A5080107532- Viral-associated cancers and disorders
- Lymphoma Diagnosis and Treatment
- Histiocytic Disorders and Treatments
- Eosinophilic Disorders and Syndromes
- Parvovirus B19 Infection Studies
- Plant Virus Research Studies
- T-cell and Retrovirus Studies
- Intensive Care Unit Cognitive Disorders
- Cell death mechanisms and regulation
- Sepsis Diagnosis and Treatment
- Cytomegalovirus and herpesvirus research
- Biomedical Ethics and Regulation
- Chromatin Remodeling and Cancer
- Neuroblastoma Research and Treatments
- interferon and immune responses
- NF-κB Signaling Pathways
- Cannabis and Cannabinoid Research
- Neuroscience and Neuropharmacology Research
- Genetic factors in colorectal cancer
- Respiratory Support and Mechanisms
- Plant Disease Resistance and Genetics
- Glioma Diagnosis and Treatment
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Nerve injury and regeneration
- Toxin Mechanisms and Immunotoxins
Consejo Nacional de Investigaciones Científicas y Técnicas
2017-2025
Instituto Multidisciplinario de Biología Celular
2021-2025
Hospital General de Niños Ricardo Gutierrez
2010-2022
Fondazione IRCCS Istituto Neurologico Carlo Besta
2007
One hundred thirty-eight new Epstein-Barr virus (EBV) genome sequences have been determined. twenty-five of these and 116 from previous reports were combined to produce a multiple-sequence alignment 241 EBV genomes, which we used analyze variation within the viral genome. The type 1/type 2 classification remains major form is defined mostly by EBNA2 EBNA3, but single-nucleotide polymorphisms (SNPs) at EBNA3 locus extend into adjacent gp350 gp42 genes, whose products mediate infection B cells...
Most genomic studies on Epstein–Barr virus variability have focused the geographic and pathological associations of EBV1 genomes. In contrast, EBV2 genomes has been less explored, mainly due to their restricted circulation lesser number sequenced isolates. this study, we analyzed twenty-eight ten a potential recombinant from Argentina, which were combined with two-hundred-and-thirty-nine downloaded complete other regions, produce an initial multi-sample.vcf file comprising 278 EBV context,...
Introduction Epstein-Barr virus (EBV)-associated tumors show different expression patterns of latency genes. Since in breast carcinoma this pattern is not yet fully described, our aim was to characterize EBV positive series. Methods The study conducted on 71 biopsies and 48 non-neoplastic controls. EBNA1, LMP2A LMP1 assessed by immunohistochemistry with monoclonal antibodies, while viral genomic DNA EBERs RNA transcripts performed situ hybridization. presence confirmed PCR. Results EBNA1...
Background The etiology and the molecular mechanisms related to breast carcinogenesis remain poorly understood. Some recent reports have examined role of Human Papillomavirus (HPV) in this disease. purpose study was determine prevalence HPV cancer. Methods Sixty one fresh frozen cancers samples were analyzed. Samples tested for by PCR, products automatically sequenced. Findings correlated with clinical pathological characteristics. Results DNA cancer 26% (16/61). Clinical parameters not...
Epstein Barr virus (EBV) infection in Argentina occurs at an early age and occasionally develops infectious mononucleosis (IM). EBV is also related with lymphomas. LMP1, the viral oncoprotein polymorphic used to define variants. Aim To study LMP1 variants distribution among children EBV+ malignant benign conditions as well healthy carriers. Methods Oral secretions blood cells from 31 IM, biopsies 14 reactive lymphoid hyperplasia 33 lymphomas were included. was amplified by nested PCR...
Plasmablastic lymphoma (PBL) has been characterised by the World Health Organization as a new entity. This report describes an unusual case of PBL in 3-year-old HIV-infected patient showing cutaneous vulvar lesion with 9 months evolution and prolapsed vulvovaginal mucosa. Histopathological examination biopsy sample showed diffuse submucosal infiltration large cells cohesive growth pattern, round vesicular nuclei fine chromatin centrally or eccentrically placed one more prominent nucleoli....
ABSTRACT The ubiquitous Epstein-Barr virus (EBV) is related to the development of lymphoma and also etiological agent for infectious mononucleosis (IM). Sequence variations in gene encoding LMP1 have been deeply studied different pathologies geographic regions. Controversial results propose existence tumor-related variants, while others argued favor a geographical distribution these variants. Reports assessing EBV variants IM were performed adult patients who displayed multiple variant...
Chemotherapy aims to limit proliferation and induce apoptotic cell death in tumor cells. Owing blockade of signaling pathways involved survival proliferation, nuclear factor κB (NF-κB) inhibitors can apoptosis a number hematological malignancies. The efficacy conventional chemotherapeutic drugs, such as vincristine (VCR) doxorubicine (DOX), may be enhanced with combined therapy based on NF-κB modulation. In this study, we evaluated the effect caffeic acid phenylethyl ester (CAPE) MG-132, two...
Abstract Epstein–Barr virus (EBV) is related to the development of lymphomas and also etiological agent for infectious mononucleosis (IM). Sequence variation EBNA1 gene, consistently expressed in all EBV‐positive cells, has been widely studied. Based on amino acid at codon 487 five major variants have described, two closely prototypic (P‐ala P‐thr) three variant sequences (V‐leu, V‐val, V‐pro). Sub‐variants were then further classified based mutations other than originally described. While...
The sequence variability of the Epstein-Barr virus has been extensively studied throughout previous years in isolates from various geographic regions and consequent variations at both genetic genomic levels have described. However, South America were underrepresented these studies. Here, we sequenced 15 complete EBV genomes that analyzed together with publicly available raw NGS data for 199 other parts globe by means a custom-built bioinformatic pipeline. phylogenetic relations genomes,...
Abstract Epstein–Barr virus genotypes can be distinguished by polymorphic variations in the genes encoding EBNA2, 3A, 3B, and 3C. The immediate early gene BZLF1 plays a key role modulating switch from latency to lytic replication therefore enabling viral propagation. aim of this study was investigate compare promoter sequence (Zp) variation pediatric infectious mononucleosis (IM) EBV positive lymphoma biopsies. Zp sequenced peripheral blood mononuclear cells (PBMC) throat swabs 10 patients...
Objectives: Tumors of the central nervous system (CNS) are most common pediatric solid tumors, where low grade (LGG) and high gliomas (HGG) represent up to 55% CNS tumors. Current molecular classification these tumors results in a more accurate diagnosis risk stratification, which ultimately enables individualized treatment strategies. Identifying known alterations is suitable approach, particularly developing countries, NGS approaches not easily accessible. We sought assess BRAF histone 3...
Most genomic studies on Epstein-Barr virus variability have focused geographic and pathological association of EBV1 genomes. In contrast, the EBV2 genomes has been less explored, mainly due to its restricted circulation lesser number sequenced isolates. this study, we analyzed 28 EBV1, 10 from Argentina, which were combined with 239 downloaded complete other regions, produce an initial multi-sample.vcf file comprised 278 EBV context, identified 1,093/4,541 positions in viral genome that...
T-cell non–Hodgkin's lymphomas (NHLs) represent 10% to 15% of all diagnosed in Western countries. Various geographic frequencies NHL have been documented, part reflecting increased exposure pathogenic factors such as Epstein-Barr virus (EBV). Our aims were assess EBV and p53 expression Argentine pediatric lymphoma correlate them with patients' survival. encoded RNAs (EBERs) situ hybridization LMP1 immunohistochemical staining performed on formalin-fixed paraffin-embedded lymph node biopsies...
Abstract Epstein Barr virus sequence variability has been deeply studied throughout the past years in isolates from various geographic regions and consequent variation at both genetic genomic levels described. However, South America have underrepresented these studies. Here, we sequenced 15 complete EBV genomes that analyzed, by means of a custom-built bioinformatic pipeline, together with publicly available raw NGS data for 199 other parts globe. Phylogenetic relations genomes, structure...