Abstract Intracanal medications are used in endodontic treatment due to their antibacterial activity and ability induce the periapical repair. Among intracanal medications, Calen (CAL; SS. White, Brazil) is a calcium hydroxide-based medication that provides an alkaline pH releases calcium, exerting antimicrobial activity. Bio-C Temp (BIO; Angelus, Brazil), ready-to-use bioceramic medication, was designed stimulate mineralized tissues formation. Here, we investigated bioactive potential of...

To evaluate the effect of calcium hydroxide (CH) associated with two different vehicles as a capping material for pulp tissue in primary molars, compared mineral trioxide aggregate (MTA).

Aim To evaluate the cytotoxicity, biocompatibility and mineralization capacity of BIO-C PULPO, MTA. Methodology L929 fibroblasts were cultured MTT assay was used to determine material cytotoxicity on 6, 24, 48 h. A total 30 male rats (Wistar) aged between 4 6 months, weighing 250 300 g used. Polyethylene tubes containing MTA, empty implanted into dorsal connective tissue. After experimental periods (7, 15, 30, 60, 90 days) histologically analyzed using hematoxylin-eosin (H&E), immunolabeling...

This study evaluated the biocompatibility, bioactivity, porosity, and sealer/dentin interface of Sealer Plus BC (SP), Bio-C (BIOC), TotalFill (TF), AH (AHP). Dentin tubes filled with sealers empty (control group) were implanted in subcutaneous tissue rats for different periods (n = 6 per group/period). Number inflammatory cells (ICs), capsule thickness, von Kossa reaction, interleukin-6 (IL-6) osteocalcin (OCN) evaluated. Porosity voids dentin/sealers assessed by micro-computed tomography....

Abstract Our purpose was to evaluate the biocompatibility and hepatotoxicity of a new bioceramic intracanal medicament, Bio-C Temp (BIO). The biological properties BIO were compared with calcium hydroxide-based medicament (Calen; CAL), used as gold pattern. Polyethylene tubes filled or CAL, empty (control group, CG) implanted into subcutaneous tissue rats. After 7, 15, 30 60 days, samples embedded in paraffin for morphological, quantitative immunohistochemistry analyses. At 7 blood collected...

Abstract Aim To evaluate the biological properties of experimental sealers based on tricalcium silicate and dicalcium silicate, manipulated with polyethylene glycol (CE‐1) addition calcium hypochlorite (CE‐2) compared to AH Plus (AHP) TotalFill BC Sealer (TBC). Methodology The tissue reaction caused by materials in subcutaneous rats was evaluated after implantation tubes filled at 7, 15, 30 60 days. Sections were stained haematoxylin eosin (HE) for morphological analysis number inflammatory...

To evaluate the tissue response promoted by Bio-C Pulpo (Bio), MTA Repair HP (MTA-HP) and White (WMTA) whether these materials cause liver changes in a rat experimental model.Polyethylene tubes filled with Bio, MTA-HP WMTA, empty (control group, CG) were implanted into subcutaneous tissues of rats for 7, 15, 30 60 days. Inflammatory reaction score (IRS), capsule thickness, number inflammatory cells (IC), von Kossa reaction, interleukin-6 (IL-6) alkaline phosphatase (ALP) immunohistochemistry...

Green fluorescent protein (GFP) is a globular used as biosensor and biomarker in medical industrial fields. However, due to the expensive production costs of expressing proteins using high-cost inducers like isopropyl-β-d-1-thiogalactopyranoside (IPTG), number GFP applications are still scarce. This work studied enhanced (EGFP) Escherichia coli BL21 (DE3) [pLysS; pET28(a)], aiming increase its yield reduce costs. First, influence agitation rate, induction time, concentration IPTG EGFP was...

Initial inflammatory response after the pulpotomy of rat molars with MTA or ferric sulfate Purpose: To compare, both qualitatively and quantitatively, cells, vascular density IL-6 immunolabeled cells present in pulp white versus 15.5% (FS).Methodology: Forty-eight mandibular first from 24 Wistar rats were divided into FS groups subdivided according to period procedure (24, 48 72 hours).Four teeth (sound untreated) used as controls.Histological sections obtained assessed through descriptive...

Abstract As the biocompatibility and bioactive potential of repair materials are desired characteristics in dentistry, tissue response Bio‐C Pulpo, a bioceramic material launched on marked by Angelus (Brazil), was compared with Biodentine (Septodont, France) White MTA (WMTA; Angelus, Brazil). In 32 rats, 148 polyethylene tubes filled or WMTA, empty (CG, control group) were implanted into subcutaneous tissues for 7, 15, 30, 60 days. The capsule thickness, numerical density inflammatory cells...

Abstract Several synthetic and natural materials have been studied for the confection of temporary grafts application in regenerative medicine, however, development a material with adequate properties remains challenge, mainly because its degradation kinetics biological systems. Nature provides noble that can be used as such many applications, thus, taking advantage available morphology assembled structures plants, we propose to study vegetable stems use graft. Since vivo is maybe one most...

To evaluate whether the bioceramic materials Bio-C Pulpo (Bio-C, Angelus) and mineral trioxide aggregate (MTA) Repair HP (MTA-HP, induce fibroblast proliferation release of interleukin-10 (IL-10), an anti-inflammatory cytokine, stimulating connective tissue remodelling. The response MTA-HP was compared with White MTA (WMTA; since studies have demonstrated that WMTA induces repair.Bio-C, were inserted into polyethylene tubes implanted in subcutaneous Holtzman rats for 7, 15, 30 60 days. As a...

Our purpose was to investigate the physicochemical properties of Bio-C Temp, a bioceramic intracanal medication, and whether its residues remain adhered dentine walls after conventional manual irrigation (CMI) or passive ultrasonic (PUI) in comparison Calen.

A Harmonização Orofacial foi reconhecida pelo Conselho Federal de Odontologia como uma nova especialidade. Esta pesquisa teve objetivo analisar as implicações éticas e legais da atuação cirurgiões-dentistas em harmonização orofacial. Para isso, realizada revisão literatura sobre normativas diretrizes relacionadas à prática, bem análise jurisprudência a partir processos judiciais pertinentes. Além disso, foram considerados dados fornecidos por instituições classe relevantes, Sociedade...

This study aimed to survey and analyze the profile of experimental dental research in animals, verifying its trends. We evaluated studies developed with use animals vivo, published 10 journals high impact factors, from 2015 2020. From 1652 retrieved, 594 involved vivo experimentation on were analyzed further. Rats species most used highest mean per study. Ferrets, although presenting lowest rate publications, had second Periodontics was specialty number while oral rehabilitation number. The...

O uso excessivo de antibióticos e o aparecimento bactérias resistentes são uma preocupação global. Essa também é importante em termos microbiota a utilização para lidar com as infecções dentárias. As odontogênicas, incluindo endodônticas, provocadas por polimicrobianos que envolvem combinação gram-positivos, gram-negativos, anaeróbios facultativos anaeróbias estritas.

Endodontic retreatment continues to be a challenge for Endodontics, as well supporting techniques that aim improve and assist in the elimination of microorganisms have infected root canal systems led development peripicopathies, such Passive Ultrasonic Irrigation (PUI) Photodynamic Antimicrobial Therapy (aPDT) been frequently used. Thus, this paper is report clinical case endodontic tooth 47 with diagnosis endoperiodontal injury associated periapical lesion. The treatment was carried out two...