 2, 3-dioxygenase (IDO) is an immunomodulatory molecule that has been implicated in several biological processes. Although IDO linked with some renal diseases, its role fibrosis still unclear. Because may be modulated by TGF-β1, a potent fibrogenic molecule, we hypothesized could involved fibrosis, especially acting the TGF-β1-induced tubular epithelial-mesenchymal transition (EMT). We analyzed expression and activity model of fibrogenesis, effect inhibitor 1-methyl-tryptophan...

Abstract Background Indoleamine 2, 3-dioxygenase-1 (IDO1) is a promising target for immunotherapy in bladder cancer (BC). IDO1 breaks-down tryptophan to generate kynurenine derivatives, which may activate the aryl hydrocarbon receptor (AHR). AHR an important carcinogens, but its association with BC progression was unknown. Two inhibitors used clinical trials are 1-methyl-D-tryptophan (MT) and INCB240360. Because MT aromatic hydrocarbon, it be ligand AHR. We hypothesized that could associated...

Women with preeclampsia (PE) form a vulnerable group for vitamin D3 deficiency. Reabsorption of occurs in the proximal tubule after being endocytosed combination DBP (vitamin D binding protein) by megalin/cubilin receptor. Because proteinuria promotes injury and dysfunction, we hypothesized that present PE could promote loss these components into urine. Twenty preeclamptic patients ten normal pregnant women gestational age greater than 20 weeks composed three groups: NC, normotensive control...

The severity of the bladder carcinoma (BC) is directly linked to cell invasion and metastasis. Indoleamine 2,3-dioxygenase-1 (IDO-1) an INF-γ-induced immunomodulating enzyme that has been cancer invasiveness. Because IDO1 variable among tumors, we analyzed its expression in BC using mice models culture. MB49 cells were orthotopically or ectopically inoculated C57Bl6 evaluate by immunohistochemistry. For vitro experiments, INF-γ was evaluated grade-1 (RT4) grade-3 (T24) lines. Invading...

// Janaina Mendes Ferreira 1 , Luiz Henrique Gomes Matheus Renato Vasconcelos Souza de Almeida 3 Petronio Augusto Melo Kátia Ramos Moreira Leite 2 Claudio Bovolenta Murta Joaquim Francisco Claro Cleber Pinto Camacho José Pontes-Júnior and Humberto Dellê Postgraduate Program in Medicine, Universidade Nove Julho, São Paulo, Brazil Laboratory of Medical Investigation, Urology Department, University Paulo School, Divisão Urologia do Centro Saúde Masculina Hospital Brigadeiro, Correspondence to:...

Abstract Background An accurate prediction of progression is critical to define the management bladder cancer (BC). The ectonucleotidases CD39 and CD73 play strategic roles in calibrating purinergic signals via an extracellular balance between ATP adenosine. altered expression these enzymes plays a potential role tumor invasion metastasis, therefore, it has been proposed prognosis solid tumors. In BC this not yet clear. This study aimed evaluate cohort patients with non-muscle-invasive (NMI)...

According to the American Cancer Society, prostate cancer (PCa) has highest incidence and second-leading cause of death in men, behind only lung cancer. Among mechanisms used escape from immune system, cells can utilize immunomodulatory molecules such as indoleamine 2,3-dioxygenase (IDO). IDO catalyzes degradation tryptophan which lead accumulation metabolites, kynurenine, that contribute its immune-suppressive response towards T thus promoting for cells. Although been pointed a protective...

Abstract Background Indoleamine 2,3-dioxygenase-1 (IDO1) is a promising target for immunotherapy in bladder cancer (BC). IDO1 breaks-down tryptophan to generate kynurenine derivates, which may activate the aryl hydrocarbon receptor (AHR). AHR an important carcinogens, but its association with BC progression was unknown. Two inhibitors used clinical trials are 1-methyl-D-tryptophan (MT) and INCB240360. Because MT aromatic hydrocarbon, it be ligand AHR. We hypothesized that could associated...

Chronic kidney disease (CKD) is a progressive and high prevalent worldwide. Its prevalence doubled on USA the last decade as such, deserves attention worry to better know its mechanisms. Renal fibrosis lead event happening in CKD, characterized by an extensive inflammatory process with vast macrophage migration extracelular matrix accumulation, those leading damages architecture renal function, thus worsening patient condition. In this frame, Indoleamine 2,3‐dioxygenase (IDO),...

Recurrence of bladder cancer after resections with progression to more aggressive forms are critical promote cancer‐specific death and metastasis. Subpopulations cells, including stem‐like have been found inside the tumors, having different abilities for malignancy chemotherapy resistance. described isolated from a culture T24 which represent major human epithelial cell clinical relevance. However, in situ influences can not be assessed by another way except animal model. Since cells...

Indoleamine 2,3‐dioxygenase (IDO) is an enzyme with immunomodulatory properties and it expressed in the placental tissue, therefore, has a role protecting embryo against maternal immune system. The expression of IDO been found several types cancer, including bladder being related to tumor development. In addition, vessels, hypothesizing that could be involved tumoral angiogenesis process. aim was verify if influence on process induced by cancer cells underwent hypoxia, analyzing effect...

HOTAIR is a long non‐coding RNA (lncRNA) which implicated in cell programming for initiation of epithelial mesenchymal transition (EMT) several types cancer. In bladder carcinoma T24 cells, the TGF‐beta1 promotes invasion and metastasis through induction EMT, however, there are evidences to believe that possible link between EMT The aim this study was analyze expression IncRNA human cells incubated with TGF‐beta1, order correlate it markers EMT. Bladder (T24) were (5 ng/ml) during 48h then...

The most common form of human bladder cancer is non‐muscle invasive, however, half these cases progress to a muscle‐invasive, which ultimately leads metastasis and cancer‐specific death. Indoleamine 2,3‐dioxygenase (IDO) an enzyme induced strongly by INF‐gamma that has been recognized as immunomodulatory molecule since it was described in the placenta protecting embryos tissue against maternal immune attack. A growing body evidence suggests IDO expressed tumors, enabling cancers evade...

Background Indoleamine 2,3‐dioxygenase (IDO) is an enzyme that acts as immunomodulatory molecule. It found in several cancer types where it seems to be associated with tumor escape due its immunosuppressive mechanisms. Some studies have reported a correlation between carcinogenesis and changes IDO expression. However, the role of expression prostate (PC) unclear. The aim our study was evaluate localized PC, correlate classic prognostic factor recurrence after surgical treatment. Methods For...

Bladder cancer (BC) is the fourth most common between men and ninth in women, with a high recurrence rate progression after conventional treatments. A growing body of evidence suggests that immunomodulatory molecules protect bladder tumors against immune surveillance by triggering signaling pathways modulate locally system. One important molecule indoleamine 2,3‐dioxygenase (IDO), which promotes tryptophan breakdown, activating sensitive such as GCN2 cells. In addition, breakdown leads to...

Abstract Background : Indoleamine 2,3-dioxygenase-1 (IDO1) is a promising target for immunotherapy in bladder cancer (BC). IDO1 breaks-down tryptophan to generate kynurenine derivatives, which may activate the aryl hydrocarbon receptor (AHR). AHR an important carcinogens, but its association with BC progression was unknown. Two inhibitors used clinical trials are 1-methyl-D-tryptophan (MT) and INCB240360. Because MT aromatic hydrocarbon, it be ligand AHR. We hypothesized that could...

Abstract Background : Indoleamine 2,3-dioxygenase-1 (IDO1) is a promising target for immunotherapy in bladder cancer (BC). IDO1 breaks-down tryptophan to generate kynurenine derivatives, which may activate the aryl hydrocarbon receptor (AHR). AHR an important carcinogens, but its association with BC progression was unknown. Two inhibitors used clinical trials are 1-methyl-D-tryptophan (MT) and INCB240360. Because MT aromatic hydrocarbon, it be ligand AHR. We hypothesized that could...