A5038568757- Marine Ecology and Invasive Species
- Planarian Biology and Electrostimulation
- bioluminescence and chemiluminescence research
- Telomeres, Telomerase, and Senescence
- Cellular Mechanics and Interactions
- Cell Adhesion Molecules Research
- melanin and skin pigmentation
- Plant and Biological Electrophysiology Studies
- Epigenetics and DNA Methylation
- Melanoma and MAPK Pathways
- Genomics and Chromatin Dynamics
- T-cell and B-cell Immunology
- Skin and Cellular Biology Research
- Biochemical Analysis and Sensing Techniques
- Immunotherapy and Immune Responses
- Skin Protection and Aging
Cancer Research UK Scotland Institute
2017-2022
University of the West of Scotland
2020-2022
University of Glasgow
2016-2022
Switch
2017
University of Oxford
2013
University of Nottingham
2012
Institut thématique Génétique, génomique et bioinformatique
2012
Queen's Medical Centre
2012
Abstract Background Planarian stem cells, or neoblasts, drive the almost unlimited regeneration capacities of freshwater planarians. Neoblasts are traditionally described by their morphological features and fact that they only proliferative cell type in asexual Therefore, can be specifically eliminated irradiation. Irradiation, however, is likely to induce transcriptome-wide changes gene expression not associated with neoblast ablation. This has affected accurate description specific...
In most sexually reproducing animals, replication and maintenance of telomeres occurs in the germ line during early development embryogenesis through use telomerase. Somatic cells generally do not maintain telomere sequences, these become senescent adults as shorten to a critical length. Some animals reproduce clonally must therefore require adult somatic mechanisms for maintaining their chromosome ends. Here we study biology planarian flatworms with apparently limitless regenerative...
Histone modification H4K20me3 and its methyltransferase SUV420H2 have been implicated in suppression of tumorigenesis. The underlying mechanism is unclear, although abundance increases during cellular senescence, a stable proliferation arrest tumor suppressor process, triggered by diverse molecular cues, including activated oncogenes. Here, we investigate the function senescence suppression. Using immunofluorescence ChIP-seq determine distribution proliferating senescent human cells. Altered...
Currently, little is known about the evolution of epigenetic regulation in animal stem cells. Here we demonstrate, using planarian cell system to investigate role COMPASS family MLL3/4 histone methyltransferases that their function as tumor suppressors mammalian cells conserved over a long evolutionary distance. To potential conservation genome-wide regulatory program cells, assess effects Mll3/4 loss by performing RNA-seq and ChIP-seq on G2/M population, part which contributes formation...
Planarian adult stem cells (pASCs) or neoblasts represent an ideal system to study the evolution of and pluripotency as they underpin unrivaled capacity for regeneration. We wish understand control differentiation in pASCs how conserved, convergent divergent these mechanisms are across Bilateria. Here we show planarian methyl-CpG Binding Domain 2/3 (mbd2/3) gene is required pASC during regeneration tissue homeostasis. The genome does not have detectable levels 5-methylcytosine (5mC) find no...
On acquisition of an oncogenic mutation, primary human and mouse cells can enter oncogene-induced senescence (OIS). OIS is characterized by a stable proliferation arrest secretion proinflammatory cytokines chemokines, the senescence-associated secretory phenotype. Proliferation phenotype collaborate to enact tumor suppression, former blocking cell latter recruiting immune clear damaged cells. However, interactions with system are still poorly defined. Here, we show that engagement in...
The Arp2/3 complex is essential for the assembly of branched filamentous actin but its role in physiology and development surprisingly little understood. Melanoblasts deriving from neural crest migrate along developing embryo traverse dermis to reach epidermis colonising skin eventually homing within hair follicles. We have previously established that Rac1 Cdc42 direct melanoblast migration vivo. hypothesised might be main downstream effector these small GTPases. Arp3 depletion melanocyte...
Abstract Currently, little is known about the evolution of epigenetic regulation in animal stem cells. Using planarian cell system to investigate role COMPASS family MLL3/4 histone methyltransferases, we demonstrate that their as tumour suppressors cells conserved over a large evolutionary distance animals. This also suggested potential conservation genome wide program cells, so assessed regulatory effects Mll3/4 loss function by performing RNA-seq and ChIP-seq on G2/M population, part which...
Abstract Histone chaperone HIRA is thought to play a role in both early development and aging, but little known about connections between the two processes. Here, we explore this relationship using lineage-specific knockout mouse model, TyrCre::Hira fl/fl , which deficient pigmentary system consisting of embryonic melanoblasts, postnatal melanocytes melanocyte stem cells (McSCs). Hira leads reduced melanoblast numbers during embryogenesis, wild type at birth, normally functioning juvenile...