A5066556367- Viral Infectious Diseases and Gene Expression in Insects
- Insect Resistance and Genetics
- Hippo pathway signaling and YAP/TAZ
- Invertebrate Immune Response Mechanisms
- Virus-based gene therapy research
- Cellular transport and secretion
- Neurobiology and Insect Physiology Research
- Phagocytosis and Immune Regulation
- Entomopathogenic Microorganisms in Pest Control
- RNA modifications and cancer
- Cancer Cells and Metastasis
- Coal and Its By-products
- CRISPR and Genetic Engineering
- Thermochemical Biomass Conversion Processes
- Insect symbiosis and bacterial influences
- Catalytic Processes in Materials Science
- RNA Research and Splicing
- Cell Adhesion Molecules Research
- Fibroblast Growth Factor Research
- Entomological Studies and Ecology
- Pancreatic and Hepatic Oncology Research
- Cancer-related gene regulation
- Coal Combustion and Slurry Processing
- Heat shock proteins research
- Wnt/β-catenin signaling in development and cancer
Xi'an Jiaotong University
2024
University of Massachusetts Chan Medical School
2013-2022
Agriculture and Agri-Food Canada
2010-2017
Summerland Research and Development Centre
2014
Chinese Academy of Sciences
2001-2011
Research Center for Eco-Environmental Sciences
2011
Ministry of Ecology and Environment
2011
University of British Columbia
2008-2010
Wuhan Institute of Virology
2005-2006
Central China Normal University
2005
Intestinal stem cells in the adult Drosophila midgut are regulated by growth factors produced from surrounding niche including enterocytes and visceral muscle. The role of other major cell type, secretory enteroendocrine cells, regulating intestinal remains unclear. We show here that newly eclosed scute loss-of-function mutant flies completely devoid cells. These cell-less have normal ingestion fecundity but shorter lifespan. Moreover, these flies, diet-stimulated depends on insulin-like...
Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac96 is a core gene, but its role in virus replication still unknown. To determine the baculovirus life cycle, we used AcMNPV bacmid system to generate an ac96-null (vAc(96)(null)). Our analyses showed that absence of does not affect budded (BV) production or viral DNA infected Sf9 cells. Western blotting and confocal immunofluorescence analysis AC96 expressed both cytoplasm nucleus throughout infection. In addition, was detected...
Abstract Compartmentalization of Toll‐like receptors ( TLR s) in intestinal epithelial cells IEC regulates distinct immune responses to microbes; however, the specific cellular machinery that controls this mechanism has not been fully identified. Here we provide genetic evidences recycling endosomal compartment enterocytes maintains a homeostatic 9 intracellular distribution, supporting mucosal tolerance normal microbiota. Genetic ablation endosome resident small GTP ase, Rab11a , gene...
Autographa californica multiple nucleopolyhedrovirus ac68 is a core gene that overlaps lef3 which encodes the single-stranded DNA binding protein. A knockout (KO) virus lacking both and was generated (lef3-ac68 2×KO) to enable functional study of ac68. To produce an ac68KO did not impact expression, lef3-ac68 2×KO repaired with fragment containing ac68, in contained point mutations so only LEF3 expressed. Repair just lef3KO virus. Analysis showed viral replication budded (BV) levels were...
This study assessed the potential of ozone for degradation nine commonly used veterinary antibiotics (five sulfonamides, three tetracyclines, and one macrolide) in swine wastewater pretreated by sequencing batch reactor (SBR). The apparent second-order rate constants reactions selected toward Milli-Q water (kO3 at pH 7.0 T=20°C) were determined competition kinetics as follows: sulfonamides [(1.9–6.2)×106 M-1s-1], tetracyclines (6.9×106 to 1.7×107 M-1s-1), tiamulin (3.8×105 M-1s-1). could be...
Abstract The effects of polarized membrane trafficking in mature epithelial tissue on cell growth and cancer progression have not been fully explored vivo. A majority colorectal cancers reduced mislocalized Rab11, a small GTPase dedicated to recycling endosomes. Patients with low Rab11 protein expression poor survival rates. Using genetic models across species, we show that intact endosome function restrains aberrant elicited by APC or RAS mutations. Loss led dysplasia early animal...
Drosophila Toll-1 and all mammalian Toll-like receptors regulate innate immunity. However, the functions of remaining eight Toll-related proteins in are not fully understood. Here, we show that Toll-9 is necessary sufficient for a special form compensatory proliferation after apoptotic cell loss (undead apoptosis-induced [AiP]). Mechanistically, AiP, interacts with to activate intracellular pathway nuclear translocation NF-κB-like transcription factor Dorsal, which induces expression...
The Toll signaling pathway has a highly conserved function in innate immunity and is regulated by multiple factors that fine tune its activity. One such factor β-arrestin Kurtz (Krz), which we previously implicated the inhibition of developmental Drosophila melanogaster embryo. Another level controlling activity immune system homeostasis protein sumoylation. In this study, have uncovered link between these two modes regulation show Krz affects sumoylation via interaction with SUMO protease,...
Open reading frame 132 (Ha132) of Helicoverpa armigera nucleopolyhedrovirus (HearNPV) is a homologue per os infectivity factor 2 (pif-2) Spodoptera exigua multiple nucleopolyhedrovirus. Sequence analysis indicated that Ha132 encoded protein 383 aa with predicted molecular mass 44.5 kDa. Alignment HA132 and its baculovirus homologues revealed was highly conserved among baculoviruses, 14 absolutely cysteine residues. RT-PCR first transcribed at 24 h post-infection. Western blot showed 43 kDa...
During the infection cycle of Autographa californica multiple nucleopolyhedrovirus (AcMNPV), two forms virions are produced, budded virus (BV) and occlusion-derived (ODV). Nucleocapsids that form BV have to egress from nucleus, whereas nucleocapsids ODV remain inside nucleus. The molecular mechanism determines whether or nucleus is unknown. AC141 (a predicted E3 ubiquitin ligase) viral (vUbi) both been shown be required for efficient production. In this study, it was hypothesized vUbi...
Efficient manufacturing of recombinant adenovirus-associated virus (AAV) vectors is critical to the successful development genomic medicines. We attempted optimize AAV vector production in a producer cell line platform. In this system, helper functions required for replication and are provided via infection with replication-competent wild-type Adenovirus. To evaluate strategies reduction packaging adenovirus understand interplay during production, was compared mutant (Ad5ts149) containing...
Intestinal stem cells (ISCs) in the adult Drosophila midgut can respond to tissue damage and support repair. We used genetic manipulation increase number of ISC-like performed gene expression profiling identify potential ISC regulators. A detailed analysis one these regulators, zinc-finger protein Charlatan, was carried out. MARCM clonal RNAi precursor showed that loss Chn function caused severe division defects, including EdU incorporation, phosphorylated histone 3 staining mitotic Cdc2....
Abstract Colorectal cancer is a complex disease driven by well-established mutations such as APC and other yet to be identified pathways. The GTPase Rab11 regulates endosomal protein trafficking, previously we showed that loss of caused intestinal inflammation hyperplasia in mice flies. To test the idea may promote progression, have analyzed archival human patient tissues observed 51 out 70 colon had lower staining. By using Drosophila midgut model, found can lead three changes relate...
Abstract The intestinal epithelium has a high rate of cell turn over and is an excellent system to study stem cell‐mediated tissue homeostasis. Misshapen subfamily the Ste20 kinases in mammals consists misshapen like kinase 1 (MINK1), mitogen‐activated protein 4 (MAP4K4), TRAF2 NCK interacting (TNIK). Recent reports suggest that this novel function equal Hippo/MST as upstream for Warts/Large tumor suppressor (LATS) suppress growth. To vivo functions Mink1, Map4k4 , Tnik we generated compound...