A5083221826- Advanced biosensing and bioanalysis techniques
- Microfluidic and Bio-sensing Technologies
- Medical and Health Sciences Research
- Microfluidic and Capillary Electrophoresis Applications
- Biosensors and Analytical Detection
- DNA and Nucleic Acid Chemistry
- Graphene research and applications
- Nanofabrication and Lithography Techniques
- Advanced Biosensing Techniques and Applications
- Nitric Oxide and Endothelin Effects
- Modular Robots and Swarm Intelligence
- DNA and Biological Computing
- Molecular Biology Techniques and Applications
- Electrowetting and Microfluidic Technologies
- Nanopore and Nanochannel Transport Studies
- Diverse Legal and Medical Studies
- Ion channel regulation and function
- Extracellular vesicles in disease
- Conducting polymers and applications
- Advancements in Photolithography Techniques
- Lipid metabolism and biosynthesis
- Cancer Genomics and Diagnostics
- RNA Interference and Gene Delivery
- Muscle metabolism and nutrition
- Enzyme Catalysis and Immobilization
University of California, San Diego
2013-2025
Oregon Health & Science University
2019-2024
University of California System
2007-2017
La Jolla Bioengineering Institute
2006-2016
Verband der Universitätsklinika Deutschlands
2015
University Hospital Heidelberg
2015
Heidelberg University
2015
Verband der Privaten Krankenversicherung
2015
University of Florida
2012
Max Rubner Institut
2008
Endothelium-derived relaxing factor/nitric oxide (EDRF/NO) synthesized by bovine aortic endothelial cells and subcellular fractions thereof was assayed its stimulating effect on soluble guanylyl cyclase of rat fetal lung fibroblasts (RFL-6 cells). The release EDRF/NO intact could be stimulated with bradykinin, thrombin, or ADP abolished in Ca2(+)-free medium. When were analyzed, some EDRF/NO-synthesizing activity found the cytosolic fraction, but most associated particulate fraction. Both...
We have demonstrated that controlled electric fields can be used to regulate transport, concentration, hybridization, and denaturation of single- double-stranded oligonucleotides. Discrimination among oligonucleotide hybrids with widely varying binding strengths may attained by simple adjustment the field strength. When this approach is used, control allows single base pair mismatch discrimination carried out rapidly (<15 sec) high resolution. Electric takes place at temperatures well...
Exosomes found in the circulation are a primary source of important cancer-related RNA and protein biomarkers that expected to lead early detection, liquid biopsy, point-of-care diagnostic applications. Unfortunately, due their small size (50–150 nm) low density, exosomes extremely difficult isolate from plasma. Current isolation methods time-consuming multistep procedures unlikely translate into To address this issue, we demonstrate ability an alternating current electrokinetic (ACE)...
Pancreatic ductal adenocarcinoma (PDAC) typically has nonspecific symptoms and is often found too late to treat. Because diagnosis of PDAC involves complex, invasive, expensive procedures, screening populations at increased risk will depend on developing rapid, sensitive, specific, cost-effective tests. Exosomes, which are nanoscale vesicles shed into blood from tumors, have come focus as valuable entities for noninvasive liquid biopsy diagnostics. However, rapid capture analysis exosomes...
Microelectronic DNA chip devices that contain planar arrays of microelectrodes have been developed for multiplex hybridization and a variety genomic research diagnostic applications. These are able to produce almost any desired electric field configuration on their surface. This ability well-defined fields allows charged molecules (DNA, RNA, proteins, enzymes, antibodies, nanobeads, even micron scale semiconductor devices) be electrophoretically transported or from microlocation the surface...
Stimulation of soluble guanylyl cyclase in rat fetal lung fibroblasts (RFL-6 cells) was used as a sensitive assay for endothelium-derived relaxing factor/nitric oxide (EDRF/NO) formation. Intact N1E-115 cells released an EDRF/NO-like material that enhanced cyclic GMP levels RFL-6 cells. The synthesis this substance could be stimulated with the receptor agonist neurotensin (10 microM) or by addition EDRF/NO substrate L-arginine (100 microM). In Ca2(+)-free Locke's solution, stimulation...
1. An enzyme that hydrolyses sphingomyelin to ceramide (N-acylsphingosine) and phosphorylcholine was isolated from rat liver. 2. The is particle-bound (mitochondria or lysosomes) can be solubilized by ultrasonic treatment freezing thawing. 3. It has been partially purified precipitation at pH5.2, neutralization ammonium sulphate fractionation. 4. activated Triton X-100 (0.2%) low concentrations of cetyltrimethylammonium bromide (0.02%), higher concentration being inhibitory. 5. optimum pH...
Conventional methods for the isolation of cancer‐related circulating cell‐free (ccf) DNA from patient blood (plasma) are time consuming and laborious. A DEP approach utilizing a microarray device now allows rapid ccf‐DNA directly small volume unprocessed blood. In this study, is used to compare isolated whole plasma 11 chronic lymphocytic leukemia (CLL) patients one normal individual. Ccf‐DNA both samples was separated into high‐field regions, after which cells (blood), proteins, other...
Protease activity is an emerging biomarker for cancer detection as levels are often increased in tumor tissue compared to healthy tissue. Of particular interest the of proteases carried by extracellular vesicle (EV) nanoparticles which oversecreted tumors into circulation. Current methods analyze bound EVs require complex multi-instrument sample processing separate from plasma quantify protease activity. This makes EV-based a challenge diagnostic or point-of-care applications. Here, method...
Abstract Extracellular vesicles (EVs) are small, membrane-bound particles released by all cells that have emerged as an attractive biomarker platform. We study the utility of a dielectrophoretic (DEP) micro-chip device for isolation and characterization EVs derived from plasma specimens patients with brain tumors. were isolated DEP chip subjected to on-chip immunofluorescence (IF) staining determine concentration glial fibrillary acidic protein (GFAP) Tau. analyzed samples independent...
Nanogen's approach to DNA analysis involves the development of a unique active microelectronic device that provides electronic control over variety molecular biological affinity reactions. The core technology is an automated programmable matrix (APEX), which has ability transport, bind and separate charged molecules in electric field generated on surface device. This broad-based platform potentially applicable for multiplexed hybridizations, immunoassays, receptor binding assays, cell typing...
We demonstrate an electrophoretic process for assembling very small devices on a silicon circuit. A 20-μm diameter InGaAs LED was fabricated and then released from the substrate by etching sacrificial layer underneath diode structure. The diode, placed into buffer solution over circuit, positioned onto circuit's tin/lead contact electrodes biasing contacts to establish current in solution. Following removal solution, assembly heated reflow solder. Circuit formation activation is demonstrated...
DNA has been employed to either store digital information or perform parallel molecular computing. Relatively unexplored is the ability combine DNA-based memory and logical operations in a single platform. Here, we show tri-level cell non-volatile system capable of random-access writing bit shifting operations. A microchip with an array individually addressable electrodes was enable random access cells using electric fields. Three segments on template molecule were used encode three data...