A5049432886- Hematopoietic Stem Cell Transplantation
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Mesenchymal stem cell research
- RNA Interference and Gene Delivery
- Cytomegalovirus and herpesvirus research
- Mast cells and histamine
- Renal Transplantation Outcomes and Treatments
- Acute Myeloid Leukemia Research
- Cell Adhesion Molecules Research
- Transplantation: Methods and Outcomes
- Cancer Cells and Metastasis
- Galectins and Cancer Biology
- Caveolin-1 and cellular processes
- Polyomavirus and related diseases
- Phagocytosis and Immune Regulation
- Urticaria and Related Conditions
- Effects of Radiation Exposure
- Wnt/β-catenin signaling in development and cancer
- Ion Transport and Channel Regulation
- Reproductive System and Pregnancy
- Virus-based gene therapy research
- Xenotransplantation and immune response
Stanford University
2008-2019
Stanford Blood Center
2011
Stanford Medicine
2008-2010
University Hospital Carl Gustav Carus
2004-2007
TU Dresden
2001-2006
Klinik und Poliklinik für Psychotherapie und Psychosomatik
1999-2001
The orphan G protein-coupled receptor (GPCR) GPR124/tumor endothelial marker 5 is highly expressed in central nervous system (CNS) endothelium. Here, we show that complete null or endothelial-specific GPR124 deletion resulted embryonic lethality from CNS-specific angiogenesis arrest forebrain and neural tube. Conversely, overexpression throughout all adult vascular beds produced hyperproliferative malformations. In vivo, functioned cell-autonomously endothelium to regulate sprouting,...
Lag-3 has emerged as an important molecule in T cell biology. We investigated the role of conventional (Tcon) and regulatory (Treg) function murine GVHD with hypothesis that engagement diminishes alloreactive responses after bone marrow transplantation. demonstrate deficient Tcon (Lag-3−/− Tcon) induce significantly more severe than wild type (WT) absence on CD4 but not CD8 cells is responsible for exacerbating GVHD. Lag-3−/− exhibited increased activation proliferation indicated by CFSE...
Prominin/CD133 is a 115/120-kDa integral membrane glycoprotein specifically associated with plasma protrusions in epithelial and non-epithelial cells including neuroepithelial hematopoietic stem cells. Here we report the identification as well molecular cell biological characterization of mouse, rat, human prominin-2, 112-kDa structurally related to prominin (referred prominin-1). Although amino acid identity between prominin-2 prominin-1 low (<30%), their genomic organization strikingly...
Abstract Adoptive transfer of freshly isolated natural occurring CD4+CD25+Foxp3+ regulatory T cells (Treg) prevents graft-versus-host disease (GVHD) in several animal models and following hematopoietic cell transplantation (HCT) clinical trials. Donor-derived Treg have been mainly used, as they share the same MHC with CD4+ CD8+ conventional (Tcon) that are primarily responsible for GVHD. Third party–derived a promising alternative cellular therapy, can be prepared advance, screened pathogens...
Because of the perception that depleting hematopoietic grafts T cells will result in poorer immune recovery and increased risk graft rejection, pure stem (HSC), which avoid potentially lethal complication graft-versus-host disease (GVHD), have not been used for allogeneic cell transplantation (HCT) humans. Ideal should contain HSC plus mature confer only benefits protection from pathogens suppression malignancies. This goal requires better understanding effects each blood type its...
The adoptive transfer of CD4+CD25+Foxp3+ regulatory T cells (Tregs) in murine models allogeneic hematopoietic cell transplantation (HCT) has been shown to protect recipient mice from lethal acute graft-versus-host disease (GVHD) and this approach is being actively investigated human clinical trials. Here, we examined the effects cryopreservation on Tregs. We found that freeze thaw Tregs associated with reduced expression L-selectin (CD62L), which was previously established be an important...
Graft-versus-host disease (GVHD) remains a major complication of allogeneic hematopoietic cell transplantation. Acute GVHD (aGVHD) results from direct damage by donor T cells, whereas the biology chronic (cGVHD) with its autoimmune-like manifestations poorly understood, mainly because paucity representative preclinical models. We examined over an extended time period 7 MHC-matched, minor antigen-mismatched mouse models for development cGVHD. Development and cGVHD were determined combination...
Impaired immunity is a fundamental obstacle to successful allogeneic hematopoietic cell transplantation. Mature graft T cells are thought provide protection from infections early after transplantation, but can cause life-threatening graft-vs.-host disease. Human CMV major pathogen We studied reactivity against the mouse homologue, murine (MCMV), in lethally irradiated mice given purified stem (HSCs) or HSCs supplemented with T-cell subsets. Unexpectedly, recipients of mounted superior...
Embryonic stem cells (ESCs) hold promise for the treatment of many medical conditions; however, their utility is limited by immune rejection. The objective our study to establish tolerance or promote engraftment transplanted ESCs as well mature cell populations derived from ESCs. Luciferase (luc(+))-expressing were utilized monitor survival and differentiated progeny in living recipients. Allogeneic recipients conditioned with fractioned total lymphoid irradiation (TLI) anti-thymocyte serum...
T cells are widely used to promote engraftment of hematopoietic stem (HSCs) during an allogeneic cell transplantation. Their role in overcoming barriers HSC is thought be particularly critical when patients receive reduced doses preparative chemotherapy and/or radiation compared with standard transplantations. In this study, we sought delineate the effects CD4+ on and blood formation a model that simulates clinical transplantation by transplanting MHC-matched, minor...