A5030383547- Ionic liquids properties and applications
- Chemical and Physical Properties in Aqueous Solutions
- Morinda citrifolia extract uses
- Microbial Metabolism and Applications
- Electrochemical Analysis and Applications
- Electrochemical sensors and biosensors
- Crystallization and Solubility Studies
- Advanced Fluorescence Microscopy Techniques
- Surfactants and Colloidal Systems
- Analytical Chemistry and Chromatography
- Protein purification and stability
- Microbial Metabolites in Food Biotechnology
- Advanced Biosensing Techniques and Applications
- Mycotoxins in Agriculture and Food
- Molecular Sensors and Ion Detection
- Material Selection and Properties
- Water Quality and Pollution Assessment
- Monoclonal and Polyclonal Antibodies Research
- Pigment Synthesis and Properties
- Public Health in Brazil
- Process Optimization and Integration
- Chromatography in Natural Products
- Plant and fungal interactions
- Electrolyte and hormonal disorders
- Apelin-related biomedical research
Universidade de Ribeirão Preto
2025
Universidade de São Paulo
2022-2024
Universidade Estadual Paulista (Unesp)
2014-2024
Instituto de Ciências Farmacêuticas
2014-2023
Universidade Cidade de São Paulo
2021-2023
University of Miami
2022-2023
RMIT University
2020-2021
Centro Universitário de Araraquara
2019
Universidade Federal de Minas Gerais
2015
Biorefinery is the ideal model to help marine industries apply green and blue economy principles towards a more sustainable, profitable, conscious ocean economy.
Abstract Biopharmaceuticals are one of the most important groups biotechnological products, representing one‐quarter all pharmaceutical sales and providing suitable efficient medical care for many previously untreatable diseases. However, their production purification usually require complex processes, resulting in extremely expensive final products. Thus, development newer, simpler cheaper methods biosynthesis these biocompounds, as well proper integration between stages, crucial to reduce...
Green fluorescent protein (GFP) has excellent properties as a biosensor and biomarker; however, its widespread use is limited by purification costs. Alternative low-cost techniques can overcome this issue. The aim of work was to evaluate aqueous biphasic systems (ABS) composed cholinium chloride ([Ch]Cl) different polymers effective platforms recover GFP from cell lysate recombinant Escherichia coli BL21. All completely extracted (>99%) into the polymeric- or [Ch]Cl-rich phases. In general,...
Fluorescent proteins have many applications as biomarkers and biosensors in the medical biological fields. Their success was largely supported by modifications of first isolated fluorescent protein, wild-type Green Protein (wtGFP), which allowed development improved variants such Enhanced GFP (EGFP). The reports on EGFP indicated that protein presented a single form fluorescence peak, contrast to two conformations observed wtGFP. However, after experimental determination crystalline...
Fluorescent Proteins (FP) can be applied as biomarkers and biosensors in the industrial medical fields, but their large-scale use, especially for new applications, is limited due to low stability. Hence, discovery of additives capable preserving activity FP at room temperature under stress conditions help expand facilitate commercial use. With this goal, we evaluated application 1-alkyl-3-methylimidazolium chloride-based ([Cnmim]Cl) ionic liquids (ILs) preserve Enhanced Green Protein (EGFP)...
Solvents that stabilize protein structures can improve and expand their biochemical applications, particularly with the growing interest in biocatalytic-based processes. Aiming to select novel solvents for stabilization, we explored effect of alkylammonium nitrate protic ionic liquids (PILs)-water mixtures increasing cation alkyl chain length on lysozyme conformational stability. Four PILs were studied, is, ethylammonium (EAN), butylammonium (BAN), hexylammonium (HAN), octylammonium (OAN)....
Green fluorescent protein (GFP) and its variants are widely used in medical biological research, especially acting as indicators of structural integrity, protein-protein interactions biosensors. This study employs superfolder GFP (sfGFP) to investigate the impact varying alkyl-chain length 1-Cn-3-methylimidazolium chloride ionic liquid (IL) series ([Cnmim]Cl, n = 2, 4, 6, 8, 10, 12) on fluorescence, structure, hydration, aggregation dynamics crystallization behaviour. The results revealed a...
Green fluorescent protein (GFP) is a globular used as biosensor and biomarker in medical industrial fields. However, due to the expensive production costs of expressing proteins using high-cost inducers like isopropyl-β-d-1-thiogalactopyranoside (IPTG), number GFP applications are still scarce. This work studied enhanced (EGFP) Escherichia coli BL21 (DE3) [pLysS; pET28(a)], aiming increase its yield reduce costs. First, influence agitation rate, induction time, concentration IPTG EGFP was...
Brucella species can cause brucellosis, a zoonotic disease that causes serious livestock economic losses and represents public health threat. The mechanism of virulence spp. is not yet fully understood. Therefore, it crucial to identify new molecules serve as factors better understand this host-pathogen interplay. Here, we evaluated the role membrane fusogenic protein (Mfp) outer 19 (Omp19) in bacterial pathogenesis. In study, showed B. abortus Δmfp::kan Δomp19::kan deletion mutant strains...
Aqueous biphasic systems (ABSs) based on sodium polyacrylate (NaPA), ethylene oxide/propylene oxide (EO/PO) polymers, and (EO)x-(PO)y-(EO)x triblock copolymers were prepared applied aiming at continuous fructooligosaccharide (FOS) production separation. EO/PO hydrophilicity/hydrophobicity balance had a significant effect ABS formation. To develop an integrated process including the enzymatic (levansucrase) of FOSs their purification while improving yield by further glucose separation,...
This study aims to demonstrate the potential of lactic acid bacteria (LAB) Pediococcus pentosaceus LBM18 against mycotoxin-producing Alternaria alternata TEF-1A and highlight its application as an effective grain silage inoculant control mycotoxin contamination.The antifungal properties Ped. lyophilized (PPL) were assessed by evaluating effect on A. grown in a corn silage-based medium, which included morphological changes Scanning Electron Microscopy (SEM) observations, growth rate, conidia...