A5036683032- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA regulation and disease
- Cancer-related molecular mechanisms research
- Estrogen and related hormone effects
- Molecular Biology Techniques and Applications
- Reproductive biology and impacts on aquatic species
- RNA Interference and Gene Delivery
- Monoclonal and Polyclonal Antibodies Research
- Mass Spectrometry Techniques and Applications
- Genomics and Chromatin Dynamics
- Muscle Physiology and Disorders
- Advanced Proteomics Techniques and Applications
- Signaling Pathways in Disease
- Viral Infections and Immunology Research
- DNA and Nucleic Acid Chemistry
- Biotin and Related Studies
- Protein purification and stability
- Drug-Induced Hepatotoxicity and Protection
- Nuclear Structure and Function
- Endoplasmic Reticulum Stress and Disease
- Neurobiology and Insect Physiology Research
- Effects and risks of endocrine disrupting chemicals
- Hormonal and reproductive studies
The Ohio State University
2011-2021
Columbus Center
2020
American Airlines (United States)
2018
Institut de Biologie Moléculaire et Cellulaire
2011
State Library of Ohio
2004
Roswell Park Comprehensive Cancer Center
2002
Cancer Genetics (United States)
2002
Oak Ridge National Laboratory
2002
Guy's Hospital
2002
University of Illinois Urbana-Champaign
2000
Although papillary thyroid carcinoma (PTC) displays strong heritability, no predisposing germ-line mutations have been found. We show that a common G/C polymorphism (rs2910164) within the pre-miR-146a sequence reduced amount of pre- and mature miR-146a from C allele 1.9- 1.8-fold, respectively, compared with G allele. This is matched by similar decrease in each pre-miR generated corresponding pri-miR-146a an vitro processing reaction. The also interfered binding nuclear factor to...
Contact of Mycobacterium tuberculosis (M.tb) with the immune system requires interactions between microbial surface molecules and host pattern recognition receptors. Major M.tb-exposed cell envelope molecules, such as lipomannan (LM), contain subtle structural variations that affect nature response. Here we show LM from virulent M.tb (TB-LM), but not avirulent Myocobacterium smegmatis (SmegLM), is a potent inhibitor TNF biosynthesis in human macrophages. This difference response because...
4E-transporter (4E-T) is one of several proteins that bind the mRNA 5'cap-binding protein, eukaryotic initiation factor 4E (eIF4E), through a conserved binding motif. We previously showed 4E-T nucleocytoplasmic shuttling which mediates import eIF4E into nucleus. At steady state, predominantly cytoplasmic and concentrated in bodies conspicuously resemble recently described processing (P-bodies), are believed to be sites decay. In this paper, we demonstrate colocalizes with decapping factors...
Endonuclease decay of nonsense-containing -globin mRNA in erythroid cells generates 5-truncated products that were reported previously to have a cap or caplike structure.We confirmed this 5 modification is indistinguishable from the on full-length mRNA, and Western blotting, immunoprecipitation, active-site labeling identified population capping enzymes cytoplasm nonerythroid cells.Cytoplasmic enzyme sediments 140-kDa complex contains kinase which, together with enzyme, converts...
Cap homeostasis is a cyclical process of decapping and recapping that impacts portion the mRNA transcriptome. The metastable uncapped forms targets redistribute from polysomes to non-translating mRNPs, all needed for their return translating pool. Previous work identified cytoplasmic capping metabolon consisting enzyme (CE) 5′-monophosphate kinase bound adjacent domains Nck1. current study identifies canonical cap methyltransferase (RNMT) as responsible guanine-N7 methylation recapped mRNAs....
In previous studies, the induction of Ia antigens on murine peritoneal exudate macrophages by recombinant IFN-gamma (rIFN-gamma) and antagonism rIFN-gamma-induced expression inhibitors IFN-alpha/beta glucocorticoids have been examined. this report, these findings extended to an analysis total or cytoplasmic mRNA from macrophage cultures treated with rIFN-gamma in absence presence two inhibitors. Recombinant induced a 5.7- 6.5-fold increase steady-state levels (A alpha-specific) mRNA....
In Xenopus , estrogen induces the stabilization of vitellogenin mRNA and destabilization albumin mRNA. These processes correlate with increased polysomal activity a sequence-selective endonuclease, PMR-1, hnRNP K homology-domain RNA-binding protein, vigilin. Vigilin binds to region 3′-untranslated (3′-UTR) implicated in estrogen-mediated stabilization. The vigilin-binding site B1 3′-UTR contains two consensus PMR-1 cleavage sites. availability purified recombinant vigilin made it possible...
mRNA capping and decapping requires a cytoplasmic complex to maintain and/or restore the 5′ cap on subset of mammalian transcriptome; Nck1, an SH2/SH3 adapter, creates scaffold upon which forms.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEstrogen-induced ribonuclease activity in Xenopus liverRicardo L. Pastori, John E. Moskaitis, and Daniel R. SchoenbergCite this: Biochemistry 1991, 30, 43, 10490–10498Publication Date (Print):October 1, 1991Publication History Published online1 May 2002Published inissue 1 October 1991https://pubs.acs.org/doi/10.1021/bi00107a018https://doi.org/10.1021/bi00107a018research-articleACS PublicationsRequest reuse permissionsArticle...
The A؉U-rich elements (or AREs) are cis-acting sequences that activate rapid mRNA decay, yet the overall polarity of this process is unknown.The current study describes an unbiased approach to using Invader RNA assay (Third Wave Technologies, Inc.) quantify decay each three exons human -globin without added instability or with AREs from c-fos granulocyte-macrophage colony-stimulating factor (GM-CSF) in 3 untranslated region.Each these genes under tetracycline operator control was stably...
In previous studies, the induction of Ia Ag on murine macrophages by rIFN-gamma and antagonism this IFN-alpha/beta glucocorticoids were examined. These findings showed that both rIFN-gamma, as well rIFN-gamma-induced expression dexamethasone, are regulated cognate changes in mRNA. present report, observation was first extended to show decreased steady-state level Ia-specific mRNA seen cytoplasm treated with presence or dexamethasone also nuclear RNA preparations. To test hypothesis...
Abstract In search of tumor suppressor genes in papillary thyroid carcinoma (PTC), we previously used gene expression profiling to identify underexpressed compared with paired unaffected tissue. While searching for loss heterozygosity (LOH) genomic regions harboring candidate genes, detected LOH a ∼20 kb region around marker D9S176 . Several ESTs flanking were PTC tumors, and one the ESTs, downregulation was highly associated activating BRAF mutation V600E, most common genetic lesion PTC. A...
A previous report from this laboratory described an estrogen-regulated endoribonuclease activity on Xenopus liver polysomes which had properties one might expect for a messenger ribonuclease involved in the regulated destabilization of albumin mRNA (Pastori, R. L., Moskaitis, J. E., and Schoenberg, D. R.(1991) Biochemistry 30, 10490-10498). This describes purification ribonuclease. The purified nuclease fraction contained doublet 62 64 kDa small amount 40-kDa peptide. In situ analysis both...
Pentraxins are a family of acute phase reactants. Two members, C-reactive protein (CRP) and serum amyloid P component (SAP), known in range mammalian species. CRP SAP both about 200 residues long, arose from gene duplication event, apparently before the divergence orders. To elucidate origins pentraxins, we have searched for pentraxin-coding genes amphibian Xenopus laevis. We identified determining (XL-PXN1) which is twice size expected: XL-PXN1 appears to be fusion between regions encoding...
Previous work showed that human beta-globin mRNAs harboring a premature termination codon are degraded in the erythroid tissues of mice to products lack sequences from mRNA 5' end but contain cap-like structure. Whether these decay consequence endonucleolytic or 5'-to-3' exonucleolytic activity is unclear. We report this pathway recapitulated cultured mouse erythroleukemia (MEL) cells and targets nonsense-free lesser extent than nonsense-containing mRNA. S1 nuclease mapping primer extension...