A5006240770- Colorectal Cancer Treatments and Studies
- Neuroscience and Neuropharmacology Research
- Molecular Biology Techniques and Applications
- Cancer Genomics and Diagnostics
- Amino Acid Enzymes and Metabolism
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced biosensing and bioanalysis techniques
- Neurogenesis and neuroplasticity mechanisms
- Nanocluster Synthesis and Applications
- Reproductive tract infections research
- Tracheal and airway disorders
- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- Colorectal Cancer Screening and Detection
- Biosensors and Analytical Detection
- HER2/EGFR in Cancer Research
- Cardiovascular Health and Disease Prevention
- Machine Learning in Bioinformatics
- Cancer Treatment and Pharmacology
- Bacterial Identification and Susceptibility Testing
- Drug Transport and Resistance Mechanisms
- Global Public Health Policies and Epidemiology
- Metabolism and Genetic Disorders
- Ion channel regulation and function
- Medicinal Plants and Bioactive Compounds
University of Birmingham
2024
University of Freiburg
2024
UNSW Sydney
2020-2024
Emory University
2024
Colorado State University
2024
UK Health Security Agency
2024
The University of Queensland
2024
Washington University in St. Louis
2024
Agency for Science, Technology and Research
2024
University of Edinburgh
2017-2020
Abstract The influence that neurons exert on astrocytic function is poorly understood. To investigate this, we first developed a system combining cortical and astrocytes from closely related species, followed by RNA-seq in silico species separation. This approach uncovers wide programme of neuron-induced gene expression, involving Notch signalling, which drives maintains maturity neurotransmitter uptake function, conserved human development, disrupted neurodegeneration. Separately, hundreds...
To determine the maximum-tolerated dose, toxicities, and pharmacokinetic profile of farnesyl protein transferase inhibitor R115777 when administered orally bid for 5 days every 2 weeks.Twenty-seven patients with a median age 58 years received 85 cycles using an intrapatient interpatient dose escalation schema. Drug was at escalating doses as solution (25 to 850 mg bid) or pellet capsules (500 1300 bid). Pharmacokinetics were assessed after first last during cycle 1.Dose-limiting toxicity...
Methods are described for perfusing (in the dog):— (1) A head‐thorax‐preparation in which systemic and pulmonary circulations supplied by separate pumps, blood being oxygenated isolated perfused lungs from another animal; (2) entire animal two outlet connexions of tied into artery ascending aorta; (3) animal, less lower limbs, pumps one is abdominal aorta other inserted right ventricle. In all three preparations an increase flow gives rise to a slight but definite fall arterial pressure or...
The release of glutamine from astrocytes adjacent to synapses in the central nervous system is thought play a vital role mechanism glutamate recycling and therefore important for maintaining excitatory neurotransmission. Here we investigate nature astrocytic membrane transport rat brainstem slices, using electrophysiological recording fluorescent imaging pHi . Glutamine application perisynaptic induced current, caused by activation A (SA) family transporters. significant electroneutral...
Astrocytes are glial cells that have an intimate physical and functional association with synapses in the brain. One of their main roles is to recycle neurotransmitters glutamate gamma-aminobutyric acid (GABA), as a component glutamate/GABA-glutamine cycle. They perform this function by sequestering releasing glutamine via neutral amino transporter SNAT3. In way, astrocytes regulate availability subsequently influence synaptic function. Since many plasma membrane transporters regulated...
The development of Subzymes demonstrates how the catalytic activity DNAzymes can be controlled for detecting nucleic acids; however, alone lack sensitivity required to detect low target concentrations. To improve sensitivity, we developed a feedback system using pair cross-catalytic Subzymes. These were individually tethered microparticles (MP) and separated by porous membrane rendering them unable interact. In presence target, active PlexZymes® cleave first Subzyme, which separates DNAzyme...
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The probe technology described in this paper facilitates detection and discrimination of multiple targets a single fluorescent channel during PCR. This provides strategy for doubling the number that can be analysed simultaneously on existing PCR instruments. These probes are referred to as PlexProbes produce fluorescence switched ‘on’ or ‘off’ presence target by manipulating temperature. During PCR, measured at temperatures allowing specific defined temperatures. In channel, model duplex...
<h3>Background</h3> Major challenges in the management of infectious diseases include treatment failure due to antimicrobial resistance (AMR) and lack a reliable test cure (TOC). Whilst culture is trusted method it slow, with widespread use nucleic acid amplification tests (NAATs), many labs no longer retain capabilities. NAATs can assess AMR by detecting microbial mutations associated resistance; however, this approach requires knowledge molecular mechanism(s), as new emerge, need be...
Abstract Introduction The MAPK/ERK pathway is a complex signaling cascade involved in many cancer types. KRAS and BRAF gene mutations are present number of cancers, including colon, lung pancreas, identification these genes great importance clinical diagnostics. Moreover, there growing demand for performing multiple tests simultaneously on single sample an increased need to provide answers oncologists short timeframe. Methods IdyllaTM fully integrated automated molecular diagnostics platform...
Abstract Mutations in RAS, RAF, EGFR and PIK3CA genes are present a number of cancers including colon, lung breast cancers. Importantly, many these mutations predictive biomarkers clinical outcomes for targeted therapies. This has led to growing demand detection multiple simultaneously. Multiplex formats allow greater amount information be obtained from each sample. PASS primer technology can sensitively specifically identify the presence SNPs somatic DNA changes (point mutations, deletions...