A5081220309- Enzyme Structure and Function
- X-ray Spectroscopy and Fluorescence Analysis
- Protein Structure and Dynamics
- Advanced X-ray Imaging Techniques
- Cardiomyopathy and Myosin Studies
- Advanced Electron Microscopy Techniques and Applications
- Electron and X-Ray Spectroscopy Techniques
- Muscle Physiology and Disorders
- Biochemical and Molecular Research
- Crystallography and Radiation Phenomena
- Mass Spectrometry Techniques and Applications
- Cardiovascular Effects of Exercise
- Particle Accelerators and Free-Electron Lasers
- Engineering Applied Research
- Advanced MRI Techniques and Applications
- Nuclear Physics and Applications
- Heat Transfer and Boiling Studies
- Genetics, Bioinformatics, and Biomedical Research
- Cellular Mechanics and Interactions
- Particle Detector Development and Performance
- Heat shock proteins research
- Cell Image Analysis Techniques
- Radioactive Decay and Measurement Techniques
- Collagen: Extraction and Characterization
- Microstructure and Mechanical Properties of Steels
Argonne National Laboratory
2005-2024
Lawrence Berkeley National Laboratory
2019
University of Georgia
2004-2017
Advanced Photon Source
2005-2017
Heidelberg University
2016
Oregon Health & Science University
2011
Diamond Light Source
2011
Joint Center for Structural Genomics
2008
Czech Academy of Sciences, Institute of Physics
2005
Institute of Biostructure and Bioimaging
2002
Radiation damage is a major limitation in crystallography of biological macromolecules, even for cryocooled samples, and particularly acute microdiffraction. For the X-ray energies most commonly used protein at synchrotron sources, photoelectrons are predominant source radiation damage. If beam size small relative to photoelectron path length, then may escape footprint, resulting less illuminated volume. Thus, it be possible exploit this phenomenon reduce radiation-induced during data...
The 19ID undulator beamline of the Structure Biology Center has been designed and built to take full advantage high flux, brilliance quality X-ray beams delivered by Advanced Photon Source. optics are capable delivering monochromatic X-rays with photon energies from 3.5 20 keV (3.5–0.6 Å wavelength) fluxes up 8–18 × 1012 photons s−1 (depending on energy) onto cryogenically cooled crystal samples. size beam (full width at half-maximum) sample position can be varied 2.2 mm 1.0 (horizontal...
The 18ID undulator beamline of the Biophysics Collaborative Access Team at Advanced Photon Source, Argonne, IL, USA, is a high-performance instrument designed for, and dedicated to, study partially ordered disordered biological materials using techniques small-angle X-ray scattering, fiber diffraction, absorption spectroscopy. associated instrumentation are described in detail examples representative experimental results presented.
The use of synchrotron radiation as an intense X-ray source for biological diffraction has only very recently been promoted into a long-term experimental programme. Here we report on some general and detailed aspects this development. In the case small-angle studies weakly scattering specimens at high time resolution, e.g. stroboscopic muscle, lack sources considerably higher brightness than from conventional generators become limiting. As it technically impossible to pursue development...
Some years ago Reedy, Holmes, and Tregear (1965) noted that ATP had a dramatic effect upon the low-angle X-ray diffraction pattern of gly-cerinated fibers flight muscle from giant water bug Lethocerus maximus. A strong meridional reflection appeared at spacing 145 in presence ATP. When was washed out, this disappeared its place taken by 380 layer line. With help electron microscopy these changes were shown to be due an alteration orientation cross-bridges. In absence (rigor state)...
Cryocooling is a technique routinely used to mitigate the effects of secondary radiation damage on macromolecules during X-ray data collection. Energy from beam absorbed by sample raises temperature sample. How large increase and does this reduce effectiveness cryocooling? Sample heating has been measured non-invasively for first time means thermal imaging. Specifically, rise 1 mm 2 glass spheres (sample surrogates) exposed an intense synchrotron cooled in laminar flow nitrogen gas...
The polarization of extreme uv light provided by a normal incidence monochromator used with synchrotron radiation from the 6-GeV electron DESY has been measured. measured increases 82% at 500 Å to 87% 1000 Å, and is in excellent agreement calculated incident upon grating. A reflecting polarimeter consisting polarizer an analyzer each made four gold mirrors measurement.
Background: The fragile histidine triad (FHIT) protein is a member of the large and ubiquitous (HIT) family proteins. It expressed from gene located at site on human chromosome 3, which commonly disrupted in association with certain cancers. On basis genetic evidence, it has been postulated that FHIT may function as tumor suppressor, implying role for carcinogenesis. dinucleoside polyphosphate hydrolase activity vitro, thus suggesting its vivo involve hydrolysis phosphoanhydride bond....
The Structural Biology Center beamline, 19ID, has been designed to take full advantage of the highly intense undulator radiation and very low source emittance available at Advanced Photon Source. In order keep X-ray beam focused onto pre-sample slits, a novel position-sensitive PIN diode array developed. consists four diodes positioned upstream 0.5 microm-thick metal foil placed in beam. Using conventional difference-over-the-sum techniques, two-dimensional position information is obtained...
Radiation damage is an unavoidable obstacle in X-ray crystallographic data collection for macromolecular structure determination, so it important to know how much radiation a sample can endure before being degraded beyond acceptable limit. In the literature, threshold at which average intensity of all recorded reflections decreases certain fraction initial value called `dose limit'. The first estimated D50 dose-limit value, diffracted was reduced 50%, 20 MGy and derived from observing decay...
Ultrahigh-resolution structures provide unprecedented details about protein dynamics, hydrogen bonding and solvent networks. The reported 0.70 Å, room-temperature crystal structure of crambin is the highest-resolution ambient-temperature a achieved to date. Sufficient data were collected enable unrestrained refinement associated networks using SHELXL . Dynamic resulting from alternative side-chain conformations shifts in water positions are revealed, demonstrating that polypeptide...
Mild cognitive impairment (MCI) is a frequent syndrome in the older population, which involves an increased risk to develop Alzheimer's disease (AD). The latter can be modified by reserve, operationalized length of school education. MCI differentiated into four subtypes according domains involved: amnestic MCI, multiple-domain non-amnestic and MCI. While neurocognitive deficits are constituent diagnosis these subtypes, question how they refer reserve still needs clarified.We examined...
Serial synchrotron crystallography enables the study of protein structures under physiological temperature and reduced radiation damage by collection data from thousands crystals. The Structural Biology Center at Sector 19 Advanced Photon Source has implemented a fixed-target approach with new 3D-printed mesh-holder optimized for sample handling. holder immobilizes crystal suspension or droplet emulsion on nylon mesh, trapping sealing near-monolayer crystals in its mother liquor between two...
The need to record low-angle-scattering X-ray fibre diagrams from muscle with millisecond time resolution drove the use of synchrotron radiation as an light source. first smudgy diffraction patterns were obtained a slice insect flight muscle. Out this grew EMBL Outstation at DESY.