- biodegradable polymer synthesis and properties
- Microplastics and Plastic Pollution
- Wastewater Treatment and Nitrogen Removal
- Enzyme Catalysis and Immobilization
- Microbial Applications in Construction Materials
- Carbon dioxide utilization in catalysis
- Microbial Metabolic Engineering and Bioproduction
- Recycling and Waste Management Techniques
- Microbial Fuel Cells and Bioremediation
- Building materials and conservation
- Pesticide and Herbicide Environmental Studies
- Graphene and Nanomaterials Applications
- Electrospun Nanofibers in Biomedical Applications
- Amino Acid Enzymes and Metabolism
- Concrete and Cement Materials Research
- Cassava research and cyanide
- Pharmaceutical and Antibiotic Environmental Impacts
- Recycled Aggregate Concrete Performance
- Constructed Wetlands for Wastewater Treatment
- Additive Manufacturing and 3D Printing Technologies
- Innovations in Concrete and Construction Materials
- Pesticide Exposure and Toxicity
- Microbial Community Ecology and Physiology
- Odor and Emission Control Technologies
- Coffee research and impacts
Universidade Nova de Lisboa
2010-2022
Rede de Química e Tecnologia
2010-2022
University of Lisbon
2015-2022
Laboratório Associado para a Química Verde
2021-2022
Fundação para a Ciência e Tecnologia
2010
Instituto de Tecnología Química
2003
Faculdade de Tecnologia e Ciências
1999
Abstract Activated sludge submitted to aerobic dynamic feeding conditions showed a good and stable capacity store polyhydroxybutyrate (PHB). The system, working for 2 years, selected microbial population with high PHB storage capacity. influence of carbon nitrogen concentrations on the accumulation yield was studied in range 15–180 Cmmol/l acetate 0–2.8 Nmmol/l ammonia. Low ammonia favored accumulation. maximum content, 67.5%, obtained 180 supplied one pulse. However, such substrate...
A culture of sulfate reducing bacteria (SRB) growing on lactate and was incubated at different pH values in the range 5.8-7.0. The effect growth rate determined this range; highest observed 6.7. Hydrogen sulfide produced from reduction found to have a direct reversible toxicity SRB. hydrogen Concentration 547 mg/L (16.1 mM) completely inhibited growth. Comparison between acetic acid inhibition is presented concomitant kinetics are mathematically described.
Mixed cultures submitted to acetate "feast" and "famine" cycles are able store intracellularly high quantities of polyhydroxybutyrate (PHB). It was demonstrated in a previous study that the intracellular PHB content can be increased up 78.5% (g HB/gVSS) cell dry weight sequencing batch reactor (SBR) with optimised operating conditions. The specific formation rate also shown higher for mixed than pure cultures. Such contents productivity open new perspectives industrial production...
The characterization of polyhydroxyalkanoates (PHA) produced by mixed cultures is fundamental for foreseeing the possible final applications polymer. In this study PHA under aerobic dynamic feeding (ADF) conditions are characterized. shows a stable average molecular weight ([symbol: see text]) in range (1.0-3.0) x 10(6), along three years reactor operation. Attempts to improve amount did not introduce significant variations on values [symbol: text]. Along period, polydispersity indices (PDI)...
Abstract Background This paper presents a metabolic model describing the production of polyhydroxyalkanoate (PHA) copolymers in mixed microbial cultures, using mixtures acetic and propionic acid as carbon source material. Material energetic balances were established on basis previously elucidated pathways. Equations derived for theoretical yields cell growth PHA functions oxidative phosphorylation efficiency, P/O ratio. The efficiency was estimated from rate measurements, which turn allowed...
A laboratory scale sequencing batch reactor (SBR) operating for enhanced biological phosphorus removal (EBPR) and fed with a mixture of volatile fatty acids (VFAs) showed stable efficient EBPR capacity over four-year-period. Phosphorus (P), poly-beta-hydroxyalkanoate (PHA) glycogen cycling consistent classical anaerobic/aerobic were demonstrated the order anaerobic VFA uptake being propionate, acetate then butyrate. The SBR was operated without pH control 63.67 +/- 13.86 mg P l-1 released...
An open mixed culture was enriched with glycogen-accumulating organisms (GAOs) by using a sequencing batch reactor and treating an agroindustrial waste (sugar cane molasses) under cyclic anaerobic-aerobic conditions. Over 1-year operating period, the exhibited very stable GAO phenotype average polyhydroxyalkanoate (PHA) content of 17% total suspended solids. However, microbial community evolved over course operation to exhibiting unusual characteristics in producing PHAs comprised...
The microbiology of denitrifying enhanced biological phosphorus removal systems has been a subject much debate. question centred on the affinities different types Candidatus Accumulibacter PAOs, type I and II, towards electron acceptors such as oxygen, nitrate nitrite. This study used propionate anaerobic/anoxic/aerobic lab-scale sequencing batch reactor where microbial culture was successfully enriched in organisms (approx. 90%). able to take up using nitrate, nitrite oxygen acceptors,...