A5009018422- Salmonella and Campylobacter epidemiology
- Food Safety and Hygiene
- Listeria monocytogenes in Food Safety
- Abdominal Surgery and Complications
- Identification and Quantification in Food
- Meat and Animal Product Quality
- Animal Disease Management and Epidemiology
- Veterinary medicine and infectious diseases
- Phytochemicals and Antioxidant Activities
- Clinical Nutrition and Gastroenterology
- Food Supply Chain Traceability
- Respiratory Support and Mechanisms
- Essential Oils and Antimicrobial Activity
- Animal Nutrition and Physiology
- Hernia repair and management
- Intestinal and Peritoneal Adhesions
- Food Waste Reduction and Sustainability
- Organic Food and Agriculture
- Antimicrobial Resistance in Staphylococcus
- Escherichia coli research studies
- Congenital Diaphragmatic Hernia Studies
- Spectroscopy and Chemometric Analyses
- Toxoplasma gondii Research Studies
- Adipose Tissue and Metabolism
- Molecular Biology Techniques and Applications
Aristotle University of Thessaloniki
2013-2024
University College Dublin
2012
University of Ulster
2012
AHEPA University Hospital
2011
Over the recent years, next generation sequencing and microarray technologies have revolutionized scientific research with their applications to high-throughput analysis of biological systems. Isolation high quantities pure, intact, double stranded, highly concentrated, not contaminated genomic DNA is prerequisite for successful reliable large scale genotyping analysis. High pure are also required creation DNA-banks. In present study, eleven different extraction procedures, including...
In the last decades, a risk-based approach has been identified as step forward in modernising meat safety system Europe. Risk categorisation of abattoirs based on their process hygiene and appropriate use harmonised epidemiological indicators (HEIs) suggested one essential component assurance system. However, to date, only limited number papers have investigated abattoir risk categorisation. Therefore, objectives this study were (i) provide an overview systems poultry, pig, cattle small...
The aim of the present study was to address method-dependent implications during quantification viable Campylobacter coli cells on meat over time. Traditional colony counting selective and non-selective culture media along with an optimized viability real-time PCR utilizing propidium monoazide-quantitative (PMA-qPCR), spheroplast formation internal sample process control (ISPC), were comparatively evaluated for monitoring survival C. fresh lamb refrigeration storage under normal atmospheric...
The presence, genetic diversity, and antimicrobial susceptibility profile of Campylobacter spp. in retail lamb goat kid carcasses were assessed. A total 200 samples consisting 100 meat liver surface swabs collected from 47 53 at 23 markets Northern Greece, 125 isolates recovered 32 surfaces (32%) 44 (44%). Multiplex polymerase chain reaction restriction fragment length polymorphism analysis specified coli as the most frequently detected species (59.2%) followed by C. jejuni (40.8%)....
Methicillin-resistant Staphylococcus aureus (MRSA) presents major health risk for humans causing serious nosocomial and community-acquired infections. Asymptomatic food-producing animal carriers their meat may represent potential reservoirs human The aim of this study was to investigate the prevalence MRSA in small ruminants raised under free-range conditions at slaughter retail level Northern Greece. isolated from 9·6% examined samples. All isolates were resistant least one antibiotic,...
Proper training of official veterinarians (OVs) is fundamental to achieving their advanced role as risk managers within a risk-based meat safety assurance system (RB-MSAS) addressing the most relevant meat-borne public health hazards. The demography, opportunities and needs OVs in Europe remain largely unknown. objective this study was characterise terms demography employment identify continuing education via survey. An anonymous questionnaire with 32 questions designed, translated into 17...
In most European countries, findings detected at post-mortem inspection (PMI) of broilers slaughter are registered using national or regional code systems. The aim this study was to map and compare existing broiler PMI systems in Europe suggest a new harmonized risk-based set intended be interpreted the same way all countries. Information about systems, condemnation criteria, proportion unfit carcasses 2022, as well reasons justifying condemnation, were collected from nine countries through...
The replacement of soybean meal (SBM) from intensively reared dairy sheep diets has emerged as a significant challenge for sustainable production. However, the effects this on milk production have not been sufficiently elucidated. objective study was to prospectively assess replacing SBM with mixture alternative protein sources yield (MY) and quality traits (MQT) in sheep. A total 112 multiparous, purebred milking ewes Chios Frizarta breeds, two intensive farms, were involved study,...
A viability quantitative PCR (qPCR) utilizing propidium monoazide (PMA) is presented for rapid quantification of viable cells using the foodborne pathogen Campylobacter coli as a bacterial model. It includes optimized spheroplast formation via lysozyme and EDTA, induction mild osmotic shock enhancing selective penetration PMA into dead cells, exploitation an internal sample process control (ISPC) involving cell inactivation to assess residual false-positive signals within each sample....
The objective of the present study was to investigate effect whey on broiler chicks' performance, welfare, and caecal Campylobacter counts under experimental field conditions. In study, 120-d-old chicks were randomly allocated four treatment groups, as described below: group A, which served negative control; B, fed with a diet supplemented 1% whey; C, challenged jejuni; D, C. jejuni, respectively. Performance indexes jejuni calculated. addition performance indexes, hock burn foot pad...
Aim: In the current study, a contribution to knowledge on prevalence and level of contamination Listeria monocytogenes in ready-to-eat (RTE) seafood marketed Thessaloniki (Northern Greece) was provided; serovar identity L. isolates also determined. Materials Methods:A total 132 RTE samples consisting 74 smoked fish products, 18 salted 16 dried 9 raw marinated 10 cooked cephalopods 5 surimi crab stick products were analyzed.L. isolated enumerated based ISO 11290-1/A1 11290-2/A1 protocols,...
The study was conducted to determine the prevalence, antimicrobial resistance and risk factors for Campylobacter colonising dogs cats in Greece.Faecal specimens were collected from 181 132 cats.Culture methods applied detect spp.and a multiplex PCR assay identify isolates.The prevalence of spp. 3.8% 12.1% cats.The most frequently identified species C. jejuni (57.1%) followed by coli (42.9%).All feline isolates as apart one isolate that characterised Campylobacter-like organism.Gender, age,...
Formulations based on vegetable or fish oil and modifications in the production technology of dry fermented sausages have emerged recent years aiming to achieve desirable target reducing fat content these meat products. However, previous efforts confronted many difficulties, such as high mass loss unacceptable appearance due intensely wrinkled surfaces case hardening. The objective this study is produce evaluate by utilising a protein-olive emulsion substitute indigenous lactic acid bacteria...