A5029080160- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Fungal and yeast genetics research
- Plant Molecular Biology Research
- RNA modifications and cancer
- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Genetics and Neurodevelopmental Disorders
University of Geneva
2014-2025
University of Genoa
2012
In yeast, ribosome production is controlled transcriptionally by tight coregulation of the 138 ribosomal protein genes (RPGs). RPG promoters display limited sequence homology, and molecular basis for their remains largely unknown. Here we identify two prevalent promoter types, both characterized upstream binding general transcription factor (TF) Rap1 followed RPG-specific Fhl1/Ifh1 pair, with one type also HMG-B Hmo1. We show that regulatory properties types are remarkably similar,...
The transcriptional coactivators Mediator and two histone acetyltransferase (HAT) complexes, NuA4 SAGA, play global roles in activation. Here we explore the relative contributions of these factors to RNA polymerase II association at specific genes gene classes by rapid nuclear depletion key complex subunits. We show that HAT Esa1 differentially affects certain groups genes, whereas SAGA Gcn5 has a weaker but more uniform effect. Relative dependence on Tra1, shared component distinguishes...
Acetylation of histone H3 at residue K9 (H3K9ac) is a dynamically regulated mark associated with transcriptionally active promoters in eukaryotes. However, our understanding the relationship between H3K9ac and gene expression remains mostly correlative. In this study, we identify large suite growth-related (GR) genes yeast that undergo particularly strong down-regulation both transcription promoter-associated upon stress, delineate roles transcriptional activators (TAs), repressors, SAGA...
Abstract Mittal et al. (2021; first brought to our attention in May 2019) have raised concerns regarding the Chromatin Endogenous Cleavage-sequencing (ChEC-seq) technique (Zentner al., 2015) that may create a false impression this method has fundamental flaws which prevent one from distinguishing between signal and noise. Although focus on studies of global co-activators SAGA, TFIID Mediator we were not involved in, feel obliged highlight here several own publications (Albert 2019; Bruzzone...
Abstract Precise nucleosome organization at eukaryotic promoters is thought to be generated by multiple chromatin remodeler (CR) enzymes and affect transcription initiation. Using an integrated analysis of binding displacement activity following rapid depletion, we investigate the interplay between these their impact on in budding yeast. We show that many are acted upon CRs operate either cooperatively or opposition position key start site-associated +1 nucleosome. Functional assays suggest...
Summary Accessible chromatin is important for RNA polymerase II recruitment and transcription initiation at eukaryotic promoters. We investigated the mechanistic links between promoter DNA sequence, nucleosome positioning transcription. Our results indicate that precise of start site-associated +1 in yeast critical efficient TBP binding, driven by two key factors, essential remodeler RSC a small set ubiquitous pioneer factors. find no evidence but show instead strength directionality action...
A series of recent studies demonstrated an unexpectedly high frequency intronic RNA polymerase (pol) III transcription units spread throughout the human genome. The investigation a subset these transcripts revealed their tissue/cell-specific together with involvement in relevant physiopathological pathways. Despite this evidence, did not seem to have murine orthologs, based on nucleotide sequence, resulting limitation experimental approaches aimed study function. In work, we extended our...