A5056213262- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- RNA Research and Splicing
- DNA Repair Mechanisms
- NF-κB Signaling Pathways
- Histone Deacetylase Inhibitors Research
- Tardigrade Biology and Ecology
- RNA regulation and disease
- Educational Practices and Policies
- Estrogen and related hormone effects
- Spaceflight effects on biology
- Animal Genetics and Reproduction
- Protein Degradation and Inhibitors
- Bone Metabolism and Diseases
- Advanced biosensing and bioanalysis techniques
- Heat shock proteins research
- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- Pluripotent Stem Cells Research
- Acute Myeloid Leukemia Research
- Natural Compounds in Disease Treatment
- Cell death mechanisms and regulation
University of California, San Diego
2017-2025
Universidad Nacional Autónoma de México
2012-2022
Google (United States)
2016
Tardigrades, also known as water bears, are animals that can survive extreme conditions. The tardigrade Ramazzottius varieornatus contains a unique nuclear protein termed Dsup, for damage suppressor, which increase the resistance of human cells to DNA under conditions, such ionizing radiation or hydrogen peroxide treatment, generate hydroxyl radicals. Here we find R. Dsup is nucleosome-binding protects chromatin from Moreover, ortholog Hypsibius exemplaris similarly binds nucleosomes and...
General transcription is required for the growth and survival of all living cells. However, tumor cells require extraordinary levels transcription, including ribosomal RNA genes by polymerase I (RNPI) mRNA II (RNPII). In fact, cancer have mutations that directly enhance are frequently transformation. For example, recent discovery MYC enhances majority in genome correlates with fact several interfering drugs preferentially kill years, advances mechanistic studies basal machinery interfere...
The nuclear receptor corepressor (NCoR) forms a complex with histone deacetylase 3 (HDAC3) that mediates repressive functions of unliganded receptors and other transcriptional repressors by deacetylation substrates. Recent studies provide evidence NCoR/HDAC3 complexes can also exert coactivator in brown adipocytes deacetylating activating PPARγ 1α (PGC1α) signaling via activator factor kappa-B (RANK) promotes the formation stable NCoR/HDAC3/PGC1β coactivates (NFκB)- protein 1...
The tardigrade damage suppressor (Dsup) and vertebrate high mobility group N (HMGN) proteins bind specifically to nucleosomes via a conserved motif whose structure has not been experimentally determined. Here we used cryo-EM show that both the nucleosome acidic patch analogous arginine anchors with one molecule bound each face of nucleosome. We additionally employed natural promoter-containing 5S rDNA sequence for structural analysis These structures an ancient nucle-osome-binding suggest...
The nuclear receptor co-repressor (NCoR) complex mediates transcriptional repression dependent on histone deacetylation by deacetylase 3 (HDAC3) as a component of the complex. Unexpectedly, we found that signaling activator factor κB (RANK) converts NCoR/HDAC3 to co-activator AP-1 and NF-κB target genes are required for mouse osteoclast differentiation. Accordingly, dominant function complexes in response RANK is activate, rather than repress, gene expression. Mechanistically, promotes...
A key challenge in precise genome editing is the low efficiency of homology-directed repair (HDR). Here we describe a strategy for increasing HDR cells by using chromatin donor template instead naked DNA template. The use chromatin, which natural form nucleus, increases frequency HDR-edited clones as well homozygous editing. In addition, transfection results negligible cytotoxicity. These findings suggest that should be useful wide range applications such insertion or replacement fragments...
TFIIH participates in transcription, nucleotide excision repair and the control of cell cycle. In this work, we demonstrate that Dmp52 subunit Drosophila physically interacts with fly p53 homologue, Dp53. The depletion wing disc generates chromosome fragility, increases apoptosis produces wings a reduced number cells; cellular proliferation, however, is not affected. Interestingly, instead suppressing apoptotic phenotype, Dp53 Dmp52-depleted cells suffer from fragility. induced by alone...
The multisubunit DNA repair and transcription factor TFIIH maintains an intricate cross-talk with different factors to achieve its functions. p8 subunit of the basal levels complex by interacting p52 subunit. Here, we report that in Drosophila, homolog (Dmp8) is encoded a bicistronic transcript Swc6/p18(Hamlet) (Dmp18) SWR1/SRCAP chromatin remodeling complex. SWR1 SRCAP complexes catalyze exchange canonical histone H2A H2AZ variant. In eukaryotic cells, transcripts are not common, some...
ABSTRACT In Drosophila, zygotic genome activation occurs in pre-blastoderm embryos during rapid mitotic divisions. How the transcription machinery is coordinated to achieve this goal a very brief time span still poorly understood. Transcription factor II H (TFIIH) fundamental for initiation by RNA polymerase (RNAPII). Herein, we show vivo dynamics of TFIIH at onset Drosophila embryos. shows an oscillatory behaviour between nucleus and cytoplasm. foci are observed from interphase metaphase,...
Eukaryotic gene expression is activated by factors that interact within complex machinery to initiate transcription. An important component of this the DNA repair/transcription factor TFIIH. Mutations in TFIIH result three human syndromes: xeroderma pigmentosum, Cockayne syndrome and trichothiodystrophy. Transcription repair defects have been linked some clinical features these syndromes. However, how mutations affect specific developmental programmes, allowing organisms develop with...
Chromatin, rather than plain DNA, is the natural substrate of molecular machines that mediate DNA-directed processes in nucleus. Chromatin can be reconstituted vitro by using different methodologies. The salt dialysis method yields chromatin consists purified histones and DNA. This biochemically pure well-suited for a wide range applications. Here, we describe simple straightforward protocols reconstitution stepwise analysis micrococcal nuclease (MNase) digestion assay. with this used...
Alternatives to the cap mechanism in translation are often used by viruses and cells allow them synthesize proteins events of stress viral infection. In Drosophila there hundreds polycistronic messenger RNA (mRNA), various mechanisms known achieve this. However, a same mRNA work cellular mechanism, this is not case for Drosophila's Swc6/p18Hamlet homolog Dmp18, part SWR1 chromatin remodeling complex, who encoded bicistronic next Dmp8 (Dmp8-Dmp18 transcript), structural component...