A5080609288- Diet, Metabolism, and Disease
- Diet and metabolism studies
- Liver Disease Diagnosis and Treatment
- Metabolism, Diabetes, and Cancer
- Gut microbiota and health
- Sperm and Testicular Function
- Digestive system and related health
- Pancreatic function and diabetes
- Hormonal and reproductive studies
- Dietary Effects on Health
- Reproductive biology and impacts on aquatic species
- Birth, Development, and Health
- Tryptophan and brain disorders
- Animal Nutrition and Physiology
- Fatty Acid Research and Health
- Endoplasmic Reticulum Stress and Disease
- Clinical Nutrition and Gastroenterology
- Infant Nutrition and Health
- Escherichia coli research studies
- Gastrointestinal motility and disorders
- Vitamin D Research Studies
- Adipose Tissue and Metabolism
- Stress Responses and Cortisol
- Eating Disorders and Behaviors
- Regulation of Appetite and Obesity
Microbiologie de l’alimentation au service de la santé
2017-2025
AgroParisTech
2017-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2025
Université Paris-Saclay
2017-2025
Institut National de la Recherche Agronomique d'Algérie
2019
Institut National de la Recherche Agronomique
2003-2019
Rutgers, The State University of New Jersey
2007-2016
Rutgers New Jersey Medical School
2007-2016
Rutgers Sexual and Reproductive Health and Rights
2014
Rutgers Health
2013
The constant self renewal and differentiation of adult intestinal stem cells maintains a functional mucosa for lifetime. However, the molecular mechanisms that regulate cell division epithelial homeostasis are largely undefined. We report here small GTPases Cdc42 Rab8a critical regulators these processes in mice. Conditional ablation mouse epithelium resulted formation large intracellular vacuolar structures containing microvilli (microvillus inclusion bodies) enterocytes, phenotype...
Dietary fructose that is linked to metabolic abnormalities can up-regulate its own absorption, but the underlying regulatory mechanisms are not known. We hypothesized glucose transporter (GLUT) protein, member 5 (GLUT5) primary and absorption via GLUT5, metabolism ketohexokinase (KHK), as well GLUT5 trafficking apical membrane Ras-related protein-in-brain 11 (Rab11)a-dependent endosomes each required for regulation. Introducing lysine solutions into lumen increased by 2- 10-fold...
Marked increases in fructose consumption have been tightly linked to metabolic diseases. One-third of ingested is metabolized the small intestine, but underlying mechanisms regulating expression fructose-metabolizing enzymes are not known. We used genetic mouse models test hypothesis that absorption via glucose transporter protein, member 5 (GLUT5), metabolism ketohexokinase (KHK), as well GLUT5 trafficking apical membrane Ras-related protein brain 11a (Rab11a)-dependent endosomes required...
Obesity is often associated with sex-dependent metabolic complications, in which altered intestinal barrier function and gut microbiota contribute. We aimed to characterize mice the effects of a high fat diet on these parameters. Male female C57BL/6 received standard (SD) or (HFD; 60% kcal from fat) during 14 weeks (W14). Body composition, glucose tolerance, insulin sensitivity, permeability, colonic expression 44 genes encoding factors involved inflammatory response function, cecal...
Abstract Background Based on the observation of an increased number paralogous genes in teleost fishes compared with other vertebrates and conserved synteny between duplicated copies, it has been shown that a whole genome duplication (WGD) occurred during evolution Actinopterygian fish. Comparative phylogenetic dating this event suggests early on, specifically teleosts. It proposed might have facilitated evolutionary radiation phenotypic diversification fish, notably by allowing sub- or...
Abstract Compartmentalization of Toll‐like receptors ( TLR s) in intestinal epithelial cells IEC regulates distinct immune responses to microbes; however, the specific cellular machinery that controls this mechanism has not been fully identified. Here we provide genetic evidences recycling endosomal compartment enterocytes maintains a homeostatic 9 intracellular distribution, supporting mucosal tolerance normal microbiota. Genetic ablation endosome resident small GTP ase, Rab11a , gene...
Elevated blood fructose concentrations constitute the basis for organ dysfunction in fructose-induced metabolic syndrome. We hypothesized that diet-induced changes are regulated by ketohexokinase (KHK) and transporter GLUT5. Portal systemic determined HPLC wild-type mice fed 7 days 0% free were <0.07 mM, independent of time after feeding, similar to those GLUT5(-/-), did not lead hyperglycemia. Postprandial levels, however, increased markedly isocaloric 20% fructose, causing significant...
Metabolic complications arising from excessive fructose consumption are increasing dramatically even in young children, but little is known about ontogenetic mechanisms regulating Glut5 [glucose transporter 5; encoded by the Slc2a5 (solute carrier family 2 member 5) gene]. expression low postnatally and does not increase, unless luminal systemic glucocorticoids present, until ≥ 14 days of age, suggesting substrate-inducible age- hormone-sensitive regulation. In present study, we perfused...
Current fructose consumption levels often overwhelm the intestinal capacity to absorb fructose. We investigated impact of malabsorption on endocrine function and addressed role microbiota in this process. To answer question, a mouse model moderate [ketohexokinase mutant (KHK)−/−] wild-type (WT) littermate mice were used received 20%-fructose (KHK-F WT-F) or 20%-glucose diet. Cholecystokinin (Cck) mRNA protein expression ileum cecum, as well preproglucagon (Gcg) neurotensin (Nts) increased...
A causal correlation between the metabolic disorders associated with sugar intake and disruption of gastrointestinal (GI) homeostasis has been suggested, but underlying mechanisms remain unclear. To unravel these mechanisms, we investigated effect physiological amounts fructose glucose on barrier functions inflammatory status in various regions GI tract cecal microbiota composition. C57BL/6 mice were fed chow diet given 15% or drinking water for 9 weeks. We monitored caloric intake, body...
Renal disease leads to perturbations in calcium and phosphate homeostasis vitamin D metabolism. Dietary fructose aggravates chronic kidney (CKD), but whether it also worsens CKD-induced derangements is unknown. Here, we fed rats diets containing 60% glucose or for 1 mo beginning 6 wk after 5/6 nephrectomy sham operation. Nephrectomized had markedly greater weight, blood urea nitrogen, serum levels of creatinine, phosphate, calcium-phosphate product; dietary significantly exacerbated all...
Overconsumption of fructose is linked to metabolic diseases, which are often associated with reduced fertility. GLUT5 the most specific transporter. To investigate its role in testes, we analyzed male reproductive phenotype transgenic mice deficient (GLUT5-/- or knockout [KO] mice). Glut5 expression was shown Leydig cells and germ cells, from primary spermatocytes spermatozoa. We found intratesticular pyruvate concentrations GLUT5-/- mice. These exhibited 30% lower litter sizes compared...
The changes in lipid composition of spermatozoa and seminal plasma motility, viability, morphological integrity were measured turkey semen diluted Beltsville poultry extender stored for 48 h (4°C). total phospholipid content decreased during storage, while no quantitative decrease was observed plasma. More precisely, significant decreases phosphatidylcholine, to a lesser extent sphingomyeline, phosphatidylserine, phosphatidylinositol spermatozoa. fatty acid profile partly reflected diet had...
We recently discovered that chronic high fructose intake by lactating rats prevented adaptive increases in rates of active intestinal Ca 2+ transport and levels 1,25-(OH) 2 D 3 , the form vitamin D. Since sufficient absorption is essential for skeletal growth, our discovery may explain findings excessive consumption sweeteners compromises bone integrity children. tested hypothesis mediates inhibitory effect on transport. First, compared with those fed glucose or starch, growing 4 wk had a...
Abstract Previous studies on germ‐free (GF) animals have described altered anxiety‐like and social behaviors together with dysregulations in brain serotonin (5‐HT) metabolism. Alterations circulating 5‐HT levels gut metabolism also been reported GF mice. In this study, we conducted an integrative analysis of various as well markers the along GI tract male mice compared conventional (CV) ones. We found a strong decrease locomotor activity, accompanied by some signs increased behavior CV Brain...
Background & Aims Excessive fructose intake is a growing public health concern, yet many individuals have lower absorption capacity than the average intake, leading to widespread chronic malabsorption. This results in intestinal spillover, disrupting gut microbiota and triggering peripheral inflammation, which, along with neuroinflammation, plays key role mood disorders. study investigates connection between malabsorption disorders by examining changes human cohort exploring their links...