A5021144926- Brucella: diagnosis, epidemiology, treatment
- Inflammasome and immune disorders
- Trypanosoma species research and implications
- Galectins and Cancer Biology
- interferon and immune responses
- Immunotherapy and Immune Responses
- Research on Leishmaniasis Studies
- Thyroid Disorders and Treatments
- Tuberculosis Research and Epidemiology
- Parasitic Infections and Diagnostics
- vaccines and immunoinformatics approaches
- Atherosclerosis and Cardiovascular Diseases
- Mycobacterium research and diagnosis
- Escherichia coli research studies
- Biochemical and Molecular Research
- Gout, Hyperuricemia, Uric Acid
- Folate and B Vitamins Research
- Pediatric Urology and Nephrology Studies
- Global Security and Public Health
- Burkholderia infections and melioidosis
- Fatty Acid Research and Health
- Autophagy in Disease and Therapy
- Infectious Diseases and Tuberculosis
- Immune Response and Inflammation
- Streptococcal Infections and Treatments
The University of Texas Southwestern Medical Center
2022-2024
Southwestern Medical Center
2024
Universidade Federal de Minas Gerais
2012-2021
Pathogens are detected by innate immune receptors that, upon activation, orchestrate an appropriate response. Recent studies revealed the intracellular signaling cascades involved in TLR-initiated response to Brucella abortus infection. However, no report has elucidated role of inflammasome recognition. Therefore, we decided investigate function NLRC4, NLRP3, and AIM2 sensing Brucella. In this study, showed that NLRC4 is not required induce caspase-1 activation further secretion IL-1β B....
Brucella abortus is a facultative intracellular bacterium that causes brucellosis, prevalent zoonosis leads to abortion and infertility in cattle, undulant fever, debilitating arthritis, endocarditis, meningitis humans. Signaling pathways triggered by B. involves stimulator of IFN genes (STING), which production type I IFNs. In this study, we evaluated the pathway linking unfolded protein response (UPR) endoplasmic reticulum-resident transmembrane molecule STING, during infection. We...
Brucella abortus is a Gram-negative intracellular bacterial pathogen that causes zoonosis of worldwide occurrence, leading to undulant fever in humans and abortion domestic animals. B. recognized by several pattern-recognition receptors triggering pathways during the host innate immune response. Therefore, here, we determined cooperative role TLR9 with TLR2 or TLR6 sensing Furthermore, deciphered response against its DNA, emphasizing TLR9-MAPK/NF-κB signaling production proinflammatory...
Brucella abortus is the causative agent of brucellosis, which causes abortion in domestic animals and undulant fever humans. This bacterium infects proliferates mainly macrophages dendritic cells, where it recognized by pattern recognition receptors (PRRs) including Nod‐like (NLRs). Our group recently demonstrated role AIM2 NLRP3 recognition. Here, we investigated participation NLRP12 innate immune response to B. . We show that inhibits early production IL‐12 bone marrow‐derived upon...
<i>Mycobacterium tuberculosis</i> (Mtb) infection remains a major public health concern. The STING (stimulator of interferon genes) pathway contributes to the cytosolic surveillance host cells. Most studies on role activation in Mtb have focused macrophages. Moreover, detailed investigation during vivo is required. Here, we deciphered involvement dendritic cells (DCs) and response vivo. In DCs, this adaptor molecule was important for <i>Ifn-β</i> expression IL-12...
Components of the DNA mismatch repair (MMR) pathway are major players in processes known to generate genetic diversity, such as mutagenesis and recombination. Trypanosoma cruzi, protozoan parasite that causes Chagas disease has a highly heterogeneous population, composed pool strains with distinct characteristics. Studies number molecular markers identified up six groups T. cruzi which showed levels variability. To investigate basis for differences, we analyzed MSH2 gene, encodes key...
The oxidative lesion 8-oxoguanine (8-oxoG) is removed during base excision repair by the DNA glycosylase 1 (Ogg1). This can erroneously pair with adenine, and of this damaged Ogg1 enables insertion a guanine prevents mutation. In report, we identified characterized from protozoan parasite Trypanosoma cruzi (TcOgg1), causative agent Chagas disease. Like most living organisms, T. susceptible to stress, hence essential for its survival improvement infection. We verified that TcOGG1 gene encodes...
Brucella abortus is a Gram-negative, facultative intracellular bacterium that causes brucellosis, worldwide zoonotic disease leading to undulant fever in humans and abortion cattle. The immune response against this relies on the recognition of microbial pathogen-associated molecular patterns, such as lipoproteins, lipopolysaccharides, DNA; however, immunostimulatory potential B. RNA remains be elucidated. Here, we show dendritic cells (DCs) produce significant amounts IL-12, IL-6,...
Specific DNA repair pathways from Trypanosoma cruzi are believed to protect genomic and kinetoplast (kDNA) mutations. Particular supposed operate in order nucleotides oxidized by reactive oxygen species (ROS) during parasite infection, being 7,8-dihydro-8-oxoguanine (8oxoG) a frequent highly mutagenic base alteration. If unrepaired, 8oxoG can lead cytotoxic transversions replication. In mammals, polymerase beta (Polβ) is mainly involved excision (BER) of oxidative damage. However its...
NLRP3 inflammasome is a protein complex crucial to caspase-1 activation and IL-1β IL-18 maturation. This receptor participates in innate immune responses different pathogens, including the bacteria of genus Brucella. Our group recently demonstrated that Brucella abortus-induced secretion involves it partially dependent on mitochondrial ROS production. However, other factors could be involved, such as P2X7-dependent potassium efflux, membrane destabilization, cathepsin release. Moreover,...
Background DNA repair mechanisms are crucial for maintenance of the genome in all organisms, including parasites where successful infection is dependent both on genomic stability and sequence variation. MSH2 an early acting, central component Mismatch Repair (MMR) pathway, which responsible recognition correction base mismatches that occur during replication recombination. In addition, recent evidence suggests might also play important, but poorly understood, role responding to oxidative...
Brucella spp. infection is frequently acquired through contaminated aerosols. The role of interleukin-1 beta (IL-1β) in the early pulmonary response to respiratory unknown. As shown here, IL-1β levels lung homogenates and bronchoalveolar lavage fluid (BALF) mice intratracheally inoculated with B. abortus were increased at 3 7 days p.i. At p.i., CFU numbers higher IL-1 receptor (IL-1R) knockout (KO) than wild type (WT) mice. different times lungs BALF lacking some inflammasome components...
The immunoproteasome is a specific proteasome isoform composed of three subunits, termed β1i, β2i, and β5i. Its proteolytic activity enhances the quantity quality peptides to be presented by major histocompatibility complex class I (MHC-I) molecules CD8+ T cells. However, role combined deficiency subunits in protective immunity against bacterial pathogens has not been investigated. In this study, we addressed during infection Brucella abortus, an intracellular bacterium that requires cell...
Brucella species can cause brucellosis, a zoonotic disease that causes serious livestock economic losses and represents public health threat. The mechanism of virulence spp. is not yet fully understood. Therefore, it crucial to identify new molecules serve as factors better understand this host-pathogen interplay. Here, we evaluated the role membrane fusogenic protein (Mfp) outer 19 (Omp19) in bacterial pathogenesis. In study, showed B. abortus Δmfp::kan Δomp19::kan deletion mutant strains...
Brucella abortus is a Gram-negative bacterium responsible for worldwide zoonotic infection—Brucellosis, which has been associated with high morbidity rate in humans and severe economic losses infected livestock. The natural route of infection through oral nasal mucosa but the invasion process host gut yet to be understood. Studies have examined role NLRP6 (NOD-like receptor family pyrin domain-containing-6 protein) homeostasis defense against pathogens. Here, we investigated impact...
In this study, we provide evidence that galectin-3 (Gal-3) plays an important role in Brucella abortus infection. Our results showed increased Gal-3 expression and secretion B. infected macrophages mice. Additionally, our findings indicate is dispensable for Brucella-containing vacuoles disruption, inflammasome activation pyroptosis. On the other hand, observed Brucella-induced crucial induction of molecules associated to type I IFN signalling pathway, such as IFN-β: Interferon beta (IFN-β),...
A total of 880 expressed sequence tags (EST) originated from clones randomly selected a Trypanosoma cruzi amastigote cDNA library have been analyzed. Of these, 40% (355 ESTs) identified by similarity to sequences in public databases and classified according functional categorization their putative products. About 11% the mRNAs amastigotes are related translational machinery, large number them (9% library) encode ribosomal proteins. comparative analysis with previous study, where same were...
The ST2 receptor plays an important role in the gut such as permeability regulation, epithelium regeneration, and promoting intestinal immune modulation. Here, we studied of a murine model oral infection with Brucella abortus, its influence on homeostasis control bacterial replication. Balb/c (wild-type, WT) deficient mice (ST2−/−) were infected by gavage results obtained at 3 14 days post (dpi). Our suggest that ST2−/− are more resistant to B. abortus infection, lower colony-forming unit...
Abstract Lung disease due to non-tuberculous mycobacteria (NTM) is rising in incidence. While both two dimensional cell culture and animal models exist for NTM infections, a major knowledge gap the early responses of human alveolar innate immune cells within microenvironment. Here we describe development humanized, three-dimensional, alveolus lung-on-a-chip (ALoC) model Mycobacterium fortuitum lung infection that incorporates only primary such as pulmonary vascular endothelial channel, type...