A5052454334- Pregnancy and preeclampsia studies
- Microbial Metabolic Engineering and Bioproduction
- RNA and protein synthesis mechanisms
- Reproductive System and Pregnancy
- Bacterial Genetics and Biotechnology
- Birth, Development, and Health
- Gestational Diabetes Research and Management
- Pregnancy and Medication Impact
- Prenatal Screening and Diagnostics
- Maternal and fetal healthcare
- Assisted Reproductive Technology and Twin Pregnancy
- Fibroblast Growth Factor Research
- Proteoglycans and glycosaminoglycans research
- Enzyme Catalysis and Immobilization
- Angiogenesis and VEGF in Cancer
- Eosinophilic Esophagitis
- Microbial Natural Products and Biosynthesis
- CRISPR and Genetic Engineering
- X-ray Diffraction in Crystallography
- Maternal and Perinatal Health Interventions
- Acute Ischemic Stroke Management
- Electrolyte and hormonal disorders
- Cardiovascular Syncope and Autonomic Disorders
- Parathyroid Disorders and Treatments
- Crystallization and Solubility Studies
University of Manchester
2013-2024
University of Florida
2019-2024
Beckman Laser Institute and Medical Clinic
2021-2024
University of California, Irvine
2021-2024
Louisiana State University
2021-2024
Florida College
2019-2022
Institute for Neurodegenerative Disorders
2022
Seqirus (United States)
2022
Allen Institute for Brain Science
2022
University of Kansas Medical Center
2021
The microbial production of fine chemicals provides a promising biosustainable manufacturing solution that has led to the successful growing catalog natural products and high-value chemicals. However, development at industrial levels been hindered by large resource investments required. Here we present an integrated Design-Build-Test-Learn (DBTL) pipeline for discovery optimization biosynthetic pathways, which is designed be compound agnostic automated throughout. We initially applied...
The field of synthetic biology aims to make the design biological systems predictable, shrinking huge space practical numbers for testing. When designing microbial cell factories, most optimization efforts have focused on enzyme and strain selection/engineering, pathway regulation, process development. In silico tools predictive bacterial ribosome binding sites (RBSs) RBS libraries now allow translational tuning biochemical pathways; however, methods predicting optimal combinations in...
It is well known that a variety of inflammatory diseases are accompanied by hypercoagulability, and number more-or-less longer-term signalling pathways have been shown to be involved. In recent work, we suggested direct primary role for bacterial lipopolysaccharide (LPS) in this but it seems never tested directly. Here, show the addition tiny concentrations (0.2 ng l −1 ) LPS both whole blood platelet-poor plasma normal, healthy donors leads marked changes nature fibrin fibres so formed, as...
The vascular endothelial growth factor (VEGF) family of proteins controls the formation and blood vessels. most potent widely expressed isoform, VEGF165, is secreted as a disulfide-linked homodimer with two identical heparin-binding sites. Interactions heparan sulfate (HS) regulate diffusion, half-life, affinity VEGF165 for its signaling receptors. We have determined number key HS structural features that mediate specific binding dimer. Carboxylate groups 2-O-, 6-O-, N-sulfation contributed...
Abstract Objective . To determine if pregnancy complications are increased in super‐obese (a body mass index (BMI) of 50 or more) compared to other, less obese parturients. Design Cross‐sectional study. Setting and population All 19,700 eligible women, including 425 (2.2%) women with singleton births between 1996 2007 delivering at a tertiary referral center, identified using perinatal research database. Methods Bivariate trend analyses were used assess the relation super‐obesity various...
Ligand-dependent control of gene expression is essential for functional analysis, target validation, protein production, and metabolic engineering. However, the tools currently available are difficult to transfer between species exhibit limited mechanistic diversity. Here we demonstrate how modular architecture purine riboswitches can be exploited develop orthogonal chimeric switches that transferable across diverse bacterial species, modulating either transcription or translation, provide...
Abstract Natural plant-based flavonoids have drawn significant attention as dietary supplements due to their potential health benefits, including anti-cancer, anti-oxidant and anti-asthmatic activities. Naringenin, pinocembrin, eriodictyol homoeriodictyol are classified (2S)-flavanones, an important sub-group of naturally occurring flavonoids, with wide-reaching applications in human nutrition. These four compounds occupy a central position branch point intermediates towards broad spectrum...
Bio-based production of industrial chemicals using synthetic biology can provide alternative green routes from renewable resources, allowing for cleaner processes. To efficiently produce on-demand through microbial strain engineering, biomanufacturing foundries have developed automated pipelines that are largely compound agnostic in their time to delivery. Here we benchmark the capabilities a pipeline enable rapid prototyping cell factories chemically diverse industrially relevant material...
Heparin-like saccharides play an essential role in binding to both fibroblast growth factors (FGF) and their receptors at the cell surface. In this study we prepared a series of heparin oligosaccharides according size sulfation level. We then investigated affinity for FGF2 ability support mitogenesis heparan sulfate-deficient cells expressing FGFR1c. Tetra- hexasaccharides bound FGF2, but failed dimerize factor. Nevertheless, these promoted FGF2-mediated growth. Furthermore, whereas...
Synthetic biology utilizes the Design-Build-Test-Learn pipeline for engineering of biological systems. Typically, this requires construction specifically designed, large and complex DNA assemblies. The availability cheap synthesis automation enables high-throughput assembly approaches, which generates a heavy demand sequencing to verify correctly assembled constructs. Next-generation is ideally positioned perform task, however with expensive hardware costs bespoke data analysis requirements...
The binding of Zn(II) cations to a pentafoil (51) knotted ligand allows the synthesis otherwise inaccessible metalated molecular knots via transmetalation, affording corresponding "first-sphere" coordination Co(II), Ni(II), and Cu(II) pentanuclear in good yields (≥85%). Each knot complexes was characterized by mass spectrometry, diamagnetic (zinc) complex 1H 13C NMR spectroscopy, zinc, cobalt, nickel afforded single crystals whose structures were determined X-ray crystallography. Lehn-type...
The related glycosaminoglycans heparin and heparan sulfate are essential for the activity of fibroblast growth factor (FGF) family as they form an integral part signaling complex at cell surface. Using size-exclusion chromatography we have studied capacities a variety oligosaccharides to bind FGF1 FGFR2c both separately together in ternary complexes. In absence heparin, had no detectable affinity FGFR2c. However, 2:2:1 complexes formed spontaneously solution between FGF1, FGFR2c,...