A5042652072- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- HIV Research and Treatment
- Animal Disease Management and Epidemiology
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Virus-based gene therapy research
- T-cell and Retrovirus Studies
- Viral Infections and Immunology Research
- Vector-Borne Animal Diseases
- RNA Interference and Gene Delivery
- Immune Cell Function and Interaction
- Plant Virus Research Studies
- RNA regulation and disease
- HIV/AIDS drug development and treatment
- MicroRNA in disease regulation
- Animal Virus Infections Studies
- Cancer-related gene regulation
- Viral gastroenteritis research and epidemiology
- Genomics and Chromatin Dynamics
- Sarcoma Diagnosis and Treatment
- PI3K/AKT/mTOR signaling in cancer
- Circular RNAs in diseases
- Chromosomal and Genetic Variations
- Herpesvirus Infections and Treatments
University of Minnesota
2016-2025
The Ohio State University
2008-2020
Twin Cities Orthopedics
2018
Yale University
2011
Comprehensive Blood & Cancer Center
2010
U-M Rogel Cancer Center
2009
The Ohio State University Comprehensive Cancer Center – Arthur G. James Cancer Hospital and Richard J. Solove Research Institute
2003-2005
State Library of Ohio
2002
Institut thématique Génétique, génomique et bioinformatique
2002
University of Wisconsin–Madison
1993-1995
Retroviruses rely on host RNA-binding proteins to modulate various steps in their replication. Previously several animal retroviruses were determined mediate Dhx9/RNA helicase A (RHA) interaction with a 5' terminal post-transcriptional control element (PCE) for efficient translation. Herein PCE reporter assays HTLV-1 and HIV-1 RU5 confer orientation-dependent activity. The effect of Dhx9/RHA down-regulation rescue siRNA-resistant RHA expression HIV-1(NL4-3) provirus that is necessary RNA...
The stem cell protein Lin28 functions to inhibit the biogenesis of a group miRNAs but also stimulates expression subset mRNAs at post-transcriptional level, underlying mechanism which is not yet understood. Here we report characterization molecular interplay between and RNA helicase A (RHA) known play an important role in remodeling ribonucleoprotein particles during translation. We show that reducing results decreased RHA association with polysomes while increasing leads elevated...
Translation is a regulated process and pivotal to proper cell growth homeostasis. All retroviruses rely on the host translational machinery for viral protein synthesis thus may be susceptible its perturbation in response stress, co-infection, and/or cycle arrest. HIV-1 infection arrests G2/M phase, potentially disrupting regulation of translation. In this study, we present evidence that downregulates translation lymphocytes, attributable arrest induced by accessory Vpr. The molecular basis...
The RNA silencing pathway is an intracellular innate response to virus infections and retro-transposons. Many plant viruses counter this host restriction by suppressor (RSS) activity of a double-stranded RNA-binding protein, e.g., tomato bushy stunt P19. Here, we demonstrate P19 HIV-1 Tat function across the animal kingdoms suppress common step in that downstream small maturation. Our experiments reveal infected human cells severely attenuates translational output unspliced gag mRNA,...
ABSTRACT RNA-templated RNA replication is essential for viral or viroid infection, as well regulation of cellular gene expression. Specific motifs likely regulate various aspects this replication. Viroids the Pospiviroidae family, represented by Potato spindle tuber (PSTVd), replicate in nucleus utilizing DNA-dependent polymerase II. We investigated role loop E (sarcin/ricin) motif PSTVd genomic A tertiary-structural model motif, inferred comparative sequence analysis and comparison with...
Appended to the 5' end of nascent RNA polymerase II transcripts is 7-methyl guanosine (m7G-cap) that engages nuclear cap-binding complex (CBC) facilitate messenger (mRNA) maturation. Mature mRNAs exchange CBC for eIF4E, rate-limiting translation factor controlled through mTOR. Experiments in immune cells have now documented HIV-1 incompletely processed exhibited hypermethylated m7G-cap and down-regulation trimethylguanosine synthetase-1-reduced infectivity virion protein synthesis by several...
Abstract Background MicroRNA (miRNA)-mediated RNA silencing is integral to virtually every cellular process including cell cycle progression and response virus infection. The interplay between HIV-1 multifaceted, accumulating evidence posits a strike-counterstrike interface that alters the environment favor replication. For instance, miRNA-mediated of translation antagonized by Tat suppressor activity. activity accessory proteins Vpr/Vif delays progression, which prominently modulated miRNA....
ABSTRACT The retroviral primary transcription product is a multifunctional RNA that utilized as pre-mRNA, mRNA, and genomic RNA. relationship between human immunodeficiency virus type 1 (HIV-1) unspliced transcripts used mRNA for viral protein synthesis virion precursor (vpRNA) encapsidation remains an important question. We developed biochemical assay to evaluate the hypothesis prior utilization template necessary generate vpRNA. HIV-1-infected T cells were treated with translation...
The 5′ untranslated region (UTR) of retroviruses contain structured replication motifs that impose barriers to efficient ribosome scanning. Two RNA structural facilitate translation initiation despite a complex UTR are internal entry site (IRES) and proximal post-transcriptional control element (PCE). Here, stringent protein analyses determined the spleen necrosis virus (SNV), reticuloendotheliosis A (REV-A) human T-cell leukemia type 1 (HTLV-1) exhibit PCE activity, but not IRES activity....
The paradigm protein synthesis rate is regulated by structural complexity of the 5'untranslated region (UTR) derives from bacterial and other riboswitches. In-solution, HIV-1 5'UTR forms two interchangeable long-range nucleotide (nt) -pairings, one sequesters gag start codon promoting dimerization while dimer initiation signal preventing dimerization. While effect these nt-pairings on packaging has been documented their authentic HIV translation in cellulo remained elusive until now....
One long-standing knowledge gap is the role of nuclear proteins in mRNA translation. Nuclear RNA helicase A (DHX9/RHA) necessary for translation mRNAs JUND (JunD proto-oncogene AP-1 transcription factor subunit) and HIV-1 genes, cap-binding protein 1 (NCBP1)/CBP80 a component polysomes. The kinase mTOR activates canonical messenger ribonucleoproteins by post-translationally down-regulating eIF4E inhibitory 4E-BP1. We posited here that NCBP1 DHX9/RHA (RHA) support pathway independent mTOR....
ABSTRACT Previous work has shown that spleen necrosis virus (SNV) long terminal repeats (LTRs) are associated with Rex/Rex-responsive element-independent expression of bovine leukemia RNA and supports the hypothesis SNV contains a cis -acting element interacts cellular Rex-like proteins. To test this hypothesis, human immunodeficiency type 1 (HIV) Rev/RRE-dependent gag gene was used as reporter to analyze various sequences. Gag enzyme-linked immunosorbent assay Western blot analyses reveal...