A5011147929- Hippo pathway signaling and YAP/TAZ
- Developmental Biology and Gene Regulation
- Neurobiology and Insect Physiology Research
- Ubiquitin and proteasome pathways
- Invertebrate Immune Response Mechanisms
- Digital Holography and Microscopy
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Optical Coherence Tomography Applications
- Advanced Fluorescence Microscopy Techniques
- Protein Kinase Regulation and GTPase Signaling
- Advanced Proteomics Techniques and Applications
- Receptor Mechanisms and Signaling
- Insect Resistance and Genetics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genetic Syndromes and Imprinting
- RNA and protein synthesis mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Wnt/β-catenin signaling in development and cancer
- Plant Molecular Biology Research
- Cellular transport and secretion
- Down syndrome and intellectual disability research
- Advanced biosensing and bioanalysis techniques
- Genetics, Aging, and Longevity in Model Organisms
- Near-Field Optical Microscopy
University of Massachusetts Boston
2015-2024
Massachusetts General Hospital
2004-2005
Harvard University
2005
University of California, San Diego
1997-2002
Abstract We describe the application of tandem affinity purification–mass spectrometry (TAP‐MS) to study protein complexes in Drosophila . have constructed vectors for inducible expression TAP‐tagged fusion proteins cultured cells and vivo. Using these vectors, we tagged, as a paradigm, several components Notch signaling pathway, isolated containing baits associated from embryos, identified subunits by liquid chromatography–tandem mass (LC‐MS/MS). Several known interactions involving pathway...
AbstractTandem affinity purification (TAP) has been widely used for the analysis of protein complexes. We investigated parameters recently developed TAP method (GS-TAP) and its application in Drosophila. This new tag combination includes two Protein G modules a streptavidin binding peptide (SBP), separated by one or TEV protease cleavage sites. made pMK33-based GS-TAP vectors to allow generation stable cell lines using hygromycin selection inducible expression from metallothionein promoter,...
The putative promoter region of the SNRPN gene contains a CpG island which is heavily methylated in maternally derived allele and unmethylated paternally allele. In patients with Prader-Willi syndrome (PWS) only present, while those Angelman (AS) present. purpose this paper to report polymerase chain reaction (PCR)-based assay evaluate methylation status show that allows rapid diagnosis PWS AS. Methylated cytosines dinucleotide are resistant chemical modification by sodium bisulfite....
Significance The transcriptional repressor protein Capicua (Cic) is a conserved regulator of organ growth and tissue patterning, mutations in the CIC gene humans result brain cancer oligodendroglioma. Cic activity controlled by receptor tyrosine kinase (RTK) signaling pathway. Here, we identify Minibrain (Mnb) its adaptor Wings apart (Wap) as regulators. Mnb Wap bind to phosphorylate protein, inhibit ability repress expression. Mnb-dependent down-regulation necessary for proper multiple...
ABSTRACT We have characterized a protein isoform (CncB) from the Drosophila cap ‘n’ collar locus that selectively represses cis-regulatory elements are activated by Hox Deformed. Of three Cnc isoforms, CncB is expressed in localized pattern mandibular and labral cells of head during mid-stages embryogenesis. When absent or reduced, homeotically transformed toward maxillary identities. This transformation associated with persistent Deformed expression anterior cells, since autoactivation...
ABSTRACT The basic-leucine zipper protein Cap ‘n’ collar B (CncB) suppresses the segmental identity function of Hox gene Deformed (Dfd) in mandibular segment Drosophila embryos. CncB is also required for proper development intercalary, labral and structures. In this study, we provide evidence that CncB-mediated suppression Dfd requires homolog mammalian small Maf proteins, Maf-S, occurs even presence high amounts protein. Interestingly, CncB/Maf-S suppressive effect can be partially reversed...
A ligand-independent cleavage (S1) in the extracellular domain of mammalian Notch receptor results what is considered to be canonical heterodimeric form on cell surface. The vivo consequences and significance this Drosophila signaling remain unclear contradictory. We determined site examined its function by a transgenic analysis receptors that cannot cleaved. Our demonstrate correlation between loss receptor, supporting notion S1 an mechanism signal control.
The Toll signaling pathway has a highly conserved function in innate immunity and is regulated by multiple factors that fine tune its activity. One such factor β-arrestin Kurtz (Krz), which we previously implicated the inhibition of developmental Drosophila melanogaster embryo. Another level controlling activity immune system homeostasis protein sumoylation. In this study, have uncovered link between these two modes regulation show Krz affects sumoylation via interaction with SUMO protease,...
Healthy mitochondrial networks are maintained via balanced integration of quality control processes (biogenesis, fusion, fission, and mitophagy). The purpose this study was to investigate the effects severe obesity type 2 diabetes (T2D) on network morphology expression proteins regulating in cultured human myotubes. Primary skeletal muscle cells were isolated from biopsies lean, severely obese nondiabetic individuals diabetic (n = 8–9/group) differentiated Mitochondrial determined live...
Abstract Summary: The Drosophila protein DEAF‐1 is a sequence‐specific DNA binding that was isolated as putative cofactor of the Hox Deformed (Dfd). In this study, we analyze effects loss or gain function on development. Maternal/zygotic mutations largely result in early embryonic arrest prior to expression zygotic segmentation genes, although few embryos develop into larvae with defects variable severity. Overexpression can induce migration/closure dorsal epidermis, and overexpression adult...
Abstract Hox transcription factors, in combination with cofactors such as PBC proteins, provide diverse developmental fates to cells on the anteroposterior body axis of animal embryos. However, mechanisms by which different proteins and their generate those remain unclear. Recent findings have provided support for a model where DNA binding sites that directly interact Hox-PBC heterodimers determine member protein family occupies thereby regulates given target element. In experiments reported...
Lgl reduces Notch activation by cooperating with Vap33 to inhibit the vacuolar ATPase, thereby reducing endosome acidification.