A human gene encoding an interferon-induced 15-kDa protein has been isolated from a genomic library. The appears to be single-copy and is composed of two exons, the first which contains ATG translation initiation codon. In vitro nuclear run-on assays showed that transcription rate stimulated after interferon treatment. To analyze transcriptional regulatory sequences, we constructed recombinant plasmids for use in transient transfection HeLa cells. Constructs containing 115 nucleotides 5'...

Mouse glandular kallikreins are encoded by a family of closely linked genes which located on chromosome 7 at site corresponding to the genetically defined Tam-l, Prt-4, and Prt-6 loci.We have characterized 24 kallikrein genomic cloning restriction mapping 310 kilobase pairs BALBfc mouse DNA.Most these highly homologous, same exonfintron organization, in clusters up 11 genes.Partial sequence analysis has facilitated identification those members for protein data exist assignment pseudogenes or...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructure and chromosomal localization of the human renal kallikrein geneBronwyn A. Evans, Zhang Xiao Yun, Jacqueline Close, Geoffrey W. Tregear, Naomi Kitamura, Shigetada Nakanishi, David F. Callen, Elizabeth Baker, Valentine J. Hyland, Cite this: Biochemistry 1988, 27, 9, 3124–3129Publication Date (Print):May 3, 1988Publication History Published online1 May 2002Published inissue 3...

The reverse transcription/polymerase chain reaction was used to demonstrate β 3 ‐adrenoceptor mRNA in rat brain regions. Levels were highest hippocampus, cerebral cortex and striatum lower hypothalamus, brainstem cerebellum.

Glandular kallikreins are a highly homologous subfamily of serine proteases encoded by 25 genes in the mouse. Several gene products have been implicated as having specific roles growth factor biosynthesis, suggesting general role for members this family generation biologically active peptides (Mason, A.J., Evans, B.A., Cox, D.R., Shine, J., and Richards, R.I. (1983) Nature 303, 300-307). Here we describe identification, structure, expression renal kallikrein gene, major kinin-generating...

1. This study examines the expression of beta 3-adrenoceptor messenger RNA (beta 3-AR mRNA) in rat tissues to allow comparison with atypical beta-adrenoceptors determined by functional and radioligand binding techniques. 2. A reverse transcription/polymerase chain reaction protocol has been developed for determining relative amounts mRNA tissues. 3. Measurement adipsin uncoupling protein (UCP) was used examine all presence white brown adipose tissue which may contribute mRNA. 4. The is...

Although G protein-coupled receptors are often categorized in terms of their primary coupling to a given type Gα protein subunit, it is now well established that many show promiscuous and activate multiple signaling pathways. Furthermore, some agonists selectively pathways by promoting interaction between distinct receptor conformational states particular subunits or alternative proteins. We have tested the capacity stimulate Ca²⁺ release, cAMP accumulation, changes extracellular...

Introduction Ciliopathies are a group of human Mendelian disorders caused by dysfunction primary cilia, small quasi-ubiquitous sensory organelles. Patients suffering from ciliopathies often display prominent neurodevelopmental phenotypes, underscoring the importance cilia during development and for function central nervous system (CNS). Human tissues, in particular CNS, very hard to obtain research. Patient derived- or genetically engineered induced pluripotent stem cells (hiPSCs) therefore...

Inhibin is a glycoprotein hormone composed of two nonidentical subunits. It produced by the ovary and testis plays vital role in gonadal function inhibiting secretion FSH. More recently, additional activities associated with inhibin peptides have been identified. heterodimers (alpha-beta) are reported to act directly on ovarian granulosa cells inhibit estrogen production induced Furthermore, homodimers beta-inhibin subunits stimulate FSH, an activity that opposite inhibin. Each these...

β ‐adrenoceptor (AR) agonists increase 2‐deoxy‐[ 3 H]‐ D ‐glucose uptake (GU) via 2 ‐AR in rat L6 cells. The agonists, zinterol ( ‐AR) and (−)‐isoprenaline, increased cAMP accumulation a concentration‐dependent manner (pEC 50 =9.1±0.02 7.8±0.02). Cholera toxin (% max 141.8±2.5) the analogues, 8‐bromo‐cAMP (8Br‐cAMP) dibutyryl (dbcAMP), also GU (196.8±13.5 196.4±17.3%). adenylate cyclase inhibitor, 2′,5′‐dideoxyadenosine (50 μ M ), significantly reduced to (100 n ) (109.7+35.0 21.6+4.5 pmol...

This study examines signaling pathways activated by the mouse β₃-adrenoceptor (AR) expressed in Chinese hamster ovary cells at high (CHOβ₃H) or low (CHOβ₃L) levels. Functional responses included extracellular acidification rate (ECAR), cAMP accumulation, and p38 mitogen-activated protein kinase (MAPK) signal-regulated 1/2 (Erk1/2) phosphorylation. (–)-Isoproterenol β₃-AR agonist (<i>R</i>,...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMouse glandular kallikrein genes: identification and characterization of the genes encoding epidermal growth factor binding proteinsCatherine C. Drinkwater, Bronwyn A. Evans, Robert I. RichardsCite this: Biochemistry 1987, 26, 21, 6750–6756Publication Date (Print):October 1, 1987Publication History Published online1 May 2002Published inissue 1 October...

The receptor that mediates the increase in glucose transport (GT) response to β‐adrenoceptor (β‐AR) agonists was characterized rat skeletal muscle cell line L6, using 2‐deoxy‐[ 3 H]‐ D ‐glucose assay. β ‐AR agonist BRL37344 (pEC 50 =6.89±0.21), β‐AR isoprenaline =8.99±0.24) and 2 zinterol =9.74±0.15) increased GT as did insulin =6.93±0.15). highly selective CL316243 only weakly stimulated GT. pK B values calculated from shift of pEC presence 1 antagonist CGP 20712A or SR 59230A were not...

1. Functional and molecular approaches were used to characterize the beta-AR subtypes mediating relaxation of rat ileal smooth muscle. 2. In functional studies, (-)-isoprenaline was unchanged by CGP20712A (beta1-AR antagonist) or ICI118551 (beta2-AR but shifted propranolol (pKB=6.69). (+/-)-Cyanopindolol, CGP12177 ICID7114 did not cause antagonized relaxation. 3. BRL37344 (beta3-AR agonist) caused biphasic The high affinity component with low propranolol, (+/-)-cyanopindolol, tertatolol...

The β 3 ‐adrenoceptor (AR) differs from the 1 ‐AR and 2 ‐ARs in having introns within downstream of coding block. This study demonstrates two splice variants mouse which differ region. Reverse transcription/polymerase chain reaction with intron‐spanning primers was used to demonstrate variant ‐adrenoceptor. novel 3b has 17 amino acids encoded by exon (SSLLREPRHLYTCLGYP) 13 known 3a (RFDGYEGARPFPT). mRNA is differentially expressed tissues, levels relative highest hypothalamus, cortex white...

Background and Purpose Insulin‐like peptide 5 (INSL5) is a two‐chain, three‐disulfide‐bonded of the insulin/relaxin superfamily, uniquely expressed in enteroendocrine L ‐cells colon. It cognate ligand relaxin family RXFP4 receptor that mainly colorectum enteric nervous system. This study identifies new signalling pathways activated by INSL5 acting on receptors. Experimental Approach INSL5/RXFP4 was investigated using AlphaScreen® proximity assays. Recruitment Gα i/o proteins receptors...