A5001863109- Brucella: diagnosis, epidemiology, treatment
- Galectins and Cancer Biology
- Escherichia coli research studies
- Burkholderia infections and melioidosis
- Immunotherapy and Immune Responses
- Moringa oleifera research and applications
- Salmonella and Campylobacter epidemiology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Response and Inflammation
- Phytochemistry and Biological Activities
- Toxin Mechanisms and Immunotoxins
- Traditional and Medicinal Uses of Annonaceae
- Phytochemistry and biological activities of Ficus species
- Natural Compounds in Disease Treatment
- Monoclonal and Polyclonal Antibodies Research
- Sepsis Diagnosis and Treatment
- Intensive Care Unit Cognitive Disorders
- Phytochemical compounds biological activities
- Ginger and Zingiberaceae research
- Animal Diversity and Health Studies
- Natural Antidiabetic Agents Studies
- interferon and immune responses
- Atherosclerosis and Cardiovascular Diseases
- Inflammasome and immune disorders
- Nitric Oxide and Endothelin Effects
University of Buenos Aires
2013-2025
Consejo Nacional de Investigaciones Científicas y Técnicas
2017-2025
National University of Rosario
2007-2021
Institute of Astronomy and Space Physics
2013-2018
Instituto de Química y Fisicoquímica Biológicas
2011
Tissue injury leads to the release of uric acid (UA). At high local concentrations, UA can form monosodium urate crystals (MSU). MSU and stimulate neutrophils extracellular traps (NET). Here, we investigated whether these NET could be involved in development inflammation by stimulating cytokine airway epithelial cells. We found that significantly increased secretion CXCL8/IL-8 IL-6 alveolar bronchial These effects were not observed when NETosis was inhibited Diphenyleneiodonium, elastase...
Introduction Swine brucellosis, caused by Brucella suis , is a worldwide infectious zoonotic disease. Currently, there are no available human or porcine vaccines to protect against B. infection, which primarily acquired through the mucosa. We recently described MapB, homologous protein of TamB, inner membrane component TAM system. Our findings indicate that MapB involved in bacterial cell envelope homeostasis. In this study, we characterize outer vesicles (OMVs) 1330 (wt) and those Δ mapB (Δ...
ABSTRACT Alveolar macrophages (AM) seem to constitute the main cellular target of inhaled brucellae. Here, we show that Brucella abortus invades and replicates in murine AM without inducing cytotoxicity. B. infection induced a statistically significant increase tumor necrosis factor alpha (TNF-α), CXCL1 or keratinocyte chemoattractant (KC), interleukin-1β (IL-1β), IL-6, IL-12 from C57BL/6 mice BALB/c mice, but these responses were generally weaker and/or delayed compared those elicited...
Trophoblasts are targets of infection by Brucella spp. but their role in the pathophysiology pregnancy complications brucellosis is unknown. Here we show that abortus invades and replicates human trophoblastic cell line Swan-71 intracellular survival bacterium depends on a functional virB operon. The elicited significant increments interleukin 8 (IL8), monocyte chemotactic protein 1 (MCP-1), IL6 secretion, levels IL1beta tumor necrosis factor-alpha (TNF-alpha) did not vary significantly....
Both CCL20 and human β-defensin 2 (hBD2) interact with the same membrane receptor display chemotactic antimicrobial activities. They are produced by airway epithelia in response to infectious agents proinflammatory cytokines. Whereas Brucella spp. can infect humans through inhalation, their ability induce hBD2 lung cells is unknown. Here we show that B. abortus induces expression alveolar (A549) or bronchial (Calu-6) epithelial cell lines, primary cells, monocytes, monocyte-derived...
Brucella enters their hosts mostly through mucosae from where it spreads systemically. Adhesion to extracellular matrix (ECM) components or host cells is important for the infectious process, and mediated by several adhesins, including BtaF trimeric autotransporter. Although Th1 responses gamma interferon (IFN-γ) are protection, antibodies able block adhesions might also contribute prevent infection. We evaluated importance of respiratory infection, characterized immune response protection...
Despite the importance of respiratory route for Brucella transmission, lung immune response to this pathogen is scarcely characterized. We investigated role cGAS/STING pathway microbial DNA recognition in control infection. After vitro B. abortus infection, CFU numbers were significantly higher alveolar macrophages (AM) and explants from STING KO mice than samples wild type (WT) mice, but no difference was observed cGAS samples. also increased WT AM epithelial cells preincubated with...
Brucella spp. infection is frequently acquired through contaminated aerosols. The role of interleukin-1 beta (IL-1β) in the early pulmonary response to respiratory unknown. As shown here, IL-1β levels lung homogenates and bronchoalveolar lavage fluid (BALF) mice intratracheally inoculated with B. abortus were increased at 3 7 days p.i. At p.i., CFU numbers higher IL-1 receptor (IL-1R) knockout (KO) than wild type (WT) mice. different times lungs BALF lacking some inflammasome components...
Brucella canis infection is an important cause of late-term abortion in pregnant bitches. The pathophysiological mechanisms leading to B. canis–induced are unknown, but heavily infected trophoblasts consistently observed. As responses other pathogens contribute placental inflammation abortion, the aim present study was characterize cytokine response canine infection. To achieve this, isolated from term placenta healthy female dogs were with canis, culture supernatants harvested for...
In spite of the frequent acquisition Brucella infection by oral route in humans, interaction bacterium with cells intestinal mucosa has been poorly studied. Here, we show that different species can invade human colonic epithelial cell lines (Caco-2 and HT-29), which only smooth replicate efficiently. Infection strains did not produce a significant cytotoxicity, while rough strain RB51 was more cytotoxic. Caco-2 or HT-29 either result an increased secretion TNF-α, IL-1β, MCP-1, IL-10 TGF-β as...
Although inhalation of infected aerosols is a frequent route for Brucella infection in humans, it rarely causes pulmonary clinical manifestations, suggesting mild or nearly absent local inflammatory response. The goal this study was to characterize the early innate immune response intratracheal with abortus mice and evaluate whether modulated by pathogen. After 106 CFU B. abortus, bacterial burden at 7 days post-infection (p.i.) comparable initial inoculum, despite an transient decline....
The study of capture and processing antigens (Ags) by intestinal epithelial cells is very important for development new oral administration systems. Efficient Ag delivery systems must resist enzymatic degradation gastric proteases deliver the across biological barriers. recombinant unlipidated outer membrane protein from Brucella spp. (U-Omp19) a protease inhibitor with immunostimulatory properties used as adjuvant in vaccine formulations. In present work we further characterized its...
Infection by Brucella species in pregnant animals and humans is associated with an increased risk of abortion, preterm birth, transmission the infection to offspring. The pathogen has a marked tropism for placenta uterus ability invade replicate within cells maternal-fetal unit, including trophoblasts decidual cells. Placentitis common finding infected animals. Several proinflammatory factors have been found be both Brucella-infected or vitro. As normal pregnancies require anti-inflammatory...
Adhesion to host cells is a key step for successful infection of many bacterial pathogens and may define tropism different tissues. To do so, bacteria display adhesins on their surfaces. Brucella an intracellular pathogen capable proliferating in wide variety cell types. It has been described that BmaC, large protein belongs the classical (type Va) autotransporter family, required efficient adhesion suis strain 1330 epithelial fibronectin. Here we show B. harbors two other type Va...
We tested the in vivo and vitro effects of both Ligaria cuneifolia catechin- quercetin-enriched fractions on erythrocyte shape deformability, plasma cholesterol level. For studies, adult male Wistar rats were randomized
It was demonstrated that Ligaria cuneifolia (Lc) crude extract increased blood viscosity and decreased plasma cholesterol in rats. In the present study, we analyzed Lc proanthocyanidin enriched fraction (PLc) to determine if it is capable of altering hemorheological parameters while diminis hing cholesterol. vivo studies adult male Wistar rats, randomized three groups (n = 6 each one) were performed: 1. Control: saline intraperitoneal (i.p.); 2. PLc 0.6 mg/100 g body weight (b.w.) i.p. 3. 3...
Brucella spp. have been associated with abortion in humans and animals. Although the mechanisms involved are not well established, it is known that placental infection accompanied by inflammatory phenomena. The ability of abortus to infect survive human endometrial stromal cells (T-HESC cell line) cytokine response elicited were evaluated. B. was able proliferate both non-decidualized decidualized T-HESC cells. Intracellular proliferation depended on expression a functional virB operon...