A5080674738- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Transgenic Plants and Applications
- Plant and Fungal Interactions Research
- Insect-Plant Interactions and Control
- Plant Parasitism and Resistance
- Plant Disease Resistance and Genetics
- Plant Pathogenic Bacteria Studies
- Insect symbiosis and bacterial influences
- Legume Nitrogen Fixing Symbiosis
- Bacteriophages and microbial interactions
- Mosquito-borne diseases and control
- CRISPR and Genetic Engineering
- Photosynthetic Processes and Mechanisms
- Plant Pathogens and Resistance
- RNA Interference and Gene Delivery
- Plant Disease Management Techniques
- Cancer Research and Treatments
- Virology and Viral Diseases
- Yeasts and Rust Fungi Studies
- Chromosomal and Genetic Variations
- Fungal and yeast genetics research
- Agronomic Practices and Intercropping Systems
- Bacterial Genetics and Biotechnology
University of Missouri
2014-2025
Plant (United States)
1994-2018
Missouri State University
2014
Ningxia Academy of Agriculture and Forestry Sciences
2014
GTx (United States)
1999-2012
Seoul Women's University
2011
Friedrich Miescher Institute
2008
Noble Research Institute
2008
Waters (United Kingdom)
2006
University of Kentucky
1986-1990
Summary A new allele of the coronatine‐insensitive locus ( COI1 ) was isolated in a screen for Arabidopsis thaliana mutants with enhanced resistance to bacterial pathogen Pseudomonas syringae . This mutant, designated coi1‐20 , exhibits robust several P. isolates but remains susceptible virulent pathogens Erisyphe and cauliflower mosaic virus. Resistance strain Pst DC3000 plants is correlated hyperactivation PR‐1 expression accumulation elevated levels salicylic acid (SA) following...
A domain of cauliflower mosaic virus (CaMV) which controls systemic spread in two solanaceous hosts (Datura stramonium and Nicotiana bigelovii) was mapped to the first half open reading frame 6. Whereas ordinary strains CaMV are unable infect species except replicate locally inoculated leaves, a new strain (D4) induces chlorotic local lesions systemically infects both D. N. bigelovii. To determine portion genome hosts, nine recombinant genomes constructed between D4 were tested for their...
Abstract The gene VI product (P6) of Cauliflower mosaic virus (CaMV) is a multifunctional protein known to be major component cytoplasmic inclusion bodies formed during CaMV infection. Although these inclusions are contain virions and thought sites translation from the 35S polycistronic RNA intermediate, precise role in infection cycle remains unclear. Here, we examine functionality intracellular location fusion between P6 GFP (P6-GFP). We initially show that ability P6-GFP transactivate...
Several RNA silencing pathways in plants restrict viral infections and are suppressed by distinct proteins. Here we show that the endogenous trans-acting (ta)siRNA pathway, which depends on Dicer-like (DCL) 4 RNA-dependent polymerase (RDR) 6, is infection of Arabidopsis with Cauliflower mosaic virus (CaMV). This effect was associated overaccumulation unprocessed, RDR6-dependent precursors tasiRNAs due solely to expression CaMV transactivator/viroplasmin (TAV) protein. TAV also impaired...
We used PCR to differentiate species in the genus Phytophthora, which contains a group of devastating plant pathogenic fungi. focused on Phytophthora parasitica, that can infect solanaceous plants such as tomato, and citrophthora, is primarily citrus pathogen. Oligonucleotide primers were derived from sequences 1,300-bp P. parasitica-specific DNA segment an 800-bp citrophthora-specific segment. Under optimal conditions, developed for parasitica specifically amplified 1,000-bp sequence...
We have shown previously that gene VI of cauliflower mosaic virus (CaMV) strain D4 governs systemic infection Nicotiana bigelovii and transgenic N. expressing the product can complement at least one CaMV isolate for long-distance transport. now found DNA two other isolates recombine with coding sequence present in plants. The formation recombinant viruses occurs as a consequence replication, involving template switches during reverse transcription RNA to DNA. first switch 5[prime] end 35S...
Cauliflower mosaic virus strain W260 elicits a hypersensitive response (HR) in leaves of Nicotiana edwardsonii, an interspecific hybrid derived from cross between N. glutinosa and clevelandii. Interestingly, we found that is resistant to W260, but responds with local chlorotic lesions rather than necrotic lesions. In contrast, clevelandii systemic cell death. The reactions the progenitors edwardsonii infection indicated each contributed factor toward development HR. this study, present two...
We demonstrate that recombinant viruses formed between a wild-type virus and viral transgene can be isolated from transgenic plants under conditions of moderate to weak selection pressure. inoculated cauliflower mosaic (CaMV) strain W260 transgenicNicotiana bigeloviiplants expressed copy CaMV gene VI derived D4, determines systemic infection solanaceous species, includingN. bigelovii.Because infects nontransformedN. bigeloviisystemically, the D4 would expected have little selective advantage...
The P6 protein of Cauliflower mosaic virus (CaMV) is responsible for the formation inclusion bodies (IBs), which are sites viral gene expression, replication, and virion assembly. Moreover, recent evidence indicates that ectopically expressed inclusion-like (I-LBs) move in association with actin microfilaments. Because CaMV virions accumulate preferentially IBs, we hypothesized IBs have a role delivering to plasmodesmata. We determined interacts C2 calcium-dependent membrane-targeting...
Cauliflower mosaic virus (CaMV) strains D4 and W260 can be distinguished by the type of symptoms they induce in Nicotiana clevelandii N. edwardsonii. induces systemic cell death addition to a symptom hypersensitive response (HR) edwardsonii, whereas both hosts. To determine which genes are responsible for death, chimeric viruses were constructed between strains. It was found that gene VI elicitation death; previous studies had shown this same elicited HR An immunological analysis plants...
Cauliflower mosaic virus strain W260 induces hypersensitive response (HR) in Nicotiana edwardsonii and systemic cell death N. clevelandii. In contrast, the D4 of evades host defenses species; it chlorotic primary lesions a both hosts. Previous studies with chimeric viruses had indicated that gene VI was responsible for elicitation HR or death. To prove conclusively is responsible, we inserted into Agrobacterium tumefaciens binary vector pKYLX7. Agroinfiltration these constructs leaves...