A5092029744- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Identification and Quantification in Food
- Genomics and Phylogenetic Studies
- Food composition and properties
- Microbial Metabolites in Food Biotechnology
- Pesticide Residue Analysis and Safety
- Genetics, Bioinformatics, and Biomedical Research
- Isotope Analysis in Ecology
Fachhochschule Wiener Neustadt
2024
BOKU University
2023
Abstract High-resolution mass spectrometers, particularly when paired with liquid chromatography, are the instrument of choice for untargeted metabolomics approaches. Instruments, such as Orbitrap, offer high sensitivity, selectivity, and exceptional accuracy, though they pose certain technical challenges, complicating absolute comparative quantification. Consequently, method validation is crucial to ensure reliable results, approaches require detection quantification a large number...
Lyophilization is a common method used for stabilizing biological samples prior to storage or concentrate extracts. However, it possible that this process may alter the metabolic composition lead loss of metabolites. In study, performance lyophilization investigated in example wheat roots. To end, native and 13C-labelled, fresh already lyophilized root samples, (diluted) extracts with dilution factors up 32 authentic reference standards were investigated. All analyzed using RP-LC-HRMS....
Depending on the respective research question, LC-MS/MS based bottom-up proteomics poses challenges from initial biological sample all way to data evaluation. The focus of this study was investigate influence preparation techniques and analysis parameters protein identification in Tribolium castaneum by applying free software platform Max Quant.Different buffer systems (pH 4.8/8/10.8; with without urea Triton X-100) were tested for their extraction capacity - regarding total yield...
Depending on the respective research question, LC-MS/MS based bottom-up proteomics poses challenges from initial biological sample all way to data evaluation. The focus of this study was investigate influence preparation techniques and analysis parameters protein identification in Tribolium castaneum by applying free software platform Max Quant. Multidimensional extraction strategies combination with electrophoretic or chromatographic off-line pre-fractionation were applied enhance spectrum...