Summary Objective To identify and understand the natural transmission cycles of Leishmania in Iranian sandflies. Method Nested PCR protocols were developed to amplify two regions ribosomal RNA amplicon (ITS1‐ 5.8S rRNA gene, a microsatellite DNA region ITS2), which species‐specific by sequence or fragment size. Results The assays detected sandflies not only major but also for first time L. turanica gerbilli sensu lato , other parasites great gerbil. All three found northeast centre Iran,...

Abstract. In Iran, Phlebotomus papatasi (Scopoli) (Diptera: Psychodidae) is the main vector of Leishmania major Yakimoff & Schokhor (Kinetoplastida: Trypanosomatidae), causative agent rural zoonotic cutaneous leishmaniasis. This sandfly abundant both in villages and burrows reservoir host, gerbil Rhombomys opimus (Licht.) (Rodentia: Gerbillidae). Populations P. were sampled from edges Isfahan province, using CDC miniature light traps peridomestic sites sticky papers placed at entrances...

Cutaneous leishmaniasis has various phenotypic aspects consisting of polymorphic amastigotes with different genetic ranges. Samples were collected from suspected patients Khuzestan province. Prepared smears stained, scaled, and measured using ocular micrometer. The Cyt b , ITS-rDNA, microsatellite genes Leishmania amplified species identified by molecular analyses. Of 150 examined patients, 102 to (90 L. major nine tropica three unidentified). 90 had regular irregular shapes within clinical...

To assess the effectiveness of community-wide deployment insecticide-impregnated collars for dogs- reservoir Leishmania infantum-to reduce infantile clinical visceral leishmaniasis (VL).

Summary Sergentomyia sintoni is the natural vector of Sauroleishmania species lizards. This sandfly abundance in and around burrows great gerbils. S. was collected from peridomestic animal shelters, inside houses also nearby gerbil reservoir hosts, Rhombomys opimus, several provinces Iran. Mitochondrial Cytochrome b (Cyt b) sandflies, which a maternally-inherited gene marker used to characterize different haplotypes populations this sandfly. The analyses were based on last 717 bp Cyt...

Journal Article Two Leishmania species circulating in the Kaleybar focus of infantile visceral leishmaniasis, northwest Iran: implications for deltamethrin dog collar intervention Get access P. Parvizi, Parvizi aDepartment Parasitology, Pasteur Institute Iran, Tehran, IranbDepartment Entomology, Natural History Museum, Cromwell Road, London SW7 5BD, UK Search other works by this author on: Oxford Academic PubMed Google Scholar A.S. Mazloumi-Gavgani, Mazloumi-Gavgani cDepartment University...

Abstract Diagnostic molecular markers for the females of Phlebotomus ( Paraphlebotomus ) caucasicus and P. mongolensis were sought by characterizing from individual Iranian specimens a gene fragment, namely mitochondrial cytochrome b , that had previously proven useful taxonomy phlebotomine sandflies. Males both species used as reference material because their external genitalia provide only diagnostic morphological characters. A phylogenetic analysis new sequences, those reported grimmi...

In the well-known zoonotic cutaneous leishmaniasis (ZCL) focus in Turkmen Sahara, border of Iran and Turkmenistan, ZCL has increased among humans past five years. The present study was undertaken to incriminate vectors region, find molecular variation Leishmania parasites.The sandflies were sampled using CDC light-traps sticky papers. All identified morphological characters head abdominal terminalia. DNA extracted from dissected thorax attached anterior abdomen individual female sandfly....

To assess molecular characterization, distribution, seasonal activities of sandfly species and Leishmania parasites infecting them for this zoonotic cutaneous leishmaniasis focus.The collections were carried out in 2009-2011 using CDC traps, Sticky Papers manual aspirator around the villages Abarkouh district. Individual sandflies characterized by PCR amplification sequencing fragments their mitochondrial cytochrome b gene. parasite infections within performed targeting Cyt b, ITS-rDNA,...

Journal Article Low diversity of Leishmania parasites in sandflies and the absence great gerbil foci zoonotic cutaneous leishmaniasis Fars province, southern Iran Get access P. Parvizi, Parvizi aMolecular Systematics Laboratory, Pasteur Institute Iran, Tehran, Islamic Republic Search for other works by this author on: Oxford Academic PubMed Google Scholar E. Alaeenovin, Alaeenovin P.A. Kazerooni, Kazerooni bShiraz University Medical Sciences, Shiraz, P.D. Ready * cDisease Control Department,...

Individual, naturally occurring Phlebotomus mongolensis and caucasicus from Iran were screened for infections with the maternally inherited intracellular Rickettsia-like bacterium Wolbachia pipientis via targeting a major surface protein gene (wsp). The main objective of this study was to determine if W. could be detected in these species. sandflies using polymerase chain reaction amplify fragment gene. obtained sequences edited aligned database identify haplotypes. Two strains found. Strain...

Abstract Objectives Only L eishmania major is well known as a causative agent of zoonotic cutaneous leishmaniasis ( ZCL ) in I ran. Our objective was to find parasites circulating reservoir hosts, sand flies and human simultaneously. Methods Sand flies, rodents prepared smears humans were sampled. DNA extracted, two fragments ITS ‐ rDNA gene amplified by PCR . RFLP sequencing employed identify parasites. Results turanica identified unequivocally targeting from humans, flies. The new species...

Abstract Background The characterization of Leishmania species is important for clinical management the diseases and epidemiological control parasite distribution. Most published polymerase chain reaction (PCR) amplification methods lack ability to identify all complexes, have low performance usually need post-PCR procedures. There a improving diagnosis CL by development an accurate affordable PCR method in specimens. Methods We designed optimized amplifying internal transcribed spacer 2...