A5085369797- Legionella and Acanthamoeba research
- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Pneumonia and Respiratory Infections
- Antifungal resistance and susceptibility
- Pneumocystis jirovecii pneumonia detection and treatment
- Vibrio bacteria research studies
- Antibiotics Pharmacokinetics and Efficacy
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Tuberculosis Research and Epidemiology
- Bacterial Identification and Susceptibility Testing
- Antimicrobial Resistance in Staphylococcus
- Mycobacterium research and diagnosis
- Water Treatment and Disinfection
- Bacterial Infections and Vaccines
- Heme Oxygenase-1 and Carbon Monoxide
- Infectious Diseases and Tuberculosis
- Antibiotic Use and Resistance
- Urinary Tract Infections Management
- Ocular Infections and Treatments
- Parasitic Infections and Diagnostics
- Bacillus and Francisella bacterial research
- Streptococcal Infections and Treatments
- Infectious Diseases and Mycology
- Clostridium difficile and Clostridium perfringens research
University of Pennsylvania
2016-2025
MRC Laboratory of Molecular Biology
2018-2025
University of Cambridge
2018-2025
Faculty of Public Health
2023
Leiden University Medical Center
2023
Dana-Farber Cancer Institute
2023
MRC Epidemiology Unit
2023
Florida College
2022
University of Central Florida
2022
Medical Research Council
2021
The prevalence of antibiotic resistance among extended-spectrum beta-lactamase (ESBL)--producing Escherichia coli and Klebsiella pneumoniae has increased markedly in recent years. Thirty-three patients with infection due to ESBL-producing E. or K. (case patients) were compared 66 matched controls. Total prior use was the only independent risk factor for (odds ratio, 1.10; 95% confidence interval, 1.03--1.18; P=.006). Case treated an effective a median 72 hours after suspected, 11.5 suspected...
Eighteen types of media, all which used charcoal yeast extract medium as a base, were tested for their ability to support the growth stock strain Legionella pneumophila. Fifteen these contained antimicrobial agents. Five antibiotic-containing media performed best in growing then grow L. pneumophila from clinical respiratory tract specimens. One BMPA alpha, cefamandole, polymyxin B, anisomycin, an organic buffer, and alpha-ketoglutarate, contaminated specimens and, conjunction with acid wash...
Tuberculosis has a much shorter incubation period than is widely thought, say <b>Marcel A Behr and colleagues</b>, this implications for prioritising research public health strategies
Klebsiella pneumoniae carbapenemase (KPC)-producing K. is an emerging pathogen with serious clinical and infection control implications. To our knowledge, no study has specifically examined risk factors for KPC-producing or its impact on mortality.To identify colonization pneumoniae, a case-control was performed. Case patients were compared subjects carbapenem-susceptible pneumoniae. A cohort evaluated the association between in-hospital mortality.Fifty-six case 863 identified. In...
People with immunoreactivity to tuberculosis are thought have lifelong asymptomatic infection and remain at risk for active tuberculosis. <b>Marcel A Behr colleagues</b> argue that most of these people no longer infected
▪ Objective: To determine if the leukocyte esterase and bacterial nitrite rapid dipstick test for urinary tract infection (UTI) is susceptible to spectrum bias (when a diagnostic has different sensitivities or specificities in patients with clinical manifestations of disease which intended). Design: Cross-sectional study. Patients: A total 366 consecutive adult whom clinicians performed urinalysis diagnose exclude UTI. Setting: An urban emergency department walk-in clinic. Measurements:...
The incidence of infections due to extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae (ESBL-EK) has increased markedly in recent years. Treatment is difficult because frequent multidrug resistance. Although fluoroquinolones offer effective therapy for ESBL-EK infections, their usefulness threatened by increasing fluoroquinolone To identify risk factors resistance a case-control study all patients with from 1 June 1997 through 30 September 1998 was...
The authors argue that understanding and countering general bacterial mechanisms of phenotypic antibiotic resistance may hold the key to reducing duration treatment all recalcitrant infections, including tuberculosis.
Eighteen patients received tigecycline as treatment for infection due to multidrug-resistant gram-negative bacilli, including Acinetobacter baumannii and Klebsiella pneumoniae carbapenemase- extended-spectrum beta-lactamase-producing Enterobacteriaceae. Pretherapy minimum inhibitory concentration values predicted clinical success. Observed evolution of resistance during therapy raises concern about routine use in such infections when other therapies are available.
Legionnaires' disease was diagnosed in 32 patients with nosocomially acquired pneumonia by using direct immunofluorescence examination of respiratory tract secretions, indirect determinations antibody titer, and/or culture Legionella pneumophila from secretions. Cultures specimens for were positive 13 21 whom appropriate submitted. Using a as definition disease, the sensitivity 62%; that serologic studies, 75%. any test secretions 50%, studies (using all 4 serogroups), 80%; culture, 65%. A...
ABSTRACT Escherichia coli sequence type ST131 (from phylogenetic group B2), often carrying the extended-spectrum-β-lactamase (ESBL) gene bla CTX-M-15 , is an emerging globally disseminated pathogen that has received comparatively little attention in United States. Accordingly, a convenience sample of 351 ESBL-producing E. isolates from 15 U.S. centers (collected 2000 to 2009) underwent PCR-based phylotyping and detection . A total 200 isolates, comprising 4 groups 50 each were (i) negative...
Often, high-sensitivity, point-of-care (POC) clinical tests, such as HIV viral load, require large volumes of plasma. Although centrifuges are ubiquitously used in laboratories to separate plasma from whole blood, centrifugation is generally inappropriate for on-site testing. Suitable alternatives not readily available the relatively milliliters blood that may be needed meet stringent limit-of-detection specifications low-abundance target molecules. We report on a simple-to-use, low-cost,...
A total of 100 water samples, 95% which were taken from hospital potable fixtures, cultured on three different media used for the isolation Legionella pneumophila. The buffered charcoal-yeast extract medium (BCYE alpha medium), BCYE with antimicrobial agents (BMPA and agents, glycine, differential dyes (MWY medium). An acid wash procedure was also specimens plated BMPA media. 24 samples culture positive L. pneumophila by one or more techniques. MWY detected 92% cultures, 83% 79% cultures....
Journal Article Chloramphenicol for the Treatment of Vancomycin-Resistant Enterococcal Infections Get access Anne H. Norris, Norris From Department Medicine, Division Infectious Diseases, Pharmacy Services, and Pathology Laboratory University Pennsylvania Medical Center, Philadelphia, Search other works by this author on: Oxford Academic PubMed Google Scholar Joseph P. Reilly, Reilly Paul Edelstein, Edelstein Patrick J. Brennan, Brennan Mindy G. Schuster Reprints or correspondence: Dr....
Three hundred twenty-four isolates of anaerobic and microaerophilic organisms were examined with a polyvalent direct immunofluorescent antibody (DFA) reagent directed against Legionella pneumophila, serogroups (Sa) I–IV. 53 Bacteroides fragilis cross-reacted the sa I component reagent. Rabbit antisera to three strains L. pneumophila these B. isolates, cross-reactivity was not due preexisting rabbit antibodies; fourth strain did cross-react isolates. Indirect fluorescent (lFA) examinations...
Summary Colonization by methicillin‐resistant Staphylococcus aureus (MRSA) may be persistent in people and is horizontally transmissible. The scientific literature suggests that domestic pets also participate cross‐transmission of MRSA within households. objectives this study were to evaluate the prevalence risk factors for carriage residing households with an MRSA‐infected person. From 66 which patient resided, we screened 47 dogs 52 cats using a swab protocol. Isolates from humans...
Tuberculosis treatment requires months-long combination chemotherapy with multiple drugs, shorter treatments leading to relapses. A major impediment shortening is that