A5008128647- Microbial Metabolic Engineering and Bioproduction
- Photosynthetic Processes and Mechanisms
- Bacterial Genetics and Biotechnology
- Pregnancy and preeclampsia studies
- Digital Mental Health Interventions
- Ocular Disorders and Treatments
- Patient Satisfaction in Healthcare
- Diabetic Foot Ulcer Assessment and Management
- Wound Healing and Treatments
- Preterm Birth and Chorioamnionitis
- Astrophysical Phenomena and Observations
- Impact of Technology on Adolescents
- Geriatric Care and Nursing Homes
- Pressure Ulcer Prevention and Management
- Patient-Provider Communication in Healthcare
- Prenatal Screening and Diagnostics
- Palliative Care and End-of-Life Issues
- Primary Care and Health Outcomes
- Pregnancy-related medical research
- Astro and Planetary Science
- Pelvic floor disorders treatments
- Mental Health and Patient Involvement
- Anxiety, Depression, Psychometrics, Treatment, Cognitive Processes
- Healthcare innovation and challenges
- Data Analysis and Archiving
University of Oxford
2007-2024
Health Affairs
2024
University of Kansas
2020-2023
NHS Fife
2019-2021
Victoria Hospital
2020-2021
The University of Kansas Health System
2021
Royal North Shore Hospital
2021
Nuffield Health
2020
Queen Margaret Hospital
2019-2020
University of Tennessee at Knoxville
2019
<h3>Background:</h3> Developmental eye anomalies, which include anophthalmia (absent eye) or microphthalmia (small are an important cause of severe visual impairment in infants and young children. Heterozygous mutations SOX2, a SOX1B-HMG box transcription factor, have been found up to 10% individuals with such could also be associated range non-ocular abnormalities. <h3>Methods:</h3> We performed mutation analysis on new cohort 120 patients congenital abnormalities, mainly anophthalmia,...
FOXE3 is a lens-specific transcription factor with highly conserved forkhead domain previously implicated in congenital primary aphakia and anterior segment dysgenesis. Here, we identify new recessive mutations causative for microphthalmia, sclerocornea, aphakia, glaucoma two extended consanguineous families by SNP array genotyping followed candidate gene approach. Following an additional screen of 236 subjects developmental eye anomalies, report further novel heterozygous segregating...
Hospice provides an opportunity for patients to receive care at home the end of life (EOL); however, approximately 25% who disenroll from hospice are hospitalized. Hospitalization can lead poor transitions and result in unwarranted adverse patient outcomes. Research examining reasons hospitalization this population is limited.The objective was understand among through perspectives interdisciplinary team (IDT) members.This a qualitative study using grounded theory approach. Seven...
Background Many people are accessing digital self-help for mental health problems, often with little evidence of effectiveness. Social anxiety is one the most common sources distress in population, and many symptoms do not seek help what represents a significant public problem. Objective This study aimed to evaluate effectiveness self-guided cognitive behavioral internet intervention social general population. Methods We conducted two-group randomized controlled trial England between May 11,...
Summary Components of bacterial chemosensory pathways which sense via transmembrane receptors have been shown to localize the cell poles. Many species, however, operons encoding multiple putative pathways, some including cytoplasmic receptors. In‐genome fusions single or genes components two in Rhodobacter sphaeroides , che Op 2 and 3 revealed that while sensory transducing proteins associated with encoded on were targeted poles, all a cluster. No localized both sites. These data show...
Summary The purple photosynthetic bacterium Rhodobacter sphaeroides has three loci encoding multiple homologues of the bacterial chemosensory proteins: 13 putative chemoreceptors, four CheW, CheA, six CheY, two CheB and CheR. Previously, studies have shown that, although deletion che Op 1 led to only minor changes in behaviour, 2 a loss taxis. third locus encodes one CheR, CheB, cytoplasmic chemoreceptor (TlpT) protein showing homology chromosomal partitioning factor Soj (designated Slp)....
Background Traditional health information has been based on facts and figures not patient experience (PEx). Websites featuring people’s accounts of their experiences illness are popular as a source information, support much else. However, there concerns that experiential the internet might have adverse effects health. Aims To find out whether, when how NHS should incorporate PEx into online elucidate mechanisms through which influence health, develop tool to measure PEx, explore is used,...
Objective To determine the feasibility of a randomised controlled trial (RCT) assessing effects an experience-based website as resource for self-management chronic asthma. Design and setting Feasibility, single-blind RCT in 2 regions England. Randomisation used computer-generated random number sequence 1:1 ratio, after baseline data collection, to access weeks. Participants Adults (age ≥18 years), with clinically diagnosed asthma coded their primary care electronic record, prescribed inhaled...
Objectives Improving patient experience is widely regarded as a key component of health care quality. However, while considerable amount data are collected about experience, there concerns this information not always used to improve care. This study explored whether and how frontline staff use for service improvement. Methods We conducted year-long ethnographic case evaluation, including 299 hours observations 95 interviews, in six medical wards at different hospital sites the United Kingdom...
Rhodobacter sphaeroides has multiple homologues of most the Escherichia coli chemotaxis genes, organized in two major operons and other, unlinked, loci. These include cheA 1 cheW ( che Op ) 2 , 3 ). We have deleted each these in‐frame examined chemosensory behaviour strains on swarm plates tethered cell assays. In addition, we effect deletions polar localization chemoreceptor McpG. E. deletion either or results a non‐chemotactic phenotype, also show no receptor clustering. Here, demonstrate...
Summary TlpC is encoded in the second chemotaxis operon of Rhodobacter sphaeroides . This protein shows some homology to membrane‐spanning chemoreceptors many bacterial species but, unlike these, essential for R. all compounds tested. Genomic replacement tlpC with a C‐terminal gfp fusion demonstrated that localized discrete cluster within cytoplasm. Immunogold electron microscopy also showed cytoplasmic electron‐dense region. Correct TlpC–GFP localization depended on downstream signalling...
Genes coding for a classical membrane spanning chemoreceptor ( mcpG ) and response regulator cheY4 were identified in region of Rhodobacter sphaeroides DNA unlinked to either the two previously chemosensory operons. Immunogold electron microscopy had shown that expression chemoreceptors R. varies with growth conditions. Using GFP fused newly McpG, we examined targeting this single methyl‐accepting chemotaxis protein (MCP) under different The gene encoding C‐terminal McpG–GFP fusion was...
The Escherichia coli two-component chemosensory pathway has been extensively studied, and its response regulator, CheY, become a paradigm for regulators. However, unlike E. coli, most chemotactic nonenteric bacteria have multiple CheY homologues. roles cellular localization of the CheYs in Rhodobacter sphaeroides were determined. Only two required chemotaxis, CheY6 either CheY3 or CheY4. These partially localized to chemotaxis signaling clusters, with remaining protein delocalized....
Approximately 25% of hospice disenrollments in the United States occur as result hospitalization, which can lead to burdensome transitions and undesired care. Informal caregivers (e.g., spouses, children) play a critical role caring for patients on home hospice. Research examining hospital-related disenrollment among these is limited.To understand events surrounding hospitalization discharged from through perspective their informal caregivers.Thirty-eight semistructured phone interviews with...
Background Online customer feedback has become routine in many industries, but it yet to be harnessed for service improvement health care. Objectives To identify the current evidence on online patient feedback; public and professional attitudes behaviour relation explore experiences of patients providing NHS; examine practices processes within NHS trusts. Design A multimethod programme five studies: (1) synthesis stakeholder consultation; (2) questionnaire survey public; (3) qualitative...
Background and aim The NHS collects a large number of data on patient experience, but there are concerns that it does not use this information to improve care. This study explored whether or how front-line staff experience for service improvement. Methods Phase 1 – secondary analysis existing national survey data, new trust leads. 2 case studies in six medical wards using ethnographic observations interviews. A baseline follow-up were conducted each ward, supplemented by in-depth Following...
People with learning disabilities are living longer. Despite government policy to encourage people lead supported lives in their community, family carers often maintain support due dissatisfaction services. This can moving from the home a crisis.
ABSTRACT Rhodobacter sphaeroides has multiple homologues of most the Escherichia coli chemotaxis genes, organized in three major operons and other, unlinked, loci. These include cheA 1 cheR ( che Op ) 2 , cheB ). In-frame deletions these were constructed chemosensory behaviour resultant mutants examined on swarm plates tethered cell assays. Under conditions tested, CheR CheB essential for normal chemotaxis, whereas was not. but not also able to complement equivalent E. mutants. However, none...
Many proteins have recently been shown to localize different regions of the bacterial cell. This is most striking in case Escherichia coli chemotaxis pathway which components at cell poles. Rhodobacter sphaeroides has a more complex system with two complete pathways, each localizing positions, one pole and discrete cluster within cytoplasm bacterium. Using genomic replacement wild-type genes R. their corresponding fluorescent protein fusions conjunction frame deletions other genes, we...