A5034559948- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Immune Cell Function and Interaction
- HIV/AIDS Research and Interventions
- Cytomegalovirus and herpesvirus research
- Marine Toxins and Detection Methods
- HIV-related health complications and treatments
- Sulfur Compounds in Biology
- Protist diversity and phylogeny
- Metabolism and Genetic Disorders
- Viral-associated cancers and disorders
- Microbial Community Ecology and Physiology
- SARS-CoV-2 and COVID-19 Research
- Amino Acid Enzymes and Metabolism
- SARS-CoV-2 detection and testing
- Marine and coastal ecosystems
- Mitochondrial Function and Pathology
- Virus-based gene therapy research
- T-cell and B-cell Immunology
- Immunodeficiency and Autoimmune Disorders
- Chronic Lymphocytic Leukemia Research
- Immunotherapy and Immune Responses
- Ubiquitin and proteasome pathways
- Hepatitis B Virus Studies
- Neonatal Health and Biochemistry
University of Urbino
2015-2024
Regione Puglia
2011
Indian Council of Agricultural Research
2010
Luigi Sacco Hospital
2006
University of Milan
2006
GTx (United States)
2006
Institute of Bioorganic Chemistry
1998
University of Genoa
1996-1997
Yale University
1996
Azienda Sanitaria Locale di Asti
1994
Objective: Inadequate CD4 cell count recovery despite full HIV RNA control occurs in 30% of HAART-treated HIV-infected patients. A better understanding the relationship between T-cell dynamics and intracellular reservoir patients failing to recover following long-term HAART, is required. Methods: In a cross-sectional study turnover homeostatic parameters featuring discordant responses were investigated 27 immunologic non-responders (INR; count, ≤ 200 cells/μl; RNA, 50 copies/ml), 15...
Background We describe the development and validation of a new quantitative real time PCR (qrt-PCR) method for enumeration toxic benthic dinoflagellate Ostreopsis cf. ovata in marine environment. The sp. has world-wide distribution is associated during high biomass proliferation with production potent palytoxin-like compounds affecting human health Species-specific identification, which relevant complex different toxins production, by traditional methods microscopy difficult due to...
We report the development and validation of a qPCR based method for estimation toxic benthic dinoflagellate Ostreopsis cf. ovata in complex matrix marine aerosol at Sant Andreu de Llavaneres beach (northwestern Mediterranean Sea). Toxic events humans after inhalation or cutaneous contact have been reported during O. blooms were attributed to palytoxin (PLTX)-like compounds produced by this microalga. Similar PCR efficiencies plasmid cellular environmental standard curves (98 100%,...
The phase II multicenter, randomized, open label, therapeutic trial (ISS T-002, Clinicaltrials.gov NCT00751595) was aimed at evaluating the immunogenicity and safety of biologically active HIV-1 Tat protein administered 7.5 or 30 μg, given 3 5 times monthly, exploring immunological virological disease biomarkers. study duration 48 weeks, however, vaccinees were followed until last enrolled subject reached weeks. Reported are final data up to 144 weeks follow-up. ISS T-002 conducted in 11...
ABSTRACT The size of lentiviral DNA reservoirs reflects the effectiveness immune responses against lentiviruses. So far, abundant information has been gathered on control HIV-1 replication. Understanding innate mechanisms contributing to containment HIV reservoir, however, are only partly clarified and relevant guiding interventions for reservoir or eradication. We studied contribution natural killer (NK) cell functional features in patients controlling replication either spontaneously (HIV...
This study reports some recent phylogeographical considerations on the genus Ostreopsis distribution worldwide, with particular attention to Mediterranean Sea, and new advances quali-quantitiative detection of species along coastal areas Sea based PCR quantitative real time (qrt-PCR) assays. It was found that O. cf. ovata is widely dispersed throughout tropical warm temperate areas. In Atlantic/Mediterranean region it represents a panmictic population highly divergent from Indo-Pacific...
Injection of the LP-BM5 murine leukemia virus into mice causes AIDS, a disease characterized by many dysfunctions immunocompetent cells. To establish whether is glutathione imbalance, reduced (GSH) and cysteine were quantified in different organs. A marked redox consisting GSH and/or depletion, was found lymphoid organs, such as spleen lymph nodes. Moreover, significant decrease levels pancreas brain, respectively, measured at 5 weeks postinfection. The Th2 immune response predominant all...
The humoral response after vaccination was evaluated in 1248 individuals who received different COVID-19 vaccine schedules. study compared subjects primed with adenoviral ChAdOx1-S (ChAd) and boosted BNT162b2 (BNT) mRNA vaccines (ChAd/BNT) to homologous dosing BNT/BNT or ChAd/ChAd vaccines. Serum samples were collected at two, four six months vaccination, anti-Spike IgG responses determined. heterologous induced a more robust immune than the two vaccinations. ChAd/BNT stronger all time...
Background The aim of this study was to investigate the molecular mechanisms involved in production Th1 cytokines, namely IL-12 and IL-27, when intra-macrophage redox state altered by different chemical entities such as GSH-C4, which is reduced glutathione carrying an aliphatic chain, or I-152, a pro-drug N-acetyl-cysteine (NAC) beta-mercaptoethylamine. We had already demonstrated that GSH-C4 I-152 could shift immune response towards Ovalbumin-immunized mice well enhance HIV-1 Tat-immunized...
Although combined antiretroviral therapy (cART) has saved millions of lives, it is incapable full immune reconstitution and virus eradication. The transactivator transcription (Tat) protein a key human immunodeficiency (HIV) virulence factor required for replication transmission. Tat expressed released extracellularly by infected cells also under cART in this form induces dysregulation, promotes reactivation, entry spreading. Of note, anti-Tat antibodies are rare natural infection and, when...
Introduction: Tat, a key HIV virulence protein, has been targeted for the development of therapeutic vaccine aimed at cART intensification. Results from phase II clinical trials in Italy (ISS T-002) and South Africa T-003) cART-treated patients indicated that Tat vaccination promotes return to immune homeostasis reduces virus reservoir. Here we present data 92 vaccinees enrolled ISS T-002 8-year extended follow-up study EF-UP, ClinicalTrials.gov NCT02118168). Results: Anti-Tat antibodies...
The infectivity and replication of human (HIV-1), feline (FIV), murine (LP-BM5) immunodeficiency viruses are all inhibited by several nucleoside analogues after intracellular conversion to their triphosphorylated derivatives. At the cellular level, main problems in use these drugs concern limited phosphorylation some cells (e.g., macrophages) cytotoxic side effects analogue triphosphates. To overcome limitations a new homodinucleotide,...
Mammalian hexokinase type I is a 100 kDa enzyme that has been considered to be evolved from an ancestral 50 yeast-type hexokinase, insensitive product inhibition, by gene duplication and fusion. According this model, based on many experimental data, the catalytic site associated with C-terminal half of enzyme, although allosteric for binding glucose 6-phosphate could present N-terminal molecule. We have isolated cDNA clone lambda gt11 human placenta library comprising 2658 bp, containing...
Background The quantitative measurement of various HIV-1 DNA forms including total, unintegrated and integrated provirus play an increasingly important role in infection monitoring treatment-related research. We report the development validation a SYBR Green real time PCR (TotUFsys platform) for simultaneous quantification total extrachromosomal patients. This innovative technique makes it possible to obtain both measurements single run starting from frozen blood employing same primers...
Current highly active antiretroviral therapy (HAART) cannot eliminate HIV-1 from infected persons, mainly because of the existence refractory viral reservoir(s). Beyond latently-infected CD4+-T lymphocytes, macrophages (M/M) are important persistent reservoirs for HIV in vivo, that represent a major obstacle to eradication. Therefore, rational therapeutic approach directed selective elimination long-living HIV-infected M/M may be relevant infection. Here we report chronic infection human...
Background The bone marrow (BM) cytokine milieu might substantially affect T-lymphocyte homeostasis in HIV-positive individuals. Interleukin-7 (IL-7) is a marrow-derived regulating T-cell through CD4+-driven feedback loop. CD4+ T-lymphopenia associated with increased free IL-7 levels and reduced IL-7R expression/function, which are only partially reverted by highly active antiretroviral therapy (HAART). We investigated the BM production, peripheral expression signaling (pStat5+ Bcl-2+...
The transmission of SARS-CoV-2 occurs through direct contact (person to person) and indirect by means objects surfaces contaminated secretions from individuals with COVID-19 or asymptomatic carriers. In this study, we evaluated the presence RNA on made different materials located in university environments frequented students staff involved academy activity during fourth pandemic wave (December 2021). A total 189 environmental samples were collected classrooms, library, computer room, gym...