A5011872782- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Vanadium and Halogenation Chemistry
- biodegradable polymer synthesis and properties
- Microplastics and Plastic Pollution
- Chemistry and Chemical Engineering
- Electrochemical sensors and biosensors
- Asymmetric Hydrogenation and Catalysis
- Analytical Chemistry and Chromatography
- Microbial Metabolism and Applications
- Metal-Catalyzed Oxygenation Mechanisms
- Synthesis and Catalytic Reactions
- Process Optimization and Integration
- Advanced Control Systems Optimization
- Effects and risks of endocrine disrupting chemicals
- Microbial Natural Products and Biosynthesis
- Pharmacogenetics and Drug Metabolism
- Innovative Microfluidic and Catalytic Techniques Innovation
- Plant biochemistry and biosynthesis
- Carbon dioxide utilization in catalysis
- Enzyme-mediated dye degradation
- Glycosylation and Glycoproteins Research
- Oxidative Organic Chemistry Reactions
- Advanced Nanomaterials in Catalysis
- Viral Infectious Diseases and Gene Expression in Insects
Institute of Molecular Life Sciences
2021-2024
Universität Greifswald
2014-2016
Polyethylene (PE) is the most commonly used plastic type in world, contributing significantly to waste crisis. Microbial degradation of PE natural environments unlikely due its inert saturated carbon‐carbon backbones, which are difficult break down by enzymes, challenging development a biocatalytic recycling method for waste. Here, we demonstrated depolymerization low‐molecular‐weight (LMW) using an enzyme cascade that included catalase‐peroxidase, alcohol dehydrogenase, Baeyer Villiger...
The synthesis of enantiopure amines via amine transaminases involves several challenges including unfavorable reaction equilibria and product inhibition. Described here is a non‐catalytic approach to overcome such problems by using an in situ‐product crystallization (ISPC) selectively remove targeted from transaminase‐catalyzed reaction. continuous removal the its solution as barely soluble salt effectively yields displacement equilibrium towards products facilitates simple downstream...
Abstract Halohydrin dehalogenases (HHDHs) are valuable biocatalysts for the synthesis of β‐substituted alcohols based on their epoxide ring‐opening activity with a number small anionic nucleophiles. In an attempt to further broaden scope substrates accepted by these enzymes, panel 22 HHDHs was investigated in conversion aliphatic and aromatic vicinally di‐substituted trans ‐epoxides using azide as nucleophile. The majority able convert methyl‐substituted corresponding azidoalcohols, some...
Enantioenriched azido alcohols are precursors for valuable chiral aziridines and 1,2-amino alcohols, however their substituted analogues difficult to access. We established a cascade the asymmetric azidohydroxylation of styrene derivatives leading 1,2-azido via enzymatic epoxidation, followed by regioselective azidolysis, affording with up two contiguous stereogenic centers. A newly isolated two-component flavoprotein monooxygenase StyA proved be highly selective epoxidation nicotinamide...
Abstract A chemoenzymatic method for the halocyclization of unsaturated alcohols and acids by using robust V‐dependent chloroperoxidase from Curvularia inaequalis ( Ci VCPO) as catalyst has been developed in situ generation hypohalites. broad range halolactones cyclic haloethers are formed with excellent performance biocatalyst.
Singlet oxygen is a reactive species undesired in living cells but rare and valuable reagent chemical synthesis. We present fluorescence spectroscopic analysis of the singlet-oxygen formation activity commercial peroxidases novel peroxygenases. Singlet-oxygen sensor green (SOSG) used as fluorogenic singlet trap. Establishing kinetic model for reaction cascade to fluorescent SOSG endoperoxide permits enzymatic formation. All peroxygenases show No could be found any catalase under...
A recombinant isoenzyme of pig liver esterase was used for the highly enantioselective desymmetrization dimethyl cyclohex-4-ene-cis-1,2-dicarboxylate. The selected showed a significant advantage in enantioselectivity over commonly from mammalian source. process scaled up to yield 265 g product with simplified pH control, and target molecule obtained an enantiopurity >99.5% ee.
Invited for this month's cover is the group of Prof. Dr. Frank Hollmann at Delft University Technology in Netherlands. The Front Cover shows vanadium-dependent haloperoxidase from marine organism Curcuvaria inaequalis, which efficiently activates halides as hypohalites that can then initiate spontaneous halo-lactonization and halo-etherification reactions. Communication itself available 10.1002/cssc.201902240.
A chemoenzymatic approach has been developed for the preparation of sertraline, an established anti‐depressant drug. Ketoreductases (KREDs) were employed to yield a key chiral precursor. The bioreduction racemic tetralone exhibited excellent enantioselectivity (>99 % ee ) and diastereomeric ratio (99:1) at 29 conversion (the maximum theoretical is 50 %) after 7 hours. resulting ( S , )‐alcohol was efficiently oxidized enantiopure )‐ketone, immediate precursor by using sodium hypochlorite...
In this work, the development of a chemoenzymatic process for production (1S,2R)-1-(methoxycarbonyl)cyclohex-4-ene-2-carboxylic acid by ECS-PLE06 (recombinant pig liver esterase) is presented. Herein an optimized esterification protocol, starting from economically favored meso-anhydride 1b, was established and combined with highly selective ECS-PLE06-catalyzed desymmetrization reaction. Both reactions proceed under mild reaction conditions, excellent selectivity results in enantiomeric...
Previously, the biocatalytic desymmetrization of dimethyl cyclohex-4-ene-cis-1,2-dicarboxylate to (1S,2R)-1-(methoxycarbonyl)cyclohex-4-ene-2-carboxylic acid, an important intermediate toward synthesis biologically active molecules, had been well-characterized in terms pH and temperature optima several aspects process performance. Eventually this promising reaction could convert 200 mM (40 g·L–1) substrate with >99.5% ee using recombinant pig liver esterase, ECS-PLE06, at a scale 8.8 L....
The recombinant pig liver esterase catalyzed hydrolysis of cis-1,4-diacetoxy-2-cyclopentene forming (1S,4R)-4-hydroxy-2-cyclopentenyl acetate was investigated and realized at preparative scale. Relevant reaction conditions were examined optimized to achieve full conversion with an enantiomeric excess about 86% ee. Enantiopure product then obtained after enantioselective crystallization, which required further studies the solid phase behavior, including its binary melting point diagram.
Abstract The efficient multifunctionalization by one‐pot or cascade catalytic systems has developed as an important research field, but is often challenging due to incompatibilities cross‐reactivities of the catalysts leading side product formation. Herein we report stereoselective preparation cis ‐ and trans ‐4‐aminocyclohexanol from potentially bio‐based precursor 1,4‐cyclohexanedione. We identified regio‐ enzymes catalyzing reduction transamination diketone, which can be performed in a...
Multi-step cascade reactions have gained increasing attention in the biocatalysis field recent years. In particular, multi-enzymatic cascades can achieve high molecular complexity without workup of reaction intermediates thanks to enzymes’ intrinsic selectivity; and where enzymes fall short, organo- or metal catalysts further expand range possible synthetic routes. Here, we present two enantiocomplementary (chemo)-enzymatic composed either a styrene monooxygenase (StyAB) Shi epoxidation...
Polyethylene (PE) is the most commonly used plastic type in world, contributing significantly to waste crisis. Microbial degradation of PE natural environments unlikely due its inert saturated carbon‐carbon backbones, which are difficult break down by enzymes, challenging development a biocatalytic recycling method for waste. Here, we demonstrated depolymerization low‐molecular‐weight (LMW) using an enzyme cascade that included catalase‐peroxidase, alcohol dehydrogenase, Baeyer Villiger...