A5068068565- Transgenic Plants and Applications
- HIV/AIDS drug development and treatment
- HIV Research and Treatment
- Herpesvirus Infections and Treatments
- HIV/AIDS Research and Interventions
- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Immune Cell Function and Interaction
- Reproductive tract infections research
St George's, University of London
2005-2021
London School of Hygiene & Tropical Medicine
2021
St George's Hospital
2009
University of London
2009
Heterosexual transmission of human immunodeficiency virus (HIV) remains the major route infection worldwide; thus, there is an urgent need for additional prevention strategies, particularly strategies that could be controlled by women, such as topical microbicides. Potential microbicide candidates must both safe and effective. Using cellular tissue explant models, we have evaluated activity nonnucleoside reverse transcriptase inhibitor (NNRTI) dapivirine a vaginal microbicide. In...
Cyanovirin-N (CV-N) is a microbicide candidate that inactivates wide range of HIV strains by binding to gp120. Production CV-N, or any protein microbicide, needs be at extremely high levels and low cost have an impact on global health. Thus, it unlikely fermentor-based systems will suitable, including recombinant E. coli, where CV-N aggregates dimers consistently been found. Transgenic plants may provide suitable expression system for microbicides, as production can easily economically...
Many promising microbicide candidates are proteins or peptides, including neutralizing monoclonal antibodies (mAbs). Here, the expression of HIV-neutralizing mAb b12 in transgenic plants is described. The plant-derived was shown to have gp120 binding activity and vitro. However, it likely that a protein-based will need comprise combination two more products, order provide long-lasting cross-clade protection. Building on address for combinational agent, fusion protein with cyanovirin-N,...
ABSTRACT Topical blockade of the gp41 fusogenic protein HIV-1 is one possible strategy by which microbicides could prevent HIV transmission, working early against infection, inhibiting viral entry into host cells. In this study, we examined potential fusion inhibitors (FIs) as candidate anti-HIV microbicides. Preclinical evaluation four FIs, C34, T20, T1249, and L'644, was performed using cellular ex vivo genital colorectal tissue explant models. Increased sustained activity detected for a...
Abstract Background The continued growth of the global HIV epidemic highlights urgent need to develop novel prevention strategies reduce transmission. development topical microbicides is likely take a number years before such product would be widely available. This has resulted in call for rapid introduction simpler vaginal intervention interim period. One suggested practice douching with natural products including lime or lemon juice. Here we present comprehensive preclinical evaluation...
Objective: Small molecule inhibitors able to bind gp120 and prevent CD4 + -induced HIV-1 envelope conformational change provide an important class of inhibitors. Currently, only Fostemsavir is approved for HAART, which makes this attractive candidates prevention. We assessed the activity DS003 (BMS-599793), analogue BMS-378806, in different mucosal tissues elucidated its mechanism action. Design: Preclinical analysis was performed with human tissue models as surrogates in-vivo activity....