A5062440586- Cancer Research and Treatments
- Sarcoma Diagnosis and Treatment
- Cutaneous Melanoma Detection and Management
- Estrogen and related hormone effects
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related Molecular Pathways
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Soft tissue tumor case studies
- Nanoplatforms for cancer theranostics
- Melanoma and MAPK Pathways
- Neurofibromatosis and Schwannoma Cases
- Cancer Cells and Metastasis
- Cell Adhesion Molecules Research
- Radiopharmaceutical Chemistry and Applications
- Vascular Tumors and Angiosarcomas
- Cancer Diagnosis and Treatment
- Bone Tumor Diagnosis and Treatments
- RNA Interference and Gene Delivery
- Nonmelanoma Skin Cancer Studies
- Helicobacter pylori-related gastroenterology studies
- CAR-T cell therapy research
- Peptidase Inhibition and Analysis
- Neonatal Respiratory Health Research
- melanin and skin pigmentation
University of Illinois Urbana-Champaign
2002-2025
Illinois College
1997-2025
Dr. R. Ahmed Dental College and Hospital
2011-2024
University of Louisville
2024
Chandigarh University
2024
Delhi Technological University
2024
University of Illinois Chicago
2009-2023
University of Delhi
2009-2023
Galgotias University
2020
Post Graduate Institute of Medical Education and Research
2018
Three hundred and three patients with benign solitary neurilemomas, or schwannomas, not associated the classical type of von Recklinghausen's disease were studied. One seventy-two women 131 men. Forty-five percent tumors seen in extremities, no specific area could be related to development these tumors. Approximately 9% occurred soft tissues trunk 14% various unusual sites. Most initially because a painless mass; only 10 complained pain radiating along course peripheral nerve. documented...
From the Gastric and Mixed Tumor Service of Memorial Hospital Division Surgical Oncology University Illinois, Abraham Lincoln School Medicine, Chicago, Illinois Dr. Brasfield is deceased.
Metastatic melanoma is well known for its multiple manifestations, therefore it not uncommon several organ systems to be involved in terminal states. Willis,<sup>1</sup>however, observed that metastasis the gastrointestinal tract rare. This concept challenged by us, since we have found a high incidence of metastatic tract. A review literature shows an antemortem diagnosis stomach<sup>2,3</sup>and small intestine<sup>4</sup>is made occasionally. In most cases, however, was just prior...
From the Gastric and Mixed Tumor Services of Department Surgery, Memorial Hospital for Cancer Allied Diseases James Ewing Hospital, New York City Present address: Division Surgical Oncology, University Illinois College Medicine, Chicago, Illinois.
This first-in-human, phase I clinical trial of p28 (NSC745104), a 28-amino-acid fragment the cupredoxin azurin, investigated safety, tolerability, pharmacokinetics and preliminary activity in patients with p53+ metastatic solid tumours. A total 15 were administered i.v. as short infusion three times per week for 4 weeks followed by 2-week rest under an accelerated titration 3+3 dose escalation design until either grade 3-related adverse event occurred or maximum tolerated (MTD) was reached....
Abstract Azurin, a member of the cupredoxin family copper containing redox proteins, preferentially penetrates human cancer cells and exerts cytostatic cytotoxic (apoptotic) effects with no apparent activity on normal cells. Amino acids 50 to 77 (p28) azurin seem responsible for cellular penetration at least part antiproliferative, proapoptotic against number solid tumor cell lines. We show by confocal microscopy fluorescence-activated sorting that amino 67 (p18) are minimal motif (protein...
A prospective protocol for the management of primary cutaneous melanoma was initiated at University Illinois in October 1968 and continued through June 1974. Over this period 269 cases were treated: 42 head neck region, 75 trunk, 94 lower extremities 58 upper exremities. The levels invasion ranged from II to V, according Clark's classification; level I melanomas excluded. status regional nodes correlated with invasion. Preliminary exploratory celiotomies performed on all patients extremity...
Das Gupta, Tapas K. M.D. Ph.D.; Brasfield, Richard D. M.D.; O'Hara, James Author Information
SCHUMER, WILLIAM M.D.; DAS GUPTA, TAPAS K. MOSS, GERALD S. NYHUS, LLOYD M. M.D. Author Information
Abstract Microorganisms living at many sites in the human body compose a complex and dynamic community. Accumulating evidence suggests significant role for microorganisms cancer, therapies that incorporate bacteria have been tried various types of cancer. We previously demonstrated cupredoxin azurin secreted by opportunistic pathogen Pseudomonas aeruginosa , enters cancer cells induces apoptotic death 1–4 . However, physiological interactions between P. humans their tumor homeostasis are...
The use of live bacteria in the treatment cancer has a long and interesting history. We report purified bacterial redox protein, azurin, that enters human (melanoma UISO-Mel-2) cells induces apoptosis. induction apoptosis occurs readily melanoma harboring functional tumor suppressor protein p53, but much less efficiently p53-null mutant (UISO-Mel-6) cells. A redox-negative form azurin (M44K/M64E) demonstrates cytotoxicity to UISO-Mel-2 than wild-type protein. Azurin been shown be...
Digital superconductor electronics has been experiencing rapid maturation with the emergence of smaller-scale, lower-cost communications applications which became major technology drivers. These are primarily in area wireless communications, radar, and surveillance as well imaging sensor systems. In these areas, fundamental advantages superconductivity translate into system benefits through novel Digital-RF architectures direct digitization wide band, high frequency radio (RF) signals. At...
Abstract We report that amino acids 50 to 77 of azurin (p28) preferentially enter the human breast cancer cell lines MCF-7, ZR-75-1, and T47D through a caveolin-mediated pathway. Although p28 enters p53 wild-type MCF-7 isogenic dominant-negative MDD2 lines, only induces G2-M-phase cycle arrest apoptosis in cells. exerts its antiproliferative activity by reducing proteasomal degradation formation p28:p53 complex within hydrophobic DNA-binding domain (amino 80-276), increasing levels activity....
p28 is an anionic cell-penetrating peptide of 28 amino acids that activates wild-type and mutated p53, leading subsequently to selective inhibition CDK2 cyclin A expression G2-M cell-cycle arrest. In this study, we investigated the cytotoxic effects treatment alone in combination with DNA-damaging antimitotic agents on human cancer cells. enhanced activity lower concentrations (IC20-50) drugs (doxorubicin, dacarbazine, temozolamide) or (paclitaxel docetaxel) a variety cells expressing p53....
Azurin is a member of group copper-containing redox proteins called cupredoxins. Different cupredoxins are produced by different aerobic bacteria as agents electron transfer. Recently, we demonstrated that azurin enters into J774 and several types cancer cells leading to the induction apoptosis. We now demonstrate internalized in or temperature-dependent manner. shows preferential entry compared with normal cells. An 28-amino-acid fragment fused glutathione S-transferase (GST) green...
The tumor suppressor protein p53 is known to induce either apoptosis or growth arrest depending on cellular background. We have previously reported that a bacterial redox azurin induces in J774 cell line-derived macrophages whereas site-directed mutant M44KM64E shows very little cytotoxicity and fails cells. now report purified can enter both cells as well the human breast cancer MCF-7 Entry of causes strong inhibition cell-cycle progression at G 1 S phase higher level transcription p21...