Abstract In the absence of telomerase, telomeres progressively shorten with every round DNA replication, leading to replicative senescence. telomerase-deficient Saccharomyces cerevisiae, shortest telomere triggers onset senescence by activating damage checkpoint and recruiting homologous recombination (HR) factors. Yet, molecular structures that trigger this mechanisms repair have remained elusive. By tracking individual telomeres, we show are subjected different pathways depending on their...

Maturation of the 40S ribosomal subunit precursors in mammals mobilizes several non-ribosomal proteins, including atypical protein kinase RioK2. Here, we have investigated involvement another member RIO family, RioK3, human ribosome biogenesis. RioK3 is a cytoplasmic that does not seem to shuttle between nucleus and cytoplasm via Crm1-dependent mechanism as RioK2 which sediments with particles sucrose gradient. When small biogenesis impaired by depletion either rpS15, rpS19 or RioK2,...

Eukaryotic genomes are almost entirely transcribed by RNA polymerase II. Consequently, the transcription of long noncoding RNAs often overlaps with coding gene promoters, triggering potential repression through a poorly characterized mechanism interference. Here, we propose comprehensive model chromatin-based interference in Saccharomyces cerevisiae (S. cerevisiae). By using transcription-inducible strain, analyze relationship between antisense elongation and sense repression, nucleosome...

Acetylation of histone H3 at residue K9 (H3K9ac) is a dynamically regulated mark associated with transcriptionally active promoters in eukaryotes. However, our understanding the relationship between H3K9ac and gene expression remains mostly correlative. In this study, we identify large suite growth-related (GR) genes yeast that undergo particularly strong down-regulation both transcription promoter-associated upon stress, delineate roles transcriptional activators (TAs), repressors, SAGA...

In eukaryotic organisms, replication initiation follows a temporal program. Among the parameters that regulate this program in Saccharomyces cerevisiae , chromatin structure has been at center of attention without considering contribution transcription. Here, we revisit light widespread genomic noncoding We find RNA transcription termination vicinity autonomously replicating sequences (ARSs) shields from transcriptional readthrough. Consistently, high natural nascent correlates with low ARS...

Pervasive transcription of eukaryotic genomes generates non-coding transcripts with regulatory potential. We examined the effects antisense on regulation expression yeast PHO5 gene, a paradigmatic case for gene through promoter chromatin remodeling. A negative role at locus was demonstrated by leveraging level overlapping specific mutant backgrounds, from strong in cis , and use CRISPRi system. Furthermore, we showed that enhanced elongation leads to more repressive conformation promoter,...

DNA-protein crosslinks (DPCs) challenge faithful DNA replication and smooth passage of genomic information. Our study unveils the cullin E3 ubiquitin ligase Rtt101 as a DPC repair factor. Genetic analyses demonstrate that is essential for resistance to wide range types including topoisomerase 1 crosslinks, in same pathway ubiquitin-dependent aspartic protease Ddi1. Using an vivo inducible Top1-mimicking system, we reveal significant impact ubiquitination on removal across different cell...

Histone turnover during gene transcription is mediated by regulatory proteins, not the machinery.

Eukaryotic genomes are pervasively transcribed by RNA polymerase II (RNAPII), and transcription of long non-coding RNAs often overlaps with coding gene promoters. This might lead to repression in a process named Transcription Interference (TI). In Saccharomyces cerevisiae, TI is mainly driven antisense occurs through re-shaping promoter Nucleosome-Depleted Regions (NDRs). this study, we developed genetic screen identify new players involved Antisense-Mediated (AMTI). Among the candidates,...

ABSTRACT Replication disrupts chromatin organization. Thus, the rapid resetting of nucleosome positioning is essential to maintain faithful gene expression. The initial step this reconfiguration occurs at Nucleosome-Depleted Regions (NDRs). While studies have elucidated role Transcription Factors (TFs) and Chromatin Remodelers (CRs) in vitro or maintaining NDRs vivo , none has addressed their function shortly after replication. Through purification nascent yeast, we dissected choreography...

Abstract Eukaryotic genomes are almost entirely transcribed by RNA polymerase II (RNAPII). Consequently, the transcription of long noncoding RNAs (lncRNAs) often overlaps with coding gene promoters triggering potential repression through a poorly characterized mechanism interference. In this study, we propose global model chromatin-based interference in Saccharomyces cerevisiae ( S. ). By using inducible strain, analyzed relationship between antisense elongation and sense repression,...

ABSTRACT In Eukaryotic organisms, replication initiation follows a temporal program. Among the parameters that regulate this program in Saccharomyces cerevisiae , chromatin structure has been at center of attention without considering contribution transcription. Here, we revisit light pervasive We find noncoding RNA transcription termination vicinity origins or ARS (Autonomously Replicating Sequences) maximizes by restricting transcriptional readthrough into ARS. Consistently, high natural...

Abstract Pervasive transcription of eukaryotic genomes generates non-coding transcripts with regulatory potential. We examined the effects antisense on regulation expression yeast PHO5 gene, a paradigmatic case for gene through promoter chromatin remodeling. A negative role at locus was demonstrated by leveraging level overlapping specific mutant backgrounds, from strong in cis , and use CRISPRi system. Furthermore, we showed that enhanced elongation leads to more repressive conformation...

Abstract Eukaryotic genomes are pervasively transcribed by RNA polymerase II (RNAPII), and transcription of long non-coding RNAs often overlaps with coding gene promoters. This might lead to repression in a process named Transcription Interference (TI). In Saccharomyces cerevisiae ( S. ), TI is mainly driven antisense occurs through re-shaping promoter Nucleosome-Depleted Regions (NDRs). this study, we developed genetic screen identify new players involved Antisense-Mediated (AMTI). Among...