- Chemical and Physical Properties in Aqueous Solutions
- Algal biology and biofuel production
- Ionic liquids properties and applications
- Seaweed-derived Bioactive Compounds
- Aquatic Ecosystems and Phytoplankton Dynamics
- Microbial Metabolites in Food Biotechnology
- Electrochemical sensors and biosensors
- Enzyme Catalysis and Immobilization
- Analytical Chemistry and Chromatography
- Biocrusts and Microbial Ecology
- Photochemistry and Electron Transfer Studies
- Meat and Animal Product Quality
- Dye analysis and toxicity
- Food Chemistry and Fat Analysis
- Phytochemicals and Antioxidant Activities
- Edible Oils Quality and Analysis
- Electrochemical Analysis and Applications
- Marine and coastal plant biology
- Photoreceptor and optogenetics research
- Photosynthetic Processes and Mechanisms
- Plant Growth Enhancement Techniques
- Microplastics and Plastic Pollution
- Chromatography in Natural Products
- Photodynamic Therapy Research Studies
- Microbial Metabolism and Applications
University of Aveiro
2016-2022
Bioactive compounds extracted from natural renewable sources have attracted increased interest both industry and academia.
In this work, a process for the extraction and purification of carotenoids from fruit Bactris gasipaes was developed. Ethanolic aqueous solutions ionic liquids (ILs) surfactants were evaluated on these pigments. Thus, we developed an optimized sustainable downstream mediated by best solvent with further isolation recyclability IL used. The characterized not only in terms efficiency but also regarding its environmental impact. solvents as well high (maximum yield = 88.7 ± 0.9...
Deep eutectic solvents as efficient to extract and purify collagen from codfish skins.
There is a strong industrial interest in the development of greener and more sustainable processes based on use renewable resources, biorefinery marine such as macroalgae, stands major opportunity toward that end. In this work, Saccharina latissima (Linnaeus), brown macroalga, was used source pigments to develop an integrated platform able promote extraction separation chlorophyll fucoxanthin one single step. The process studied, its operational conditions were optimized with yields 4.93 ±...
Abstract Luminescent solar concentrators (LSCs) are photovoltaic (PV) complementary devices to overcome the mismatch between Si‐based PV cells, response and spectrum, allowing urban integration. Challenges for luminescent layer include use of abundant sustainable natural organic molecules. Here, LSCs composed a glass container based on bundles cylindrical hollow‐core plastic optical fibers filled with aqueous solutions R‐phycoerythrin (R‐PE), extracted from Gracilaria sp. algae presented....
Spirulina sp. is a cyanobacterium rich in essential amino acids and pigments such as chlorophyll a, xanthophylls, phycocyanin. Besides many other applications, its derivatives are being studied photosensitizers photodynamic therapy for cancer treatment. In this work, two methodologies of solid–liquid extraction were developed, their performance compared; one using conventional organic solvents the aqueous solutions ionic liquids (ILs) surfactants. It was found that an solution ammonium-based...
The sustainable cellular delivery of the pleiotropic drug curcumin encounters drawbacks related to its fast autoxidation at physiological pH, cytotoxicity vehicles and poor uptake. A biomaterial compatible with appropriate structure allow correct encapsulation considering solubility in water, while maintaining stability for a safe release was developed. In this work, developed started by preparation an oil-in-water nanoemulsion using cytocompatible copolymer (Pluronic F 127) coated...
Abstract The Internet of Things (IoT) fosters the development smart city systems for sustainable living and increases comfort people. One current challenges buildings is optimization energy management. Temperature monitoring in prime importance, as heating account a great part total consumption. Here, solar optical temperature sensor presented with thermal sensitivity up to 1.23% °C −1 based on aqueous solutions enhanced green fluorescent protein C‐phycocyanin from biological feedstocks....
Green fluorescent protein (GFP) has excellent properties as a biosensor and biomarker; however, its widespread use is limited by purification costs. Alternative low-cost techniques can overcome this issue. The aim of work was to evaluate aqueous biphasic systems (ABS) composed cholinium chloride ([Ch]Cl) different polymers effective platforms recover GFP from cell lysate recombinant Escherichia coli BL21. All completely extracted (>99%) into the polymeric- or [Ch]Cl-rich phases. In general,...
The ability of polymeric-based ABS to fractionate phenolic compounds from lignin depolymerisation was assessed.
Besides the good performance, downstream process based in thermo-responsive systems was shown to be efficient and of lower environmental impact.
Abstract BACKGROUND The demand for colorants from natural bio‐based sources is increasing. Violacein a purple‐blue hydrophobic pigment with interesting bioactivity whose expression in genetically modified Yarrowia lipolytica production was successfully achieved. RESULTS In this work, several surfactants were tested the extraction of violacein Y. cells, and operational conditions optimized to maximize yield. After optimization, purification using aqueous biphasic systems (ABS) composed Tween...
Green fluorescent protein was used to fabricate planar LSCs in liquid and solid state, yielding competitive power conversion efficiency values stating the potential of naturally-based molecules development sustainable LSCs.
Phycobiliproteins are fluorescent proteins mainly produced by red macroalgae and cyanobacteria. These proteins, essential to the survival of these organisms, find application in many fields interest, from medical, pharmaceutical, cosmetic food textile industries. The biggest obstacle their use is lack simple environmental economical sustainable methodologies obtain with high purity. In this work, a new purification process proposed based on induced precipitation target followed...
Abstract Twelve surface‐active ionic liquids (SAILs) and derivatives, based on imidazolium, ammonium, phosphonium cations containing one, or more, long alkyl chains in the cation and/or anion, were synthetized characterized. The aggregation behavior of these SAILs water, as well their adsorption at solution/air interface, studied by assessing surface tension conductivity. CMC values obtained (0.03–6.0 mM) show a high propensity compounds to self‐aggregate aqueous media. Their thermal...
The poor photochemical stability of R-phycoerythrin (R-PE) has been a bottleneck for its broad-spectrum applications. Inspired by nature, we studied sustainable strategy protein cohabitation to enhance R-PE embedding it in solid matrix gelatin. Both pure and fresh phycobiliprotein (PBP) extracts recovered from Gracilaria gracilis were studied. incorporation the gelatin-based films (gelatin-RPE gelatin-PBPs) improved at least 8 months, longest time period reported so far. These results...