A5059265805- Pancreatic function and diabetes
- Phagocytosis and Immune Regulation
- Molecular Biology Techniques and Applications
- Neuropeptides and Animal Physiology
- Renin-Angiotensin System Studies
- CRISPR and Genetic Engineering
- Phosphodiesterase function and regulation
- Diabetes and associated disorders
- Cancer Genomics and Diagnostics
- Prostate Cancer Treatment and Research
- Sulfur Compounds in Biology
- Hormonal and reproductive studies
- Immune cells in cancer
- Peptidase Inhibition and Analysis
- Xenotransplantation and immune response
- Receptor Mechanisms and Signaling
- Advanced biosensing and bioanalysis techniques
- Estrogen and related hormone effects
- Renal Transplantation Outcomes and Treatments
- Nitric Oxide and Endothelin Effects
- Lipid metabolism and biosynthesis
- Complement system in diseases
- Cardiac Ischemia and Reperfusion
- Melanoma and MAPK Pathways
- MicroRNA in disease regulation
Material Measurement Laboratory
2017-2019
National Institute of Standards and Technology
2017-2019
National Institutes of Health
2006-2016
National Cancer Institute
2009-2016
Center for Cancer Research
2011-2016
George Washington University
2010-2016
National Institute of Standards
2016
Charles R. Drew University of Medicine and Science
2015
Cancer Institute (WIA)
2013
Cancer Genetics (United States)
2011
Every organ in the body requires blood vessels for efficient delivery of oxygen and nutrients, but independent vascular beds are highly specialized to meet individual needs specific organs. The vasculature brain is tightly sealed, with blood–brain barrier (BBB) properties developing coincident neural vascularization. G protein-coupled receptor 124 (GPR124) (tumor endothelial marker 5, TEM5), an orphan member adhesion family receptors, was previously identified on basis its overexpression...
Interactions between membrane proteins and the soluble fraction are essential for signal transduction regulating nutrient transport. To gain insights into membrane-based interactome, 3,852 open reading frames (ORFs) out of a target list 8,383 representing signaling from Arabidopsis thaliana were cloned Gateway compatible vector. The mating-based split-ubiquitin system was used to screen potential protein-protein interactions (pPPIs) among 490 ORFs. A binary robotic 142 receptor-like kinases,...
H(2)S is an endogenous signaling molecule that may act via protein sulfhydrylation to regulate various physiological functions. also a byproduct of dietary sulfate metabolism by gut bacteria. Inflammatory bowel diseases such as ulcerative colitis are associated with increase in the colonization intestine reducing bacteria along production. Consistent its increased production, implicated mediator both genesis or maintenance. As T cells well established mediators inflammatory disease, we...
Signaling through the thrombospondin-1 receptor CD47 broadly limits cell and tissue survival of stress, but molecular mechanisms are incompletely understood. We now show that loss permits sustained proliferation primary murine endothelial cells, increases asymmetric division enables these cells to spontaneously reprogram form multipotent embryoid body-like clusters. c-Myc, Klf4, Oct4 Sox2 expression is elevated in CD47-null several tissues CD47- thrombospondin-1-null mice a human T line...
Thrombospondin-1 regulates inflammation by engaging several cell surface receptors and modulating activities of other secreted factors. We have uncovered a novel role thrombospondin-1 in production activation the proinflammatory cytokine IL-1β human murine macrophages. Physiological concentrations limit induction lipopolysaccharide mRNA total protein This inhibition can be explained ability to disrupt interaction between CD47 CD14, thereby limiting NFκB/AP-1 lipopolysaccharide. Only...
Abstract Several types of adrenocortical tumors that lead to Cushing syndrome may be caused by aberrant cyclic AMP (cAMP) signaling. We recently identified patients with micronodular hyperplasia who were carriers inactivating mutations in the 2q-located phosphodiesterase 11A (PDE11A) gene. now studied frequency two missense substitutions, R804H and R867G, conserved regions enzyme several sets normal controls, including 745 individuals enrolled a longitudinal cohort study, New York Cancer...
A variety of adrenal tumors and bilateral adrenocortical hyperplasias (BAH) leading to Cushing syndrome (CS) may be caused by aberrant cAMP signaling. We recently identified patients with a micronodular form BAH that we have called "isolated disease" (iMAD) in whom CS was associated inactivating mutations phosphodiesterase (PDE) 11A (PDE11A). In the present study, examined PDE11A expression normal tissue, sporadic tumors, without mutations, primary pigmented nodular disease (PPNAD) adenomas...
CD47 is a widely expressed receptor that regulates immunity by engaging its counter-receptor SIRPα on phagocytes and secreted ligand thrombospondin-1. Mice lacking can exhibit enhanced or impaired host responses to bacterial pathogens, but role in fungal has not been examined. cd47-/- mice C57BL/6 background showed significantly increased morbidity mortality following Candida albicans infection when compared with wild-type mice. Despite normal colonization at earlier times, four days...
Droplet digital PCR (ddPCR) is being advocated as a reference method to measure rare genomic targets. It has consistently been proven be more sensitive and direct at discerning copy numbers of DNA than other quantitative methods. However, one the largest obstacles measuring microRNA (miRNA) using ddPCR that reverse transcription efficiency depends upon target, meaning small RNA nucleotide composition directly effects primer specificity in manner prevents traditional quantitation optimization...
Reactive estrogen species cause genotoxicity and interfere with docetaxel-mediated tubulin polymerization resulting in shortened survival men castrate-resistant prostate cancer (CRPC).We hypothesized that polymorphisms synthesis targets (i.e., CYP19 ERα) would be linked to interindividual variation CRPC risk, docetaxel response, overall CRPC.Patients (n=115) treated docetaxel, single-agent thalidomide (n=42), or healthy controls (n=289) were genotyped for the R264C (rs700519) ERα PvuII T>C...
We compared different methods (absorbance, fluorescent dye-binding, and digital PCR) for measuring the concentrations of human genomic DNA from cultured cells absorbance measurements a synthetic oligonucleotide. NIST Standard Reference Material (SRM) 2082, pathlength standard, was used to benchmark done with microvolume spectrophotometers plate reader. Control values were measured on high accuracy spectrophotometer calibrated cuvette. Measurements sample several types dye binding assays...
We conducted a multilaboratory assessment to determine the suitability of new commercially available reference material with 40 cancer variants in background wild-type DNA at four different variant allele frequencies (VAFs): 2%, 0.50%, 0.125%, and 0%. The include single nucleotides, insertions, deletions, two structural variations selected for their clinical importance challenge performance next-generation sequencing (NGS) methods. Fragmented was formulated simulate size distribution...
Platelet-generated thromboxane A2 enhances norepinephrine release from adrenergic nerves. Microsomes were prepared outdated human platelets and incubated with arachidonic acid to produce A2. The incubate was added isolated, electrically stimulated rabbit portal veins determine its effect on neurotransmission. Immunoreactive B2 concentrations measured assess the generation of precursor, Addition microsomes in presence (1 microM) caused a concentration-dependent increase concentrations,...
The effect of alpha adrenergic receptor antagonists on concentration-dependent response to angiotensins II and III was examined in the electrically stimulated isolated rabbit vas deferens. force generated by a nonadrenergic neural mechanism reduced both peptides whereas mechanisms enhanced. Angiotensin III-induced inhibition contraction significantly greater than that angiotensin for all groups. Yohimbine (1 X 10(-4) M), an alpha-2 antagonist, attenuated depression produced III. 10(-5) 1 M)...