A5006355922- Cancer-related gene regulation
- Kruppel-like factors research
- Epigenetics and DNA Methylation
- Mitochondrial Function and Pathology
- Pluripotent Stem Cells Research
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Photosynthetic Processes and Mechanisms
- Electrostatics and Colloid Interactions
- Pancreatic function and diabetes
- RNA Interference and Gene Delivery
- Liver physiology and pathology
- Metalloenzymes and iron-sulfur proteins
- Renal and related cancers
- Ionic liquids properties and applications
- Hemoglobinopathies and Related Disorders
- Electrochemical Analysis and Applications
- Lipid metabolism and biosynthesis
- Genetic Syndromes and Imprinting
- Cytokine Signaling Pathways and Interactions
- Zebrafish Biomedical Research Applications
- Neuroendocrine Tumor Research Advances
- RNA Research and Splicing
- Various Chemistry Research Topics
- Cancer Genomics and Diagnostics
Novartis (Switzerland)
1971-2024
Icahn School of Medicine at Mount Sinai
2006-2023
Novartis Institutes for BioMedical Research
2019-2021
Institut de Génétique Moléculaire de Montpellier
2013
Pathways Behavioral Services
2009
Stockholm University
1998-1999
Freie Universität Berlin
1999
Symmetrical dimethylation on arginine-3 of histone H4 (H4R3me2s) has been reported to occur at several repressed genes, but its specific regulation and genomic distribution remained unclear. Here, we show that the type-II protein arginine methyltransferase PRMT5 controls H4R3me2s in mouse embryonic fibroblasts (MEFs). In these differentiated cells, find genome-wide pattern is highly similar stem cells. both cell types, peaks are detected predominantly G + C-rich regions. Promoters...
Abstract Transcription factor networks shape the gene expression programs responsible for normal cell identity and pathogenic state. Using Core Regulatory Circuitry analysis (CRC), we identify PAX8 as a candidate oncogene in Renal Cell Carcinoma (RCC) cells. Validation of large-scale functional genomic screens confirms that silencing leads to decreased proliferation RCC lines. Epigenomic analyses PAX8-dependent cistrome demonstrate largely occupies active enhancer elements controlling genes...
Abstract Dynamic regulation of histone methylation by methyltransferases and demethylases plays a central role in regulating the fate embryonic stem (ES) cells. The H3K9 methyltransferase KMT1E, formerly known as ESET or Setdb1, is essential to development ablation Setdb1 gene results peri-implantation lethality prevents propagation ES However, Setdb1-null blastocysts do not display global changes DNA methylation, arguing against genome-wide defect. Here we show that conditional deletion...
Even though light is the driving force in photosynthesis, it also can be harmful to plants. The water-splitting photosystem II main target for this stress, leading inactivation of photosynthetic electron transport and photooxidative damage its reaction center. plant survives through an intricate repair mechanism involving proteolytic degradation replacement photodamaged center D1 protein. Based on experiments with isolated chloroplast thylakoid membranes core complexes, we report several...
The hierarchical progression of stem and progenitor cells to their more-committed progeny is mediated through cell-to-cell signaling pathways intracellular transcription factor activity. However, the mechanisms that govern genetic networks underlying lineage fate decisions differentiation programs remain poorly understood. Here we show how integration Bmp4 Gata activity controls hematopoiesis, as exemplified by regulation Eklf during establishment erythroid lineage. Utilizing transgenic...
The switch from proliferation to differentiation during the terminal stages of erythropoiesis is a tightly controlled process that relies in part on transcription factor-mediated activation cell cycle components. EKLF key factor necessary for initial establishment red phenotype. Here, we find also plays role subsequent process, as it induces p21(WAF1/CIP1) expression independent p53 regulate changes underlying erythroid maturation. activates p21 not only by directly binding an site within...
Abstract Resident adult epithelial stem cells maintain tissue homeostasis by balancing self-renewal and differentiation. The cell potential of human epidermal keratinocytes is retained in vitro but lost over time suggesting extrinsic intrinsic regulation. Transcription factor-controlled regulatory circuitries govern identity, are sufficient to induce pluripotency transdifferentiate cells. We investigate whether transcriptional circuitry also governs phenotypic changes within a given type...
Understanding how transcriptional regulators are themselves controlled is important in attaining a complete picture of the intracellular effects that follow signaling cascades during early development and cell-restricted differentiation. We have addressed this issue by focusing on regulation EKLF/KLF1, zinc finger transcription factor plays necessary role global erythroid gene expression. Using biochemical affinity purification, we identified DEK oncoprotein as critical interacts with an...
EKLF/KLF1 is an essential transcription factor that plays a global role in erythroid transcriptional activation. Regulation of KLF1 interest, as it displays highly restricted expression pattern, limited to cells and its progenitors. Here we use biochemical affinity purification identify the DDX5/p68 protein activator by virtue interaction with erythroid-specific DNAse hypersensitive site upstream enhancer element (EHS1). We further show this associates DEK CTCF. postulate range interactions...
Abstract EKLF/KLF1 is an essential transcription factor that plays a global role in erythroid transcriptional activation. It’s own regulation of interest, as it displays highly restricted expression pattern, limited to cells and its progenitors. Here we use biochemical affinity purification identify the Ddx5/p68 protein activator KLF1 by virtue interaction with erythroid-specific DNAse hypersensitive site upstream enhancer element (EHS1). We postulate range interactions other proteins known...