A5080388346- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Insect-Plant Interactions and Control
- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Plant Disease Management Techniques
- Insect and Pesticide Research
- Plant Parasitism and Resistance
- Plant Disease Resistance and Genetics
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Forest Insect Ecology and Management
- Vector-Borne Animal Diseases
- Plant Pathogens and Fungal Diseases
- Horticultural and Viticultural Research
- Agronomic Practices and Intercropping Systems
- Viral Infections and Vectors
- Chromosomal and Genetic Variations
- Listeria monocytogenes in Food Safety
- Salmonella and Campylobacter epidemiology
- Yeasts and Rust Fungi Studies
- Phytoplasmas and Hemiptera pathogens
- Transgenic Plants and Applications
- Molecular Biology Techniques and Applications
University of California, Davis
2003-2018
Plant (United States)
1988-2015
Marymount University
1998
University of Kentucky
1986
We have characterized the virome in single grapevines by 454 high-throughput sequencing of double-stranded RNA recovered from vine stem. The analysis revealed a substantial set sequences similar to those fungal viruses. Twenty-six putative virus groups were identified plant source. These represented half all known mycoviral families including Chrysoviridae, Hypoviridae, Narnaviridae, Partitiviridae, and Totiviridae. Three mycoviruses associated with Botrytis cinerea, common pathogen grapes....
A bioassay is routinely used to determine the viral phytosanitary status of commercial grapevine propagation material in many countries around world. That test based on symptoms developed field by specific indicator host plants that are graft-inoculated from vines being tested. We compared against next-generation sequencing (NGS) analysis material. NGS a laboratory procedure catalogs genomic sequences viruses and other pathogens extracted as DNA RNA infected vines. was found be superior...
A domain of cauliflower mosaic virus (CaMV) which controls systemic spread in two solanaceous hosts (Datura stramonium and Nicotiana bigelovii) was mapped to the first half open reading frame 6. Whereas ordinary strains CaMV are unable infect species except replicate locally inoculated leaves, a new strain (D4) induces chlorotic local lesions systemically infects both D. N. bigelovii. To determine portion genome hosts, nine recombinant genomes constructed between D4 were tested for their...
Genetic diversity was characterized in 14 isolates of Grapevine fanleaf virus (GFLV) recovered from grapevine ( Vitis vinifera ). Virions were collected by immunocapture, and a 1557 bp fragment containing part the viral coat protein gene untranslated region to its 3′ side amplified RT–PCR. Sequence variation among restriction length polymorphism (RFLP) analysis sequencing. The Ava II-generated RFLP patterns various highly variable. passaged Chenopodium quinoa . altered with passage through...
Mutants of cauliflower mosaic virus (CaMV) strain D4 have been characterized with regard to host-specific phenotypes that resulted from specific changes in the viral DNA sequence. Both mutant and wild-type viruses infect a brassicaceous host, Brassica campestris, systemically, giving indistinguishable symptoms. However, solanaceous host Datura stramonium, which was systemically infectible by virus, mutants induced necrotic local lesions at 21 degrees C above, veinal necrosis lower...
Grapevine fanleaf virus (GFLV) was detected in samples of Bermuda grass (BG) from Iran by reverse transcription-polymerase chain reaction (RT-PCR) using two different pairs GFLV-specific primers, and also enzyme-linked immunosorbent assay (ELISA) antiserum specific for a North American isolate the virus. RT-PCR GFLV both fresh dried BG tissues preparations purified these plants. Cloning sequencing products confirmed that amplified sequences were sections coat protein gene. Similar results...
Diagnostic methods employing the polymerase chain reaction (PCR) provide most sensitive means currently available for detecting viruses in woody plants. A new technique has been tested that does not rely on gel electrophoresis or molecular hybridization to detect virus-specific PCR products. This colorimetric method detection of products from plants was demonstrated be at least as analysis. When combined with immunocapture virions plant sap, provides a apply technology large number samples....