A5040975103- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Helminth infection and control
- Synthesis and Biological Evaluation
- Mitochondrial Function and Pathology
- Traditional and Medicinal Uses of Annonaceae
- Insect and Pesticide Research
- Parasites and Host Interactions
- Insect Pest Control Strategies
- Insect symbiosis and bacterial influences
- Cardiomyopathy and Myosin Studies
- Phytochemical compounds biological activities
- Lysosomal Storage Disorders Research
- Calcium signaling and nucleotide metabolism
- Ion channel regulation and function
- Malaria Research and Control
- Vector-borne infectious diseases
- Nitric Oxide and Endothelin Effects
- Essential Oils and Antimicrobial Activity
- Piperaceae Chemical and Biological Studies
- Adipose Tissue and Metabolism
- Bipolar Disorder and Treatment
- Biochemical and Molecular Research
- Pesticide Exposure and Toxicity
- Click Chemistry and Applications
Universidade Estadual de Campinas (UNICAMP)
2015-2024
Fundação Oswaldo Cruz
2020
Universidad Católica Cecilio Acosta
2020
University of Wisconsin–Platteville
2020
Hospital de Clínicas da Unicamp
2011-2012
University of Illinois Urbana-Champaign
1990-1993
Urbana University
1991
Universidade Federal do Rio de Janeiro
1988-1989
Digitonin can be used to permeabilize selectively the plasma membrane of Trypanosoma cruzi epimastigotes without significantly affecting functional integrity mitochondria. Addition digitonin at concentrations close 64 microM caused decrease in rate basal respiration similar that by oligomycin. A further addition carbonyl cyanide p-trifluorophenylhydrazone (FCCP) brought same observed prior inclusion or This suggests like oligomycin, is shifting a nonphosphorylating state probably depleting...
The main consequence of oxidative stress is the formation DNA lesions, which can result in genomic instability and lead to cell death. Guanine base that most susceptible oxidation, due its low redox potential, 8-oxoguanine (8-oxoG) common lesion. These characteristics make 8-oxoG a good cellular biomarker indicate extent stress. If not repaired, pair with adenine cause G:C T:A transversion. When inserted during replication, it could generate double-strand breaks, makes this lesion...
The epimastigote stage of Trypanosoma cruzi undergoes PCD (programmed cell death) when exposed to FHS (fresh human serum). Although it has been known for over 30 years that complement is responsible FHS-induced death, the link between activation and triggering not established. We have previously shown mitochondrion participates in orchestration this model. Several changes mitochondrial function were described, particular was mitochondrion-derived O(2)(*-) (superoxide radical) necessary PCD....
The isolation and identification of a series new pseudoceratidine (1) derivatives from the sponge Tedania brasiliensis enabled evaluation their antiparasitic activity against Plasmodium falciparum, Leishmania (Leishmania) amazonensis, infantum, Trypanosoma cruzi, causative agents malaria, cutaneous leishmaniasis, visceral Chagas disease, respectively. 3-debromopseudoceratidine (4), 20-debromopseudoceratidine (5), 4-bromopseudoceratidine (6), 19-bromopseudoceratidine (7),...
Chagas disease, caused by the protozoan parasite Trypanosoma cruzi, presents a variable clinical course, varying from asymptomatic to serious debilitating pathologies with cardiac, digestive or cardio-digestive impairment. Previous studies using two clonal T. cruzi populations, Col1.7G2 (T. I) and JG II) demonstrated that there was differential tissue distribution of these parasites during infection in BALB/c mice, predominance heart. To date little is known about mechanisms determine this...
High-affinity Ca(2+)-activated ATPases that do not show any demonstrable dependence on Mg2+ have been reported in the plasma membranes of different trypanosomatids, and it has suggested [McLaughlin (1985) Mol. Biochem. Parasitol. 15, 189-201; Ghosh, Ray, Sarkar & Bhaduri (1990) J. Biol. Chem. 265, 11345-11351] these enzymes may a role Ca2+ transport by membrane regulation intracellular parasites. In this report we investigated Trypanosoma cruzi vesicles using Arsenazo III as indicator....
The essential oils from the leaves of Annona pickelii and salzmannii (Annonaceae) were obtained by hydrodistillation using a Clevenger apparatus, analysed GC-MS GC-FID. A total 21 compounds identified in oil A. 23 that salzmannii; sesquiterpenes predominated both oils. Bicyclogermacrene (38.0%), (E)-caryophyllene (27.8%), α-copaene (6.9%) α-humulene (4.0%) main components pickelii, while δ-cadinene (22.6%), (21.4%), (13.3%), bicyclogermacrene (11.3%) germacrene D salzmannii. biological...
Hypoxia (low oxygen tension) is a common feature of inflamed and infected tissues. The influence hypoxia on macrophage responses to micro-organisms has only recently been studied. This study demonstrates that induced macrophages control Leishmania amazonensis, an intracellular parasite causes cutaneous metastatic lesions. mechanisms contribute the against L. amazonensis infection under hypoxic microenvironment are not known. Nitric oxide, TNF-α, IL-10 or IL-12 responsible for decrease in...
Phospholipase A2 toxins present in snake venoms interact with biological membranes and serve as structural models for the design of small peptides anticancer, antibacterial antiparasitic properties. Oligoarginine are capable increasing cell membrane permeability (cell penetrating peptides), this reason interesting delivery systems compounds pharmacological interest. Inspired by these two families bioactive molecules, we have synthesized 13-mer potential antileishmanial leads gaining insights...
Novel gold( i )(NHC) containing thiopyrimidines and thiazolidines with promising antiviral antiparasitic activity.
Components of the DNA mismatch repair (MMR) pathway are major players in processes known to generate genetic diversity, such as mutagenesis and recombination. Trypanosoma cruzi, protozoan parasite that causes Chagas disease has a highly heterogeneous population, composed pool strains with distinct characteristics. Studies number molecular markers identified up six groups T. cruzi which showed levels variability. To investigate basis for differences, we analyzed MSH2 gene, encodes key...
The essential oil from the leaves of Annona vepretorun was obtained by hydrodistillation using a Clevenger-type apparatus and analyzed GC-MS GC-FID. Eighteen compounds representing 98.1% crude were identified. major identified bicyclogermacrene (43.7%), spathulenol (11.4%), alpha-felandrene (10.0%), alpha-pinene (7.1%), (E)-beta-ocimene (6.8%), germacrene D (5.8%), p-cymene (4.2%). trypanocidal activity against Trypanosoma cruzi epimastigote forms, as well as, antimicrobial antioxidant...
Phytochemical investigation of the hexane extract from stem Xylopia laevigata led to isolation ent-kaurane diterpenoids, ent-kaur-16-en-19-oic acid, 4-epi-kaurenic ent-16β-hydroxy-17-acetoxy-kauran-19-al, ent-3β-hydroxy-kaur-16-en-19-oic and ent-16β,17-dihydroxy-kauran-19-oic as well spathulenol a mixture β-sitosterol, stigmasterol campesterol. The identification compounds was performed on basis spectrometric methods including GC-MS, IR, 1D 2D NMR. Potent larvicidal activity against Aedes...