A5026377612- Single-cell and spatial transcriptomics
- Neonatal Respiratory Health Research
- Renal and related cancers
- Pregnancy and preeclampsia studies
- Genetics, Aging, and Longevity in Model Organisms
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Pluripotent Stem Cells Research
- Mesenchymal stem cell research
- Immune cells in cancer
- Neuroendocrine Tumor Research Advances
- Genomics and Chromatin Dynamics
- MicroRNA in disease regulation
- Zebrafish Biomedical Research Applications
- Lung Cancer Research Studies
- Pancreatic and Hepatic Oncology Research
- Cancer-related gene regulation
- Immune responses and vaccinations
- Blood disorders and treatments
- Platelet Disorders and Treatments
- Viral Infections and Outbreaks Research
- Genomic variations and chromosomal abnormalities
- Epigenetics and DNA Methylation
- Liver physiology and pathology
- Myeloproliferative Neoplasms: Diagnosis and Treatment
Boston Medical Center
2020-2024
Boston University
2020-2024
University of Massachusetts Lowell
2019
Age-related changes in immune cell composition and functionality are associated with multimorbidity mortality. However, many centenarians delay the onset of aging-related disease suggesting presence elite immunity that remains highly functional at extreme old age.To identify immune-specific patterns aging human longevity, we analyzed novel single profiles from peripheral blood mononuclear cells (PBMCs) a random sample 7 (mean age 106) publicly available RNA-sequencing (scRNA-seq) datasets...
The human hematopoietic stem cell harbors remarkable regenerative potential that can be harnessed therapeutically. During early development, cells in the fetal liver undergo active expansion while simultaneously retaining robust engraftment capacity, yet underlying molecular program responsible for their efficient remains unclear. Here, we profile 26,407 at both transcriptional and protein level including ~7,000 highly enriched functional to establish a detailed signature of potential....
Aging CellVolume 24, Issue 1 e14479 ADDITIONAL COVEROpen Access Additional Cover Todd W. Dowrey, Dowrey orcid.org/0000-0001-6086-6306 Search for more papers by this authorSamuel F. Cranston, Samuel CranstonSearch authorNicholas Skvir, Nicholas Skvir orcid.org/0000-0002-3067-4458 authorYvonne Lok, Yvonne LokSearch authorBrian Gould, Brian GouldSearch authorBradley Petrowitz, Bradley Petrowitz orcid.org/0009-0006-1039-0972 authorDaniel Villar, Daniel VillarSearch authorJidong Shan, Jidong...
Neuroendocrine tumors (NETs) are rare cancers that most often arise in the gastrointestinal tract and pancreas. The fundamental mechanisms driving gastroenteropancreatic (GEP)-NET growth remain incompletely elucidated; however, heterogeneous clinical behavior of GEP-NETs suggests both cellular lineage dynamics tumor microenvironment influence pathophysiology. Here, we investigated single-cell transcriptomes immune cells from patients with NETs. Malignant GEP-NET expressed genes regulons...
Hemogenic endothelial cells (HECs) are specialized that undergo endothelial-to-hematopoietic transition (EHT) to give rise the earliest precursors of hematopoietic progenitors will eventually sustain hematopoiesis throughout lifetime an organism. Although HECs thought be primarily limited aorta-gonad-mesonephros (AGM) during early development, EHT has been described in various other organs and embryonic vessels. Though not defined as a organ, lung houses many resident cells, aids platelet...
Abstract Centenarians provide a unique lens through which to study longevity, healthy aging, and resiliency. Moreover, models of human aging resilience disease that allow for the testing potential interventions are virtually non‐existent. We obtained characterized over 96 centenarian offspring peripheral blood samples including those connected functional independence data highlighting resistance disability cognitive impairment. Targeted methylation arrays were used in molecular clocks...
Abstract Age-related changes in immune cell composition and functionality are associated with multimorbidity mortality. However, many centenarians delay the onset of aging-related disease suggesting presence elite immunity that remains highly functional at extreme old age. To identify immune-specific patterns aging human longevity, we analyzed novel single profiles from peripheral blood mononuclear cells (PBMCs) 7 (mean age 106) publicly available RNA-sequencing (scRNA-seq) datasets included...
The protocols herein outline the use of qRT-PCR to detect presence SARS-CoV-2 genomic RNA in patient samples. In order cope with potential fluctuations supply chain and testing demands enable expedient adaptation reagents assays on hand, we include details for three parallel methodologies (one- two-step singleplex one-step multiplex assays). diagnostic platforms described can be easily adapted by basic science research laboratories relatively short turnaround time. For complete execution...
ABSTRACT Centenarians provide a unique lens through which to study longevity, healthy aging, and resiliency. Moreover, models of human aging resilience disease that allow for the testing potential interventions are virtually non-existent. We obtained characterized over 50 centenarian offspring peripheral blood samples including those connected functional independence data highlighting resistance disability cognitive impairment. Targeted methylation arrays were used in molecular clocks...
The coronavirus disease 2019 (COVID-19) pandemic, caused by the severe acute respiratory syndrome 2 (SARS-CoV-2), is now a major global public health crisis. In United States, significant delays in rapid development and distribution of diagnostic testing for SARS-CoV-2 infection have prevented adequate COVID-19 patient care management impacting timely mapping dynamics viral spread general population, more topically, conservation personal protective equipment. Furthermore, vulnerable...
Abstract Special AT-rich sequence binding protein 2 (Satb2) is a matrix attachment region (MAR) protein. Satb2 impacts skeletal development by regulating gene transcription required for osteogenic differentiation. Although its role as high-order factor well supported, other roles in remain unclear. In particular, the impact of dosage sensitivity (heterozygous mutations) and variance on phenotypic severity still not understood. To further investigate molecular cellular mechanisms...
SUMMARY Hemogenic endothelial cells (HECs) are specialized that undergo to hematopoietic transition (EHT) give rise progenitors. Though not defined as a organ, the lung houses many resident cells, aids in platelet biogenesis, and is reservoir for stem progenitor (HSPCs), but HECs have never been described. Using explant cultures of murine human fetal lungs, we demonstrate source functional capacity EHT produce de-novo HSPCs. Flow cytometric assessment explants showed production HSPCs...
ABSTRACT Neuroendocrine tumors (NETs) are rare cancers that may arise in the gastrointestinal tract and pancreas. The fundamental mechanisms driving gastroenteropancreatic (GEP) NET growth remain incompletely elucidated; however, heterogeneous clinical behavior of GEP-NETs suggests both cellular lineage dynamics tumor microenvironment influence pathophysiology. Here, we investigated single-cell transcriptomes immune cells from patients with NETs. Malignant GEP-NET expressed genes regulons...
SUMMARY The human hematopoietic stem cell (HSC) harbors remarkable regenerative potential that can be harnessed therapeutically. During early development, HSCs in the fetal liver (FL) undergo active expansion while simultaneously retaining robust engraftment capacity, yet underlying molecular program responsible for their efficient remains unclear. We profiled 26,407 FL cells at both transcriptional and protein levels including over 7,000 highly enriched functional to establish a detailed...
Abstract Age-related disability and diseases are known to be delayed in people living 100 years or more. Changes the immune system with age known, including cell type composition gene expression differences. To further explore changes extreme longevity subjects, we investigated peripheral blood subpopulations across at a single resolution. We performed an integrative analysis of public scRNA-seq datasets define consensus types age, classified our novel New England Centenarian Study dataset....