A5086246342- Cardiac tumors and thrombi
- Peptidase Inhibition and Analysis
- Virus-based gene therapy research
- Cancer-related gene regulation
- Phosphodiesterase function and regulation
- Growth Hormone and Insulin-like Growth Factors
- Animal Disease Management and Epidemiology
- Herpesvirus Infections and Treatments
- Animal Virus Infections Studies
- Sarcoma Diagnosis and Treatment
- Animal Behavior and Welfare Studies
- Viral gastroenteritis research and epidemiology
- Autophagy in Disease and Therapy
- Chronic Myeloid Leukemia Treatments
- Genetic and phenotypic traits in livestock
- Meat and Animal Product Quality
- Milk Quality and Mastitis in Dairy Cows
- Renin-Angiotensin System Studies
- Protein Degradation and Inhibitors
- NF-κB Signaling Pathways
- Effects of Environmental Stressors on Livestock
- Viral Infectious Diseases and Gene Expression in Insects
- Immunotherapy and Immune Responses
- Reproductive Physiology in Livestock
- Hormonal Regulation and Hypertension
Aristotle University of Thessaloniki
2020-2024
Health and Human Development (2HD) Research Network
2004-2023
Agricultural University of Athens
2016-2020
University of Maryland, College Park
2003-2014
Centre for Research and Technology Hellas
2014
Virginia–Maryland College of Veterinary Medicine
2009-2011
Johns Hopkins University
2007-2010
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2004-2010
National Institutes of Health
2003-2008
Pediatrics and Genetics
2008
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, on this topic has continued to accelerate, and many new scientists have entered field. Our knowledge base relevant technologies also been expanding. Accordingly, it is important update these monitoring autophagy different organisms. Various reviews described range assays that used purpose. Nevertheless, there continues be confusion regarding acceptable methods measure autophagy, especially...
We generated a monoclonal antibody, RG-1, that binds to highly conserved L2 residues 17 36 and neutralizes human papillomavirus 16 (HPV16) HPV18. Passive immunotherapy with RG-1 was protective in mice. Antiserum the HPV16 peptide comprising (peptide 17-36) neutralized pseudoviruses HPV5, HPV6, HPV16, HPV 18, HPV31, 45, 52, 58, bovine 1, HPV11 native virions. Depletion of 17-36-reactive antibodies from cross-neutralizing rabbit L2-specific sera abolished cross-neutralization drastically...
Prenatal influenza exposure increases the risk for schizophrenia and brings to question how other respiratory viruses may contribute neuropsychiatric disease etiopathology. Human coronaviruses cause infections that range in seriousness from common colds severe acute syndrome. Like influenza, can be neurotropic. To test associations between serious mental disorders, we utilized a recently developed assay measured immunoglobulin G (IgG) response against 4 human coronavirus strains (229E, HKU1,...
Angus × Hereford heifers were used to determine endocrine and ovarian function preceding nutritionally induced anovulation. Six fed maintain body condition score (M), 12 a restricted diet (R) until they became anovulatory. Starting on d 13 of an estrous cycle, given PGF2α every 16 thereafter synchronize cycles. Ovarian structures M R monitored by ultrasonography daily from 8 ovulation (d 1 the subsequent cycle) Concentrations LH FSH quantified in serum samples collected 10 min for h 2 15 (48...
Coronaviruses cause respiratory infections ranging from common colds to severe acute syndrome (SARS) in humans. Estimates for exposure non-SARS coronaviruses are high, particularly 229E and OC43; however, less information regarding seroprevalence is available HKU1 NL63. To measure rates these four coronavirus strains (229E, HKU1, NL63, OC43), we devised an immunoassay based on amino- carboxy-terminally tagged recombinant nucleocapsid antigens. Four human one feline antigen were cloned into...
Hepatitis E virus (HEV) is the causative agent of hepatitis E, a major form viral in developing countries. The open reading frame 3 (ORF3) HEV encodes phosphoprotein with molecular mass approximately 13 kDa (hereinafter called vp13). vp13 essential for establishing infections animals, yet its exact functions are still obscure. Our current study found evidence showing interaction between and microtubules. Live-cell confocal fluorescence microscopy revealed both filamentous punctate...
Abstract Purpose: Since the identification of PRKAR1A mutations in Carney complex, substitutions and small insertions/deletions have been found ∼70% patients. To date, no germ-line deletion and/or insertion exceeded a few base pairs (up to 15). Although families map chromosome 2, it is possible that current sequencing techniques do not detect larger gene changes PRKAR1A–mutation-negative individuals with complex. Experimental Design: screen for gross alterations gene, we applied Southern...
Abstract Several types of adrenocortical tumors that lead to Cushing syndrome may be caused by aberrant cyclic AMP (cAMP) signaling. We recently identified patients with micronodular hyperplasia who were carriers inactivating mutations in the 2q-located phosphodiesterase 11A (PDE11A) gene. now studied frequency two missense substitutions, R804H and R867G, conserved regions enzyme several sets normal controls, including 745 individuals enrolled a longitudinal cohort study, New York Cancer...
The human PRKAR1A gene encodes the regulatory subunit 1-alpha (RIalpha) of cAMP-dependent protein kinase A (PKA) holoenzyme. Regulation catalytic activity PKA is only well-studied function RIalpha. Inactivating mutations cause primary pigmented nodular adrenocortical disease (PPNAD) or Carney complex (CNC), an inherited syndrome associated with abnormal skin pigmentation and multiple neoplasias, including PPNAD. Histochemistry tissues from CNC patients indicative autophagic deficiency this...
The use of critical-for-life organs (e.g., liver or lung) for systemic gene therapeutics can lead to serious safety concerns. To circumvent such issues, we have considered salivary glands (SGs) as an alternative target tissue. Given the high secretory abilities SGs, hypothesized that administration low doses recombinant adeno-associated virus (AAV) vectors would allow therapeutic levels transgene-encoded proteins in bloodstream. We administered 10 9 particles AAV vector encoding human...
Nutritionally induced anovulatory and cyclic Angus x Hereford heifers were used to evaluate follicular growth concentrations of hormones metabolites during anovulation resumption ovulation. Anovulatory fed gain 0.6 (LGAIN) or 1.5 (HGAIN) kg/day until ovulation, with normal estrous cycles a maintenance diet (M). Follicles >/= 4 mm in diameter measured by daily ultrasonography HGAIN LGAIN one wave before realimentation (Wan) two waves (W-2, W-1) immediately the resulting first ovulation...
Pituitary morphology in patients with Prop1 gene mutations varies. Most demonstrate a normal or small pituitary gland. Occasionally, enlargement of undetermined origin has also been detected. In the present study we use long-term magnetic resonance imaging findings to characterize morphological abnormalities gland 15 (aged 2.5-45 yr) combined hormone deficiency caused by (GA296del/GA296del seven, GA296del/A150del two, A150del/A150del five, and GA296del/R73H one patient) attempt uncover...
Recent studies have proposed that adeno-associated viruses (AAVs) are not evolutionarily linked to other mammalian autonomous parvoviruses but more closely the of birds. To better understand relationship between primate and avian AAVs (AAAVs), we cloned sequenced genome an AAAV (ATCC VR-865) generated recombinant particles. The is 4,694 nucleotides in length has organization similar AAVs. entire displays 56 65% identity at nucleotide level with known inverted terminal repeats 142...
ABSTRACT To better understand the relationship between primate adeno-associated viruses (AAVs) and those of other mammals, we have cloned sequenced genome an AAV found as a contaminant in two isolates bovine adenovirus that was reported to be serologically distinct from AAVs. The (BAAV) has 4,693 bp, its organization is similar isolates. left-hand open reading frame (ORF) both inverted terminal repeats (ITRs) highest homology with rep ORF ITRs serotype 5 (AAV-5) (89 96%, respectively)....
Salivary glands (SGs) exhibit several important features which are also common to endocrine glands: self-containment due a surrounding capsule, highly efficient protein production and the ability secrete proteins into bloodstream. We have hypothesized that SGs potentially useful as gene transfer targets for correction of inherited monogenetic disorders. In present communication, we extend our studies attempt test hypothesis by comparing efficacy two commonly used viral vectors resulting...
Primary pigmented nodular adrenocortical disease, associated with Carney complex, is caused by mutations in PRKAR1A (mt-PRKAR1A), a gene that codes for the regulatory subunit type 1alpha (RIalpha) of cAMP-dependent protein kinase (PKA). inactivation dysregulated PKA activity thought to result tumorigenesis. mt-PRKAR1A-bearing lymphocytes from complex patients exhibit enhanced cell proliferation increased expression MAPK ERK1/2 pathway.The objective study was determine how and its subunits...