A5052029027- Vector-borne infectious diseases
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Vector-Borne Animal Diseases
- Plant Virus Research Studies
- Yersinia bacterium, plague, ectoparasites research
- Insect and Pesticide Research
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Bartonella species infections research
- Livestock and Poultry Management
- Genomics and Phylogenetic Studies
- Bird parasitology and diseases
- Viral gastroenteritis research and epidemiology
- Plant Parasitism and Resistance
State Research Center of Virology and Biotechnology VECTOR
2011-2024
Genetic Technologies (Australia)
2020-2023
Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
2022
In this work we tested two reagent kits developed by us for detecting SARS-CoV-2 RNA using a fragment of the ORF1ab gene in digital PCR and real-time formats. Data were obtained on detection virus nasopharyngeal swabs patients with COVID-19 asymptomatic carriers. The provided 100% sensitivity limit 103 GE / ml qPCR, at least 200 copies viral when performing PCR. These methods panel 1,328 samples collected from suspected beginning 2020 Russian Federation. It has been shown that dPCR is more...
Novel Haseki tick virus (HSTV) was detected in ixodid ticks and patients the Asian part of Russia. Sequencing genome fragments corresponding whole polyprotein viral RdRp demonstrated that HSTV is genetically close to unclassified Flavi-like viruses. Phylogenetic analysis sequences showed these viruses were Bole 4 (BLTV 4), which early Asia, Europe, Africa Caribbean region. The organization predicts BLTV may also be classified as putative new genera within Flaviviridae with enlarged...
The objectives of our study were to survey the prevalence genetic markers for Rickettsia spp., Ehrlichia Anaplasma Babesia and Theileria spp. in Hyalomma anatolicum ticks collected southwestern Tajikistan perform sequencing phylogenetic analysis fragments 16S rRNA gene groESL ope-ron from 18S detected H. ticks. Tursunzade Rudaki districts tested DNA by PCR with specific primers. amplified sequenced analyzed. (3.3 %) was only district, (0.7 found district. Sequence operon revealed high...
Determined is the complete genome sequence of Kolarovo-2008 strain (Siberia subtype) Tick-borne encephalitis virus (TBEV), isolated from a tick in suburbs Tomsk city. Nucleotide analysis testifies fact that level genetic differences within Siberian subtype TBEV amounts to 10 % nucleotide and 7 amino-acid for certain genes. 3'-HTO has highest rate variability homology ranging 65 97 %. Vasilchenko (isolated Novosibirsk 1969) strains have homology. The dissimilarity between two substantially...
From 2006 to 2011, in the Tomsk region (south of Western Siberia), eight species pathogens were detected birds and ticks feeding on them: Tick-borne encephalitis virus (TBEV), West Nile (WNV), Borrelia spp., Rickettsia Bartonella Anaplasma Ehrlichia Babesia spp. The identification a number strains viruses bacterial genovariants related geographically with Russian Far East, Eastern Siberia, China Japan confirms possibility role spread direction Siberia back. Most that breed migrate are origin...
The south of Western Siberia traditionally belongs to the regions with unfavorable epidemiological situation for tick-transmitted infections. In Novosibirsk and its suburbs, approximately 20 thousand people annually seek medical care after tick bites. purpose this work was determine human-attacking species composition in territory suburbs by identifying nucleotide sequence mitochondrial gene cytochrome oxidase COI fragment during 2018 spring-summer season, determination infections individual...
This paper reports the analysis of nucleotide sequences 5'-untranslated region (5'-UTR) tick-borne encephalitis virus (TBEV) genomic RNA isolated from 39 individual taiga ticks collected in several regions Northern Eurasia. The 5'-UTRs Siberian and Far East TBEV genotypes were 89% 95% identical to prototype strains (Zausaev 205), respectively. detected substitutions typical for these two genotypes, which made possible unambiguous identification. Both conservative variable motifs 5'-UTR RNA....
The tick-borne encephalitis virus (TBEV) strain C11-13 (GenBank acc. no. OQ565596) of the Siberian genotype was previously isolated from brain a deceased person. TBEV variants obtained at passages 3 and 8 in SPEV cells were inoculated into brains white mice for subsequent passages. Full genome sequences all analyzed by high-throughput sequencing. A total 41 single nucleotide substitutions found to occur mainly genes nonstructural proteins NS3 NS5 MF043953, OP902894, OP902895), 12 amino acid...
Objective of the study was to assess level infection with Borrelia miyamotoi in ticks Ixodes persulcatus and pavlovskyi collected south Western Siberia. Materials methods. 688 I. on territory Novosibirsk, Tomsk Kemerovo Regions were examined. DNA detected by a two-round polymerase chain reaction specifc primers omp66 gene region, followed sequencing isolated fragments phylogenetic analysis, including sequences prototype isolates published international GenBank database. Comparison analysis...
Here, we present the complete mitochondrial DNA sequence of Dermacentor reticulatus. The mitogenome is 14,806 bp and contains 13 protein-coding, 2 rRNA, 22 tRNA genes, along with control regions. reticulatus has common gene order Metastriata ticks. It phylogenetically close to mitogenomes ticks, which D. everestanus closest 85.7% similarity. These data provide insights into phylogenetic relations among
Extensive spread of tick-borne diseases poses a significant problem for public health and the population living in endemic areas. The aim study was to search, analyze genetic material identify new viral agents Phenuiviridae family taiga ticks collected Asian regions Russia using method high throughput sequencing. Materials methods. involved 1460 suburban areas Tomsk, Yekaterinburg Primorsky Territory. isolated from sequenced Illumina technology followed by phylogenetic analysis. Results...
Cases of detection RNA or ELISA markers West Nile virus (WNV) have been recorded in many countries Southeast Asia. At the same time, there were no reports outbreaks fevers caused by WNV. Perhaps this is due to asymptomatic very mild course disease focus health systems these on other viral fevers. In work, for first time Vietnam, was detected poultry. WNV found three lung samples chickens from Nghe An province collected 2017. The isolates belonged lineages 1a and 2, responsible Europe North...